Probiotic, Prebiotic and Synbiotic Effect on Immunity

NCT ID: NCT01084434

Last Updated: 2010-03-10

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

UNKNOWN

Clinical Phase

NA

Total Enrollment

100 participants

Study Classification

INTERVENTIONAL

Study Start Date

2010-05-31

Study Completion Date

2011-06-30

Brief Summary

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The aim of the study is to investigate the effect of dietary supplements such as probiotic, prebiotic and synbiotic on the immune response to influenza vaccination and faecal microbiota in adult healthy volunteers.

Detailed Description

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Within recent times, influenza has become a major respiratory infection worldwide. The preventative vaccination reduces the severity of infection but ageing reduced its efficacy and it is only effective in 17-53% in elderly individuals. Thus, nutritionists and medical researchers are looking for opportunities to improve the immune response to influenza vaccine. Functional foods, such as probiotics, prebiotics and synbiotics, apart from other health benefits, may contribute towards immune protection.

Probiotics are beneficial bacteria, which are regarded as safe and serve health benefits to the host, while prebiotics are carbohydrates which by escaping digestion in the upper gastrointestinal tract may reach the colon and there serve as food for beneficial bacteria in the colon. When prebiotic used in combination with probiotic bacteria, it is called synbiotic, and it may improve survival and implantation of probiotic in the gut.

The human study will examine the immune response to vaccination and changes in faecal microbiota during administration of probiotic, prebiotic and synbiotic supplements. The study will be conducted between vaccinations seasons in 2010-2011 and recruit 100 healthy subjects aged 40-65years old. Participant will be given one of four different treatments over a 7 week period following 3 weeks of wash-out period. Treatments given include: a probiotic, a prebiotic, a synbiotic and a placebo. In the 3rd week of product consumption the participant will be given a flu jab. Specific antibody titre against the 3 viral strains composing the vaccine and total immunoglobulin concentration in the serum will be monitored during 2 and 4 weeks after vaccination. Faecal samples and saliva will be collected and analysed for changes in faecal microbial populations.

Conditions

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Dietary Intervention

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

PARALLEL

Primary Study Purpose

TREATMENT

Blinding Strategy

DOUBLE

Participants Investigators

Study Groups

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Probiotic group

Group of 25 volunteers consuming probiotic product once a day for 7 weeks

Group Type EXPERIMENTAL

probiotic

Intervention Type DIETARY_SUPPLEMENT

Bifidobacterium lactis HN019 10\^9 CFU/day 1 sachet once a day for 7 weeks

Prebiotic group

Group of 25 volunteers consuming prebiotic product once a day for 7 weeks

Group Type EXPERIMENTAL

prebiotic

Intervention Type DIETARY_SUPPLEMENT

Galactooligosaccharide 5.5 g/day 1 sachet once a day for 7 weeks

Synbiotic group

Group of 25 volunteers consuming synbiotic product once a day for 7 weeks.

Group Type EXPERIMENTAL

Synbiotic

Intervention Type DIETARY_SUPPLEMENT

(Bifidobacterium lactis 10\^9 CFU + Galactooligosaccharide 5.5g) / day - 1 sachet once a day for 7 weeks

Placebo group

Group of 25 volunteers consuming placebo product once a day for 7 weeks

Group Type PLACEBO_COMPARATOR

Placebo

Intervention Type DIETARY_SUPPLEMENT

Maltodextrin 5.5g/day - 1 sachet once a day for 7 weeks

Interventions

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probiotic

Bifidobacterium lactis HN019 10\^9 CFU/day 1 sachet once a day for 7 weeks

Intervention Type DIETARY_SUPPLEMENT

prebiotic

Galactooligosaccharide 5.5 g/day 1 sachet once a day for 7 weeks

Intervention Type DIETARY_SUPPLEMENT

Synbiotic

(Bifidobacterium lactis 10\^9 CFU + Galactooligosaccharide 5.5g) / day - 1 sachet once a day for 7 weeks

Intervention Type DIETARY_SUPPLEMENT

Placebo

Maltodextrin 5.5g/day - 1 sachet once a day for 7 weeks

Intervention Type DIETARY_SUPPLEMENT

Other Intervention Names

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HOWARU Bifidobacterium lactis Bimuno

Eligibility Criteria

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Inclusion Criteria

* Signed consent form
* Age 40-65 years
* Body mass index 18.5-30 inclusive
* Good general health as determined by medical questionnaires
* Not vaccinated with the current seasonal influenza (2009) or swine flu vaccine

Exclusion Criteria

* Evidence of physical or mental disease or planned major surgery, which might limit participation in or completion of the study
* History of drug misuse, including alcohol
* Allergy to the vaccine
* Asplenia and other acquired or congenital immunodeficiency
* Severe allergy such as asthma, hay-fever, dermatitis or being treated on these
* History of severe abnormal drug reaction
* Any autoimmune disease
* Diabetic (type 1 or type2)
* Food allergy manifested by gastrointestinal, skin, respiratory , neurological, anaphylaxis symptoms
* Lactose intolerance showed by clinical symptoms such as nausea, cramping, bloating, diarrhea and flatulence after consuming lactose containing dairy products (milk, yoghurt, butter, cheese, ice-cream, sour cream) or lactose non-dairy products ( whey, milk solids, modified milk ingredients)
* Participation in experimental drug trial within four weeks prior to study
* Participation in prebiotics or laxative trial within the previous three months
* Use of antibiotics within the previous six months
* Chronic constipation, diarrhoea or other chronic gastro-intestinal complaint
* Intake of other prebiotics or probiotics, drugs active on gastrointestinal motility, or a laxative of any class for four weeks prior to study
* Use of prescribed medication
* Regular use of aspirin or other anti-inflammatory drugs
Minimum Eligible Age

45 Years

Maximum Eligible Age

65 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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University of Reading

OTHER

Sponsor Role lead

Responsible Party

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University of Reading

Principal Investigators

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Maria Magdalena Baran, MSc

Role: PRINCIPAL_INVESTIGATOR

Sofia Kolida, PhD

Role: PRINCIPAL_INVESTIGATOR

Locations

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University of Reading, Food and Nutritional Sciences Department

Reading, Berkshire, United Kingdom

Site Status

Countries

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United Kingdom

Central Contacts

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Maria Magdalena Baran, MSc

Role: CONTACT

0044118 378 6217

Other Identifiers

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F3168407

Identifier Type: -

Identifier Source: org_study_id

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