Comparison of Effectiveness of Disinfectants

NCT ID: NCT06811779

Last Updated: 2025-02-06

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

PHASE1

Total Enrollment

124 participants

Study Classification

INTERVENTIONAL

Study Start Date

2024-03-24

Study Completion Date

2024-06-25

Brief Summary

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This is a comparative experimental study to compare the efficacy of chemical disinfection agents , sodium hypochlorite and chlorhexidine gluconate in decontaminating gutta percha cones

Detailed Description

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The study design is a comparative study which was done to compare the efficacy of chemical disinfection agents on decontaminating the gutta percha cones .

In this study, 124 standardized size-80 gutta-percha (GP) cones (Dentsply, Germany) were used to assess the efficacy of chemical disinfectants.

The cones were first sterilized using ethylene oxide to ensure baseline sterility. Following sterilization, 124 cones were allocated into two experimental groups (n = 62 each) for contamination.

group A . gp were immersed in 20ml of Enterococcus faecalis solution group B . gp were immersed in 20 ml of human saliva in patients undergoing root canal therapy

samples were incubated at 37°C for 72 hours to ensure uniform and thorough contamination

after incubation period cones were dried and divide into subgroups based on disinfectant agent applied .

The cones were immersed in disinfectant agent for 1 min and 10 min . Cones were dried again and immersed in brain heard infusion broth samples were again incubated at 37°C for 72 hours The presence of bacterial growth was determined by assessing turbidity in the broth, indicating viable microorganisms.

Conditions

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Root Canal Infection

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

PARALLEL

Primary Study Purpose

PREVENTION

Blinding Strategy

TRIPLE

Participants Caregivers Investigators

Study Groups

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Naocl group

In this group, the Gp cones would be submerged in 5% naocl solution for 5 minutes and then tested for biofilm removal and eradication of inoculated microorganisms.

Group Type ACTIVE_COMPARATOR

Sodium Hypochlorite

Intervention Type DRUG

Sodium hypochlorite used in 5% concentration for disinfection

chlorhexidine group

In this group, the Gp cones would be submerged in 0.2% chlorhexidine solution for 5 minutes and then tested for biofilm removal and eradication of inoculated microorganisms.

Group Type ACTIVE_COMPARATOR

Chlorhexidine

Intervention Type DRUG

Chlorhexidine 0.2% concentration for disinfection

Interventions

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Sodium Hypochlorite

Sodium hypochlorite used in 5% concentration for disinfection

Intervention Type DRUG

Chlorhexidine

Chlorhexidine 0.2% concentration for disinfection

Intervention Type DRUG

Eligibility Criteria

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Inclusion Criteria

* healthy individual age between 20-40 patient needed root canal therapy

Exclusion Criteria

* patient not willing to participate in study participants with systemic disease patients with neuromuscular disorder
Minimum Eligible Age

20 Years

Maximum Eligible Age

40 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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Armed Forces Institute of Dentistry, Pakistan

OTHER

Sponsor Role lead

Responsible Party

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Hira Amjad

Dr. Hafiza Hira Amjad

Responsibility Role PRINCIPAL_INVESTIGATOR

Principal Investigators

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Hafiza Hira Amjad, BDS

Role: PRINCIPAL_INVESTIGATOR

Armed Forces Institute of Dentistry

Locations

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Armed Forces Institute of Dentistry

Rawalpindi, Punjab Province, Pakistan

Site Status

Countries

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Pakistan

Other Identifiers

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Disinfection

Identifier Type: -

Identifier Source: org_study_id

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