Potential Role of Sirtuins As Epigenetic Biomarkers of the Patient with Atherosclerosis or Calcific Aortic Stenosis

NCT ID: NCT06337227

Last Updated: 2025-03-11

Basic Information

• Recruitment Status: RECRUITING
• Total Enrollment: 3 participants
• Study Classification: OBSERVATIONAL
• Study Start Date: 2023-10-19
• Study Completion Date: 2026-10-31

Brief Summary

The project aims to shed light on the role of Sirtuins, enzymes belonging to the third class of histone deacetylases (class III) involved in epigenetic modifications (deacetylation), focusing mainly on Sirtuin 6 (SIRT6), as a regulator of atherosclerosis and severe aortic valve stenosis through molecular-based and epigenetic studies in human VSMC cells.

Conditions

Atherosclerosis and Calcific Aortic Stenosis with and Without Diabetes

Study Design

• Observational Model Type: COHORT
• Study Time Perspective: PROSPECTIVE

Study Groups

atherosclerosis and calcific aortic stenosis with diabetes patients

lncRNA signature analyses

Intervention Type: DIAGNOSTIC_TEST

Specific validation experiments will be performed by Real-Time PCR (C-reactive protein), Western Blot, flow cytometry and microscopy

atherosclerosis and calcific aortic stenosis without diabetes patients

lncRNA signature analyses

Intervention Type: DIAGNOSTIC_TEST

Specific validation experiments will be performed by Real-Time PCR (C-reactive protein), Western Blot, flow cytometry and microscopy

Patients without atherosclerosis undergoing surgical procedure for endocarditis

lncRNA signature analyses

Intervention Type: DIAGNOSTIC_TEST

Specific validation experiments will be performed by Real-Time PCR (C-reactive protein), Western Blot, flow cytometry and microscopy

Interventions

lncRNA signature analyses

Specific validation experiments will be performed by Real-Time PCR (C-reactive protein), Western Blot, flow cytometry and microscopy

Intervention Type: DIAGNOSTIC_TEST

Eligibility Criteria

Inclusion Criteria

• Patients with atherosclerosis or calcific aortic stenosis with and without diabetes older than 18 years of either sex;
• Patients without atherosclerosis undergoing surgical procedure for endocarditis;
• Patients who will have signed informed consent

Exclusion Criteria

• Patients for whom biological material cannot be recovered for study purposes;
• Patients who refuse to participate in the study

• Minimum Eligible Age: 18 Years
• Eligible Sex: ALL
• Accepts Healthy Volunteers: Yes

Sponsors

Federico II University

OTHER

Sponsor Role: collaborator

IRCCS SYNLAB SDN

OTHER

Sponsor Role: lead

Responsible Party

Responsibility Role: SPONSOR

Locations

Irccs Synlab Sdn

Naples, , Italy

Site Status: RECRUITING

Countries

Italy

Facility Contacts

Monica Franzese, PhD

Role: primary

direzionescientifica.irccssdn@synlab.it

+393334041413

Other Identifiers

2/22

Identifier Type: -

Identifier Source: org_study_id