Assessment of Oxidative Stress Contents in Jordanian Pregnant Women With Preeclampsia

NCT ID: NCT07119034

Last Updated: 2025-08-12

Basic Information

• Recruitment Status: COMPLETED
• Total Enrollment: 90 participants
• Study Classification: OBSERVATIONAL
• Study Start Date: 2022-11-01
• Study Completion Date: 2023-05-01

Brief Summary

Preeclampsia, a widespread complication affecting pregnant women globally, necessitates a thorough investigation into the role of oxidative stress in its manifestation. This study aimed to assess the oxidative stress markers-malondialdehyde (MDA), 4-hydroxynonenal (4-HNE), and thioredoxin (Trx) in preeclamptic compared to healthy pregnant women

Detailed Description

Preeclampsia, a widespread complication affecting pregnant women globally, necessitates a thorough investigation into the role of oxidative stress in its manifestation. This study aimed to assess the oxidative stress markers-malondialdehyde (MDA), 4-hydroxynonenal (4-HNE), and thioredoxin (Trx) in preeclamptic compared to healthy pregnant women.

Methods: Ethical approval was obtained from Al-Ahliyya Amman University prior to starting the study. A total of 90 participants (45 preeclamptic pregnant women and 45 healthy pregnant women) were included in this study. Serum levels of total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), and high-density lipoprotein cholesterol (HDL-C) were measured by HUMAN200 auto analyzer (Germany) using a homogeneous enzymatic colorimetric assay. A kit from GenoChem world was used to measure the MDA levels via a colorimetric analysis technique Sandwich enzyme-linked immunosorbent assay (ELISA) was used to determine the serum levels of Trx and 4-HNE.

Results: The results revealed significantly elevated levels of MDA and 4-HNE in preeclamptic women compared to their healthy counterparts (P<0.001). Although Trx levels did not differ significantly (P=0.29), marked distinctions were observed in LDL-C, TC, HDL-C, systolic and diastolic blood pressure (P<0.001). Preeclamptic women exhibited substantially higher LDL, TC, and blood pressure, and lower HDL.

Conclusion: This study highlights the correlation between elevated oxidative stress markers and an elevated risk of preeclampsia in pregnant women, which may suggest the impact of these markers in the pathogenesis of preeclampsia. These findings underscore the need for a nuanced understanding of these markers to enhance prenatal care and facilitate targeted interventions

Conditions

Preeclampsia (PE); Oxidative Stress

Study Design

• Observational Model Type: CASE_CONTROL
• Study Time Perspective: PROSPECTIVE

Study Groups

Control group

control group comprising 45 healthy pregnant women

Total cholesterol (TC), random blood glucose (RBG), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), and high-density lipoprotein cholesterol (HDL-C)

Intervention Type: DIAGNOSTIC_TEST

Blood samples were collected from the antecubital vein and processed at the TrueLab medical laboratory. The whole blood was initially collected in plain tubes, and subsequently centrifuged to obtain serum samples. Total cholesterol (TC), random blood glucose (RBG), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), and high-density lipoprotein cholesterol (HDL-C) Thioredoxin and 4-Hydroxynonenal quantification, malondialdehyde levels

Preeclampsia group

Preeclampsia group 45 women with preeclampsia

Total cholesterol (TC), random blood glucose (RBG), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), and high-density lipoprotein cholesterol (HDL-C)

Intervention Type: DIAGNOSTIC_TEST

Blood samples were collected from the antecubital vein and processed at the TrueLab medical laboratory. The whole blood was initially collected in plain tubes, and subsequently centrifuged to obtain serum samples. Total cholesterol (TC), random blood glucose (RBG), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), and high-density lipoprotein cholesterol (HDL-C) Thioredoxin and 4-Hydroxynonenal quantification, malondialdehyde levels

Interventions

Total cholesterol (TC), random blood glucose (RBG), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), and high-density lipoprotein cholesterol (HDL-C)

Blood samples were collected from the antecubital vein and processed at the TrueLab medical laboratory. The whole blood was initially collected in plain tubes, and subsequently centrifuged to obtain serum samples. Total cholesterol (TC), random blood glucose (RBG), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), and high-density lipoprotein cholesterol (HDL-C) Thioredoxin and 4-Hydroxynonenal quantification, malondialdehyde levels

Intervention Type: DIAGNOSTIC_TEST

Eligibility Criteria

Exclusion Criteria

• applied to those with diabetes, liver or kidney diseases, cardiovascular diseases, or using specific supplements like vitamins A, C, and E.

• Minimum Eligible Age: 20 Years
• Maximum Eligible Age: 40 Years
• Eligible Sex: FEMALE
• Accepts Healthy Volunteers: Yes

Sponsors

Al-Ahliyya Amman University

OTHER

Sponsor Role: collaborator

Future University in Egypt

OTHER

Sponsor Role: lead

Responsible Party

Doaa Mohamed Salah ElDin Diab ELBohy

Assistant professor of clinical Pharmacy

Responsibility Role: PRINCIPAL_INVESTIGATOR

Principal Investigators

Doaa El-Bohy, PhD

Role: PRINCIPAL_INVESTIGATOR

Al-Ahliyya Amman University

Locations

Al-Ahliyya Amman University

Amman, , Jordan

Countries

Jordan

Other Identifiers

Preeclsmpsia

Identifier Type: REGISTRY

Identifier Source: secondary_id

Preeclampsia

Identifier Type: OTHER

Identifier Source: secondary_id

Al-Ahliyya Amman University

Identifier Type: -

Identifier Source: org_study_id