Innate Immune Response of Blood Cells in Patients With Pneumonia

NCT ID: NCT03231670

Last Updated: 2024-03-06

Basic Information

• Recruitment Status: COMPLETED
• Total Enrollment: 37 participants
• Study Classification: OBSERVATIONAL
• Study Start Date: 2017-10-20
• Study Completion Date: 2020-06-24

Brief Summary

Pulmonary bacterial infections such as exacerbations of chronic bronchitis, nosocomial and community-acquired pneumonia represent a major public health issue. Antibiotics have shown their efficacy by direct antimicrobial activity and their limit particularly in case of multidrug-resistant microorganisms or in treating patients with aggravating pathologies. Innate immunity could be an alternative or complementary therapeutic pathway. Innate immunity receptors bind universal and invariant microbial molecular patterns present in bacteria, virus, fungus or parasite. Toll-like Receptors (TLR) activation by microbial agonist stimulates the innate immunity response which results in the production of chemokines, cytokines, antimicrobial molecules and the recruitment of innate cells.

The " Pulmonary Infection and Innate Immunity " team of the Immunity and Infection Center in Lille (Group of Dr. Sirard and Carnoy) has a long expertise in the study of TLR5 and its agonist, the flagellin, a structural protein of bacterial flagella. TLR5 is expressed on the cell surface of macrophages, monocytes, dendritic and epithelial cells. Several studies in mice have shown the flagellin prophylactic potential during bacterial infections through a TLR5 dependent stimulation of innate immunity. Recently, the group of Dr. Sirard and Carnoy has shown that flagellin can be used in association with antibiotics to treat Streptococcus pneumoniae respiratory infections in mice. The results demonstrate that an agonist of TLR can increase the therapeutic index of an antibiotic and improve the pulmonary anti-infectious reaction. This innovative approach allows us to consider new antibacterial strategies where antibiotics have reached their limit (nosocomial infection, multidrug-resistant bacteria…). TLR agonists can activate multiple human cell type. Indeed, blood cells activation by TLR agonists have been recently characterized in healthy volunteers.

However, there is no available data on the ability of TLR agonists to activate cells from patients with infectious pneumopathies. A study in these patients is inevitable if one is to consider the therapeutic use of agonists in respiratory pathologies.

Conditions

Lobar Pneumonia

Study Design

• Observational Model Type: CASE_ONLY
• Study Time Perspective: PROSPECTIVE

Study Groups

lobar pneumonia

Inpatient with lobar pneumonia will undergo a blood sampling during their hospitalization and after resolution of the infection (2 Months)

Blood sampling

Intervention Type: PROCEDURE

5ml blood will be taken in addition to standard sampling

Interventions

Blood sampling

5ml blood will be taken in addition to standard sampling

Intervention Type: PROCEDURE

Eligibility Criteria

Inclusion Criteria

• Adult patient hospitalized in the department of pneumology for whom clinical, radiological and biological criteria confirm the diagnosis of lobar pneumonia
• Beneficiary of the French National Health Insurance Fund
• Signed informed consent form

Exclusion Criteria

• Patient under guardianship
• Patient with acute respiratory distress syndrome or septic shock
• Pregnant women
• Patient with HIV, HCV or Mycobacterium tuberculosis
• Transplanted patient receiving immunosuppressive therapy

• Minimum Eligible Age: 18 Years
• Eligible Sex: ALL
• Accepts Healthy Volunteers: No

Sponsors

Institut Pasteur de Lille

OTHER

Sponsor Role: collaborator

Lille Catholic University

OTHER

Sponsor Role: lead

Responsible Party

Responsibility Role: SPONSOR

Principal Investigators

Christophe Carnoy, PhD

Role: STUDY_DIRECTOR

Institut Pasteur de Lille

Jean-Claude Sirard, PhD

Role: STUDY_DIRECTOR

Institut Pasteur de Lille

Locations

Hôpital Saint-Philibert

Lomme, Hauts-de-France, France

Countries

France

Other Identifiers

RT-12

Identifier Type: -

Identifier Source: org_study_id