53-Week Study Evaluating the Safety and Efficacy of Intra-articular Injections of TPX-100 With Mild to Severe Tibio-Femoral Osteoarthritis of the Knee

NCT ID: NCT06865079

Last Updated: 2025-10-08

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

RECRUITING

Clinical Phase

PHASE2

Total Enrollment

270 participants

Study Classification

INTERVENTIONAL

Study Start Date

2025-05-09

Study Completion Date

2027-06-15

Brief Summary

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53-Week Study Evaluating the Safety and Efficacy of Intra-articular (IA) Injections of TPX-100 vs Placebo in Subjects with Mild to Severe Tibio-Femoral Osteoarthritis of the Knee

Detailed Description

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This multicenter, double-blind, placebo controlled, randomized study is designed to investigate the safety and efficacy of TPX-100 administered in 4 weekly doses of 200 mg per dose in subjects with tibio-femoral osteoarthritis. The B-score as determined by MRI analysis is the final inclusion criteria, which will be determined by MRI-based assessment. A standardized screening MRI will be obtained on otherwise eligible candidates.

For enrolled subjects, the Screening MRI will serve as the Baseline MRI. Enrolled subjects will be assigned randomly to receive either TPX-100 or PBS placebo, 4 weekly IA injections administered into the target knee by fluoroscopy or ultrasound guidance.

Subjects will undergo follow-up knee MRIs at Week 27 and Week 53 after the first injection. All MRIs will be read by computer-based, standardized methods, blind to treatment assignment and temporal sequence, to provide the imaging outcome measures. Subjects will complete PROs at Week 1 (Baseline), Week 27 and Week 53. A clinical safety evaluation will be performed at Week 13, and a safety tele-visit or telephone call will be performed at Week 36. Adverse events, regardless of causation, and concomitant medications will be documented at all study visits and at the Week 37 tele-visit/telephone call. Clinical laboratory assessments will be obtained at Screening, Baseline (Week 1), and Weeks 2, 3, 4; and at the in-person follow up visits.

All enrolled subjects will be assigned randomly to receive either TPX-100 or PBS placebo, in 4 weekly IA injections administered into the target knee by fluoroscopy or ultrasound guidance.

Conditions

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Osteoarthritis (OA) of the Knee

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

PARALLEL

Randomized, Double-blind, Placebo-Controlled
Primary Study Purpose

TREATMENT

Blinding Strategy

QUADRUPLE

Participants Caregivers Investigators Outcome Assessors
Clinical Research Organizations (CROs) Sponsor

Study Groups

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TPX-100 (200 mg per dose)

TPX-100 (200 mg per dose) for intra-articular injection administered once-weekly (Week 1, 2, 3 and 4) to a single target knee of the subject for 4 injections.

Group Type EXPERIMENTAL

TPX-100 200mg, Once weekly for 4 weeks

Intervention Type DRUG

TPX-100 (200 mg per dose) will be administered once-weekly (Week 1, 2, 3 \& 4) to a single target knee of the subject for 4 injections.

TPX-100 can be administered by conventional methods such as subcutaneous or intra-articular injection.

Placebo / PBS

PBS placebo for intra-articular injection administered once-weekly (Week 1, 2, 3 and 4) to a single target knee of the subject for 4 injections.

Group Type PLACEBO_COMPARATOR

PBS

Intervention Type OTHER

PBS / placebo for intra-articular injection administered once-weekly (Week 1, 2, 3 and 4) to a single target knee of the subject for 4 injections

Interventions

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TPX-100 200mg, Once weekly for 4 weeks

TPX-100 (200 mg per dose) will be administered once-weekly (Week 1, 2, 3 \& 4) to a single target knee of the subject for 4 injections.

TPX-100 can be administered by conventional methods such as subcutaneous or intra-articular injection.

Intervention Type DRUG

PBS

PBS / placebo for intra-articular injection administered once-weekly (Week 1, 2, 3 and 4) to a single target knee of the subject for 4 injections

Intervention Type OTHER

Eligibility Criteria

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Inclusion Criteria

Volunteers will be included in the study only if they satisfy all the following criteria:

1. Able to read, understand, sign, and date the subject informed consent.
2. Have given written informed consent before any study-related activities are carried out and are able to understand the nature and purpose of the trial, including possible risks and adverse effects.
3. Adult males and females, 50 to 80 years of age (inclusive) at Screening.
4. Body weight less than 136 kgs (300 lbs) at Screening.
5. Except for OA, subject is medically healthy (in the opinion of the PI), as determined by prestudy medical history, and without clinically significant abnormalities including:

1. Clinically relevant findings on physical examination that would preclude trial compliance.
2. Heart rate \<40 BPM or \>100 BPM after 5 minutes rest in supine or semi-supine position.
3. Body temperature \<95.9°F or \>99.8°F. Note: The above assessments may be repeated, if abnormal values are recorded in the first instance, at the discretion of the Investigator (or delegate).
6. Available X-Ray of target knee obtained within 12 months prior to Screening with radiographic evidence of osteoarthritis in the medial and/or lateral tibio-femoral compartment; KL grading is not required.
7. Clinical diagnosis of knee OA with at least 2 of the 4 following signs/symptoms in the target knee, and in the absence of palpable warmth suggesting synovitis or infection:

* Crepitus on knee extension
* Bony tenderness
* Bony enlargement
* Morning stiffness in target knee \<30 minutes
8. Stability of Cruciate and collateral ligaments as defined by clinical examination (e.g. tests such as Lachman, pivot shift, posterior drawer, posterior sag, valgus and Varus stress).
9. Results of the KOOS Knee Survey Screening Questionnaire performed at Screening for the target knee that indicate either: moderate or greater difficulty in at least 5 of 17 questions (A1-17) in the "Function, daily living" subsection; or a total score of at least 20/68 in that subsection.
10. Results of the KOOS Knee Survey Screening Questionnaire performed at Screening that indicate moderate or greater pain on question P6 "pain going up or down stairs" in the target knee.
11. Willing to use only acetaminophen (Tylenol® or equivalent), hydrocodone, orhydrocodone/acetaminophen (e.g., Norco® or equivalent) for breakthrough pain during the4-week injection period. The maximum dose of acetaminophen from all sources must not exceed 4000 mg/day.
12. Willing NOT to use non-steroidal anti-inflammatory drugs such as aspirin, ibuprofen (Motrin® or equivalent), or naproxen (Aleve® or equivalent) during the 4-week injection period.
13. Willing NOT to use approved or investigational IA products for knee OA for the duration of participation in the study.
14. If using GLP-1 medications such as Wegovy® or similar, must be on a stable dose for at least 2 months prior to Screening.
15. Female subjects of childbearing potential who are sexually active (non-abstinent) must agree to and comply with using 2 highly effective methods of birth control (oral contraceptive implant, injectable or indwelling intrauterine device, condom with spermicide or sexual abstinence) while participating in the study. Male participants must agree to use a barrier contraception method to prevent pregnancy, and agree not to donate sperm from the time of the first injection through the end of the study.
16. Willing and able to comply with all study assessments and adhere to the protocol schedule and restrictions.
17. MRI Screening: Tibio-femoral osteoarthritis with MRI based femoral B-score at Screening of 0.5 or greater in at least one knee as determined by central analysis.

Exclusion Criteria

Subjects are excluded from the study if any of the following criteria are met:

1. Contraindication to MRI.
2. Demonstrated clinically significant (required intervention, e.g., emergency room visit, epinephrine administration) allergic reactions (e.g., food, drug, or atopic reactions, asthmatic episodes) which, in the opinion of the Investigator, would interfere with the volunteer's ability to participate in the trial.
3. Prior surgery in the target knee, excluding procedures for debridement only (no previous micro-fracture procedure).
4. Knee joint replacement or any other knee surgery planned in either knee while participating in the study.
5. Moderate or severe pain in the contralateral (non-target) knee.
6. History of fibromyalgia, rheumatoid arthritis (RA), psoriatic arthritis, or any other autoimmune or infectious cause for arthritis; or referred knee pain from hip or spinal disease.
7. Knee effusion \>2+ on the following clinical scale:

1. Zero = No wave produced on down stroke
2. Trace = Small wave on medial side with down stroke
3. 1+ = Larger bulge on medial side with down stroke
4. 2+ = Effusion spontaneously returned to medial side after upstroke (no down stroke necessary)
5. 3+ = So much fluid that it was not possible to move the effusion out of the medial aspect of the knee
8. Last viscosupplementation (e.g., Synvisc® or similar hyaluronic acid product) injected into either knee \< 3 months before Screening.
9. Last IA injection of corticosteroids \< 2 months before Screening.
10. Current (within previous month before Screening) of use of any systemic steroids (except inhaled corticosteroids for allergy or respiratory problems).
11. Known hypersensitivity to any of the study drug ingredients.
12. Known hypersensitivity to acetaminophen or hydrocodone.
13. History of arthroscopy in the target knee in the 3 months before Screening.
14. History of septic arthritis, gout, or pseudo-gout in either knee in the year before Screening.
15. Clinical signs in the opinion of the Investigator of acute meniscal tear (e.g. locking or acute mechanical signs or symptoms consistent with meniscal tear) in either knee.
16. Skin lesion, rash, infection, or hypersensitivity in the target knee at or near the injection site at Screening.
17. Bleeding problems, platelet or coagulation deficiency that, in the option of the Investigator, contraindicates IA injection.
18. Active systemic infection at Screening or immediately pre-dose on any IA injection day, including chronic viral infection.
19. Current treatment or treatment within 2 years prior to Screening for any malignancy except basal cell or squamous cell carcinoma of the skin, prostate or cervical cancer in situ, unless with specific written permission provided by the Sponsor's Medical Monitor.
20. Women of childbearing potential who are pregnant, nursing, or planning to become pregnant, and women or men who do not agree to remain on an acceptable method of birth control throughout the entire study period.
21. Participation in other clinical OA drug studies within 1 year prior to Screening except epidemiologic studies involving no drug treatment.
22. Any participation in other clinical OA stem cell or gene therapy studies.
23. Currently taking paclitaxel (mitotic inhibitor), natalizumab (anti-integrin monoclonal antibody), or any other anti-integrin treatment.
24. Routine consumption of more than 3 alcoholic drinks a day (definition of 1 alcoholic drink:

* 12-ounces of beer, 8-ounces of malt liquor, 5-ounces of wine, 1.5- ounces or a "shot" of 80-proof distilled spirits or liquor such as gin, rum, vodka, or whiskey).
25. History of substance abuse in the opinion of the Investigator within the two years prior to Screening.
Minimum Eligible Age

50 Years

Maximum Eligible Age

80 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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OrthoTrophix, Inc

INDUSTRY

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Dawn McGuire - Chief Medical Officer, M.D. FAAN

Role: STUDY_CHAIR

OrthoTrophix, Inc

Locations

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Elite Clinical Network

Scottsdale, Arizona, United States

Site Status RECRUITING

Noble Clinical Research

Tucson, Arizona, United States

Site Status RECRUITING

Legent Orthopedic Hospital

Carrolton, California, United States

Site Status RECRUITING

Medvin Clinical Research

Covina, California, United States

Site Status RECRUITING

CORE Orthopaedic Medical Center

Encinitas, California, United States

Site Status RECRUITING

Marvel Clinical Research (Elite Clinical Network)

Huntington Beach, California, United States

Site Status RECRUITING

Medvin Clinical Research Center

Riverside, California, United States

Site Status RECRUITING

Medvin Clinical Research Center

Tujunga, California, United States

Site Status RECRUITING

Medvin Clinical Research Center

Whittier, California, United States

Site Status RECRUITING

K2 Medical Research

Maitland, Florida, United States

Site Status RECRUITING

Wellness Research Center

Miami, Florida, United States

Site Status RECRUITING

Well Pharma Medical Research

Miami, Florida, United States

Site Status RECRUITING

Las Mercedes Medical Research

Miami, Florida, United States

Site Status RECRUITING

Drug Studies of America

Marietta, Georgia, United States

Site Status RECRUITING

AMR Kansas City

Kansas City, Kansas, United States

Site Status RECRUITING

University of Kansas Medical Center

Kansas City, Kansas, United States

Site Status RECRUITING

AMR Lexington

Lexington, Kentucky, United States

Site Status RECRUITING

Accurate Clinical Research Inc.

Lake Charles, Louisiana, United States

Site Status NOT_YET_RECRUITING

Mayo Clinic (Rochester)

Rochester, Minnesota, United States

Site Status RECRUITING

Las Vegas Clinical Trials

North Las Vegas, Nevada, United States

Site Status RECRUITING

Albuquerque Clinical Trials

Albuquerque, New Mexico, United States

Site Status RECRUITING

Center for Clinical Research

Winston-Salem, North Carolina, United States

Site Status NOT_YET_RECRUITING

Hightower Clinical

Oklahoma City, Oklahoma, United States

Site Status RECRUITING

University Orthopedics Center

Altoona, Pennsylvania, United States

Site Status RECRUITING

University Orthopedics Center

State College, Pennsylvania, United States

Site Status RECRUITING

AMR Knoxville

Knoxville, Tennessee, United States

Site Status NOT_YET_RECRUITING

Accurate Clinical Research, LLC

Baytown, Texas, United States

Site Status RECRUITING

Texas Orthopedic Specialist

Bedford, Texas, United States

Site Status RECRUITING

First Surgical Hospital

Bellaire, Texas, United States

Site Status RECRUITING

Accurate Clinical Research

Houston, Texas, United States

Site Status NOT_YET_RECRUITING

Endeavor Clinical Trials

San Antonio, Texas, United States

Site Status NOT_YET_RECRUITING

CenExel

Salt Lake City, Utah, United States

Site Status RECRUITING

Wasatch Clinical Research

Salt Lake City, Utah, United States

Site Status RECRUITING

Countries

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United States

Central Contacts

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Meghan Miller, MSS

Role: CONTACT

510-488-3832

Ernest R. Kitt, BS MS

Role: CONTACT

510-488-3832

Facility Contacts

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Hector Calderon, Site Coordinator

Role: primary

480-420-7681

Liane Encinas, Site Coordinator

Role: primary

520-612-7860

Jennifer Ruiz, Site Coordinator

Role: primary

469-210-8838

Swati Bhalla, Site Coordinator

Role: primary

626-869-5370

Farouk Awad, Site Coordinator

Role: primary

760-943-6700 ext. 162

Matt Campos, Site Coordinator

Role: primary

714-375-5970

Naomi Lupercio-Orozco Site Coordinator

Role: primary

951-924-6500

Daniela Castellanos, Site Coordinator

Role: primary

213-281-5146

Ligia Rosas, Site Coordinator

Role: primary

562-758-6600 ext. 5

Carlos Baez, Site Coordinator

Role: primary

407-500-5252

Damian Sosa, Site Coordinator

Role: primary

786-238-7099

Michelle De La Cruz, Site Coordinator

Role: primary

305-665-4818 ext. 212

Ariadna Zarzuela

Role: primary

786-577-5977 ext. 5001

Tanya Astorga, Site Coordinator

Role: primary

678-581-5252

Eric Halleran, Site Coordinator

Role: primary

816-943-0770

Jeannine Nilges, Site Coordinator

Role: primary

323-594-6184

Brittany Wilson, Site Coordinator

Role: primary

859-264-8999

Zachary Obmaces - Site Director

Role: primary

281-240-1045

Dave Sejal, Site Coordinator

Role: primary

507-266-9085

Brianna Catallini, Site Coordinator

Role: primary

702-637-3223

Valerie Escudero

Role: primary

505-224-7407 ext. 260

Jill Brewer, Site Director

Role: primary

336-403-3103

Megan Whitson, Site Coordinator

Role: primary

405-420-4689

Penny Adams, Site Coordinator

Role: primary

Jill Fremberg, Site Coordinator

Role: primary

814-272-3751

Elena Pintoc, Site Coordinator

Role: primary

865-410-8300

Mariela Martinez, Site Coordinator

Role: primary

832-705-7839

Amber Morgan, Site Coordinator

Role: primary

817-510-4022

Emilio Portillo, Site Coordinator

Role: primary

713-367-8548

Yanet Martinez, Site Coordinator

Role: primary

281-481-8557

Brian Purcell - Site Director

Role: primary

210-634-1253

Scott Zunkowski, Site Coordinator

Role: primary

801-261-2000 ext. 2322

Karen George, Site Coordinator

Role: primary

801-288-0607

Other Identifiers

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TPX-100-6

Identifier Type: -

Identifier Source: org_study_id

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