Zinc and/or Probiotic Supplementation of Rotavirus and Oral Polio Virus Vaccines

NCT ID: NCT01616693

Last Updated: 2018-12-26

Study Results

Results available

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Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

PHASE4

Total Enrollment

620 participants

Study Classification

INTERVENTIONAL

Study Start Date

2012-07-31

Study Completion Date

2013-07-31

Brief Summary

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Background: Strategies are needed to improve oral rotavirus vaccine (RV), which provides suboptimal protection in developing countries. Probiotics and zinc supplementation could improve RV immunogenicity by altering the intestinal microbiota and immune function.

This study enrolled infants 5 weeks old living in urban Vellore, India to assess the effects of daily zinc (5 mg), probiotic (1010 Lactobacillus rhamnosus GG) or placebo on the immunogenicity of two doses of RV (Rotarix,GlaxoSmithKline Biologicals) given at 6 and 10 weeks of age. Probiotics and zinc (or placebo) were provided for six weeks. A single dose of test product was administered daily one week prior to first study dose of rotavirus and polio vaccines through 1 week following second study dose of rotavirus and polio vaccines.

Detailed Description

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Co- Primary objectives:

1. To evaluate the serologic immune response to rotavirus vaccine (sero-conversion or four-fold rise in rotavirus immunoglobulin A (IgA) antibodies) among Indian infants receiving zinc supplementation given daily for a week prior to the administration of the first dose through a week following the second dose of oral rotavirus vaccine compared to those receiving a zinc placebo.
2. To evaluate the serologic immune response to rotavirus vaccine (sero-conversion or four-fold rise in rotavirus IgA antibodies) among Indian infants receiving probiotic supplementation given daily for a week prior to the administration of the first dose through a week following the second dose of oral rotavirus vaccine compared to those receiving a probiotic placebo.

Conditions

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Immunity to Oral Rotavirus Vaccine Immunity to Oral Polio Vaccine Shedding of Oral Rotavirus Vaccine

Keywords

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rotavirus polio zinc supplement probiotic supplement rotavirus vaccine shedding

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

PARALLEL

Primary Study Purpose

BASIC_SCIENCE

Blinding Strategy

QUADRUPLE

Participants Caregivers Investigators Outcome Assessors

Study Groups

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Zinc and probiotic

Received daily zinc and probiotic supplements, in addition to rotavirus vaccine and trivalent oral polio vaccines.

Group Type EXPERIMENTAL

Probiotic

Intervention Type DIETARY_SUPPLEMENT

A capsule that contains at least 1 x 10\^9 Lactobacillus rhamnosus GG organisms per capsule. The contents of this capsule were given once daily orally.

Zinc

Intervention Type DIETARY_SUPPLEMENT

Zinc sulphate syrup is zinc sulphate heptahydrate (concentration 1mg/ml). 5 ml of this suspension was given once daily orally.

Rotavirus vaccine

Intervention Type BIOLOGICAL

1 ml Rotarix®, a lyophilized human rotavirus vaccine reconstituted with calcium carbonate buffer provided orally at 6 and 10 weeks.

Oral polio vaccine

Intervention Type BIOLOGICAL

A liquid trivalent polio vaccine provided orally at 6 and 10 weeks.

Zinc alone

Received daily zinc and probiotic placebo supplement, in addition to rotavirus vaccine and trivalent oral polio vaccine.

Group Type ACTIVE_COMPARATOR

Zinc

Intervention Type DIETARY_SUPPLEMENT

Zinc sulphate syrup is zinc sulphate heptahydrate (concentration 1mg/ml). 5 ml of this suspension was given once daily orally.

Probiotic placebo

Intervention Type DIETARY_SUPPLEMENT

The probiotic placebo was manufactured by the same company that manufactured the probiotic and contained inulin powder but no Lactobacillus rhamnosus GG. The contents of the capsule were given once daily orally.

Rotavirus vaccine

Intervention Type BIOLOGICAL

1 ml Rotarix®, a lyophilized human rotavirus vaccine reconstituted with calcium carbonate buffer provided orally at 6 and 10 weeks.

Oral polio vaccine

Intervention Type BIOLOGICAL

A liquid trivalent polio vaccine provided orally at 6 and 10 weeks.

Probiotic alone

Received daily zinc placebo and probiotic supplement, in addition to rotavirus vaccine and trivalent oral polio vaccine.

Group Type ACTIVE_COMPARATOR

Probiotic

Intervention Type DIETARY_SUPPLEMENT

A capsule that contains at least 1 x 10\^9 Lactobacillus rhamnosus GG organisms per capsule. The contents of this capsule were given once daily orally.

Zinc placebo

Intervention Type DIETARY_SUPPLEMENT

The zinc placebo excluded the zinc sulphate but contained lactose and was diluted to match the taste. 5 ml of this suspension was given orally once daily.

Rotavirus vaccine

Intervention Type BIOLOGICAL

1 ml Rotarix®, a lyophilized human rotavirus vaccine reconstituted with calcium carbonate buffer provided orally at 6 and 10 weeks.

Oral polio vaccine

Intervention Type BIOLOGICAL

A liquid trivalent polio vaccine provided orally at 6 and 10 weeks.

Placebo

Received daily zinc placebo and probiotic placebo, in addition to rotavirus vaccine and trivalent oral polio vaccine.

Group Type PLACEBO_COMPARATOR

Probiotic placebo

Intervention Type DIETARY_SUPPLEMENT

The probiotic placebo was manufactured by the same company that manufactured the probiotic and contained inulin powder but no Lactobacillus rhamnosus GG. The contents of the capsule were given once daily orally.

Zinc placebo

Intervention Type DIETARY_SUPPLEMENT

The zinc placebo excluded the zinc sulphate but contained lactose and was diluted to match the taste. 5 ml of this suspension was given orally once daily.

Rotavirus vaccine

Intervention Type BIOLOGICAL

1 ml Rotarix®, a lyophilized human rotavirus vaccine reconstituted with calcium carbonate buffer provided orally at 6 and 10 weeks.

Oral polio vaccine

Intervention Type BIOLOGICAL

A liquid trivalent polio vaccine provided orally at 6 and 10 weeks.

Interventions

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Probiotic

A capsule that contains at least 1 x 10\^9 Lactobacillus rhamnosus GG organisms per capsule. The contents of this capsule were given once daily orally.

Intervention Type DIETARY_SUPPLEMENT

Zinc

Zinc sulphate syrup is zinc sulphate heptahydrate (concentration 1mg/ml). 5 ml of this suspension was given once daily orally.

Intervention Type DIETARY_SUPPLEMENT

Probiotic placebo

The probiotic placebo was manufactured by the same company that manufactured the probiotic and contained inulin powder but no Lactobacillus rhamnosus GG. The contents of the capsule were given once daily orally.

Intervention Type DIETARY_SUPPLEMENT

Zinc placebo

The zinc placebo excluded the zinc sulphate but contained lactose and was diluted to match the taste. 5 ml of this suspension was given orally once daily.

Intervention Type DIETARY_SUPPLEMENT

Rotavirus vaccine

1 ml Rotarix®, a lyophilized human rotavirus vaccine reconstituted with calcium carbonate buffer provided orally at 6 and 10 weeks.

Intervention Type BIOLOGICAL

Oral polio vaccine

A liquid trivalent polio vaccine provided orally at 6 and 10 weeks.

Intervention Type BIOLOGICAL

Other Intervention Names

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Culturelle Rotarix® Biopolio®

Eligibility Criteria

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Inclusion Criteria

* Infants 35-41 days old
* Live in area under surveillance
* Current weight ≥3.2 kg
* No syndromic evidence of immunocompromise as determined by medical doctor
* No prior illness requiring hospitalization
* No current medical condition as determined by medical doctor which precludes study involvement
* Available for follow up for duration of study (through approximately 14 weeks of age)
* Parents/guardians of infant are able to understand and follow study procedures and agree to participate in the study by providing signed informed consent

Exclusion Criteria

* Child has history of atopic symptoms
* Child has a known digestive system defect
* Child has history of chronic diarrhea
* Child has major congenital anomalies
* Child has received a prior dose of rotavirus vaccine
* Child has received a prior dose of polio vaccine (beyond the birth dose)
Minimum Eligible Age

5 Weeks

Maximum Eligible Age

16 Weeks

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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Christian Medical College, Vellore, India

OTHER

Sponsor Role collaborator

Ministry of Science and Technology, India

OTHER_GOV

Sponsor Role collaborator

PATH

OTHER

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Gagandeep Kang, MD, PhD

Role: PRINCIPAL_INVESTIGATOR

Christian Medical Center, Vellore, India

Locations

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Christian Medical Center, Vellore

Vellore, Tamil Nadu, India

Site Status

Countries

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India

Other Identifiers

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CMC ZP 2012; PATH HS-658

Identifier Type: -

Identifier Source: org_study_id