Antiviral Activity of Oral Probiotics

NCT ID: NCT05374070

Last Updated: 2022-05-13

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

UNKNOWN

Clinical Phase

NA

Total Enrollment

20 participants

Study Classification

INTERVENTIONAL

Study Start Date

2022-05-23

Study Completion Date

2022-08-15

Brief Summary

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The aim of this study is to test saliva samples obtained from healthy human participants for anti-viral activity after they have consumed S. salivarius probiotic in a powder format.

Detailed Description

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This is a randomized controlled open pilot study to evaluate saliva samples collected from participants following their consumption of powders containing the commercially marketed probiotic bacterium S. salivarius K12 or S. salivarius M18.

Participants will be randomly assigned to one of the 4 groups consuming probiotic powder containing Streptococcus salivarius K12 or S. salivarius M18 with or without prebiotics (e.g. sugars that may promote probiotic activity). Saliva samples will be collected at predetermined time points pre and post intervention. Antibacterial and Antiviral activity will be determined following established assays. Colonisation efficacy will be determined by enumerating the probiotic in the saliva samples using standard microbiological techniques.

Conditions

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Lower Respiratory Tract Infection Upper Respiratory Tract Infection

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

PARALLEL

Participants will be randomly assigned to one of the 4 groups consuming powder containing Streptococcus salivarius K12 or S. salivarius M18 with or without prebiotic:

1. Probiotic Streptococcus salivarius K12 Powder (Dose: 1 Billion cfu/g)
2. Probiotic Streptococcus salivarius K12 Powder with prebiotic (Dose: 1 Billion cfu/g)
3. Probiotic Streptococcus salivarius M18 Powder (Dose: 1 Billion cfu/g)
4. Probiotic Streptococcus salivarius M18 Powder with prebiotic (Dose: 1 Billion cfu/g)
Primary Study Purpose

BASIC_SCIENCE

Blinding Strategy

NONE

Study Groups

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Streptococcus salivarius K12

Streptococcus salivarius K12 (Dose 1: 1 Billion colony forming units /g)

Group Type ACTIVE_COMPARATOR

Streptococcus salivarius K12 Powder 1 Billion colony forming units /g)

Intervention Type DIETARY_SUPPLEMENT

Probiotic Streptococcus salivarius K12 products are commercially available in traditional formats such as lozenges for local delivery in the oral cavity to provide oral health benefits. In this study, a powder formulation will be evaluated for its potential of delivering probiotic Streptococcus salivarius K12 to the oral cavity.

Streptococcus salivarius K12 with a prebiotic included

Streptococcus salivarius K12 (Dose 1: 1 Billion colony forming units /g)

Group Type ACTIVE_COMPARATOR

Streptococcus salivarius K12 Powder (1 Billion colony forming units /g) with a prebiotic included

Intervention Type DIETARY_SUPPLEMENT

In this study, a powder formulation containing Streptococcus salivarius K12 and a prebiotic will be evaluated for its potential of delivering probiotic Streptococcus salivarius K12 to the oral cavity.

Streptococcus salivarius M18

Streptococcus salivarius M18 (Dose 1: 1 Billion colony forming units /g)

Group Type ACTIVE_COMPARATOR

Streptococcus salivarius M18 Powder (1 Billion colony forming units /g)

Intervention Type DIETARY_SUPPLEMENT

In this study, a powder formulation will be evaluated for its potential of delivering probiotic Streptococcus salivarius M18 to the oral cavity.

Streptococcus salivarius M18 with a prebiotic included

Streptococcus salivarius M18 (Dose 1: 1 Billion colony forming units /g)

Group Type ACTIVE_COMPARATOR

Streptococcus salivarius M18 Powder (1 Billion colony forming units /g) with a prebiotic included

Intervention Type DIETARY_SUPPLEMENT

In this study, a powder formulation containing S. salivarius M18 and a prebiotic will be evaluated for its potential of delivering probiotic Streptococcus salivarius M18 to the oral cavity.

Interventions

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Streptococcus salivarius K12 Powder 1 Billion colony forming units /g)

Probiotic Streptococcus salivarius K12 products are commercially available in traditional formats such as lozenges for local delivery in the oral cavity to provide oral health benefits. In this study, a powder formulation will be evaluated for its potential of delivering probiotic Streptococcus salivarius K12 to the oral cavity.

Intervention Type DIETARY_SUPPLEMENT

Streptococcus salivarius K12 Powder (1 Billion colony forming units /g) with a prebiotic included

In this study, a powder formulation containing Streptococcus salivarius K12 and a prebiotic will be evaluated for its potential of delivering probiotic Streptococcus salivarius K12 to the oral cavity.

Intervention Type DIETARY_SUPPLEMENT

Streptococcus salivarius M18 Powder (1 Billion colony forming units /g)

In this study, a powder formulation will be evaluated for its potential of delivering probiotic Streptococcus salivarius M18 to the oral cavity.

Intervention Type DIETARY_SUPPLEMENT

Streptococcus salivarius M18 Powder (1 Billion colony forming units /g) with a prebiotic included

In this study, a powder formulation containing S. salivarius M18 and a prebiotic will be evaluated for its potential of delivering probiotic Streptococcus salivarius M18 to the oral cavity.

Intervention Type DIETARY_SUPPLEMENT

Eligibility Criteria

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Inclusion Criteria

1. In general good health 18 - 80 years of age.
2. Practice good oral hygiene.

Exclusion Criteria

1. Have a history of autoimmune disease or are immunocompromised.
2. Are on concurrent antibiotic therapy or regular antibiotic use within last 1 week
3. History of allergy (e.g. dairy).
Minimum Eligible Age

18 Years

Maximum Eligible Age

80 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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John Hale

INDUSTRY

Sponsor Role lead

Responsible Party

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John Hale

Chief Technology Officer

Responsibility Role SPONSOR_INVESTIGATOR

Principal Investigators

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John D Hale, PhD

Role: STUDY_DIRECTOR

Blis Technologies Ltd, Dunedin, New Zealand

John R Tagg, PhD

Role: PRINCIPAL_INVESTIGATOR

Blis Technologies Ltd, Dunedin, New Zealand

Liam Harold, PhD

Role: PRINCIPAL_INVESTIGATOR

Blis Technologies Ltd, Dunedin, New Zealand

Locations

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Blis Technologies Ltd

Dunedin, Otago, New Zealand

Site Status RECRUITING

Countries

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New Zealand

Central Contacts

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John D Hale, PhD

Role: CONTACT

6434740988

John R Tagg, PhD

Role: CONTACT

6434740988

Facility Contacts

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John D Hale, PhD

Role: primary

+6434740988

Liam Harold, PhD

Role: backup

+6434740988

References

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Hyink O, Wescombe PA, Upton M, Ragland N, Burton JP, Tagg JR. Salivaricin A2 and the novel lantibiotic salivaricin B are encoded at adjacent loci on a 190-kilobase transmissible megaplasmid in the oral probiotic strain Streptococcus salivarius K12. Appl Environ Microbiol. 2007 Feb;73(4):1107-13. doi: 10.1128/AEM.02265-06. Epub 2006 Dec 28.

Reference Type BACKGROUND
PMID: 17194838 (View on PubMed)

Burton JP, Chilcott CN, Wescombe PA, Tagg JR. Extended Safety Data for the Oral Cavity Probiotic Streptococcus salivarius K12. Probiotics Antimicrob Proteins. 2010 Oct;2(3):135-44. doi: 10.1007/s12602-010-9045-4.

Reference Type BACKGROUND
PMID: 26781236 (View on PubMed)

Other Identifiers

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BLTCT2022/2

Identifier Type: -

Identifier Source: org_study_id

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