The Tryptophan Requirement in Healthy Adults

NCT ID: NCT06283706

Last Updated: 2025-05-30

Basic Information

• Recruitment Status: RECRUITING
• Clinical Phase: NA
• Total Enrollment: 40 participants
• Study Classification: INTERVENTIONAL
• Study Start Date: 2024-06-01
• Study Completion Date: 2025-12-01

Brief Summary

The aging population is growing rapidly. For the first time ever, Canadian seniors outnumbered young adults in 2016. Aging is associated with many physical and metabolic deteriorations including the loss of muscle mass and strength, insulin resistance, increased inflammation and oxidative stress.

The muscles of our bodies are composed of proteins and proteins are made of small building blocks called amino acids. Our bodies have the ability to make some amino acids which are called the non-essential amino acids. However, there are certain amino acids that our body's need to make protein but can only be supplied from the foods that we consume. These are called essential amino acids. Tryptophan is one of the essential amino acids that the body needs. Tryptophan is required for protein synthesis and acts as a precursor for serotonin and nicotinic acid. Therefore, there may be a need for more threonine in the diet of older adults. However, the current requirement for threonine is based on studies conducted exclusively in young adults. Thus, the purpose of this study is to find out how much tryptophan older adults need.

Detailed Description

Tryptophan is an essential amino acid and must be obtained from the diet. It is required for protein synthesis and acts as a precursor for serotonin and nicotinic acid. It is of particular importance in the diet of people who receive a large percentage of their calories from cereal grains such as maize, as it is limiting in tryptophan. Therefore, accurate knowledge of tryptophan requirement is important for dietary planning particularly for older adults. In 1998, our lab determined the tryptophan requirement in young women to be 4.01 mg/kg/day, with a safe intake at 5 mg/kg/day, using the Indicator Amino Acid Oxidation method. Since then, the investigators have determined the tryptophan requirement and safe level in healthy school-age children to be 4.7 and 6 mg/kg/day. The investigators have recently found the requirements of essential amino acids such as leucine and total sulfur amino acids in older adults and have found to be higher than young adults and a difference between the sexes. Thus, there is a need to determine the threonine requirement in older adults.

Conditions

Healthy Aging

Study Design

• Allocation Method: NA
• Intervention Model: SINGLE_GROUP
• Primary Study Purpose: BASIC_SCIENCE
• Blinding Strategy: NONE

Study Groups

Tryptophan in Adults >60y

Tryptophan levels, up to 7 levels, will be tested in a random order in all subjects

Group Type: EXPERIMENTAL

Tryptophan

Intervention Type: OTHER

There are 7 different tryptophan test levels ranging from 0.5, 2, 4, 6, 8, 10, 12 mg.kg-1.day.1

Interventions

Tryptophan

There are 7 different tryptophan test levels ranging from 0.5, 2, 4, 6, 8, 10, 12 mg.kg-1.day.1

Intervention Type: OTHER

Eligibility Criteria

Inclusion Criteria

• Consent provided.
• Aged 60 to 90 years old.
• In good general health as evidenced by medical history, physical health and blood draw.
• Fasting blood glucose, hemoglobin A1c (HbA1c), urea, creatinine.
• Willingness to participate in the study.
• BMI <30 kg/m2.

Exclusion Criteria

• Presence of chronic disease and/or acute illness known to affect protein/amino acid metabolism (e.g. HIV, diabetes, taking medications known to affect protein/AA metabolism (e.g. steroids).
• Inability to tolerate the diet (i.e. allergy).
• Significant weight loss during the past month or consumption of weight reducing diets.
• Significant caffeine consumption (>2 cups per day).
• Significant consumption of alcohol (>1 drink per day i.e. 1 beer or ½ glass of wine).
• Unwilling to have blood drawn from a venous access or using a ventilated hood indirect calorimeter for the purposes of the study.

• Minimum Eligible Age: 60 Years
• Maximum Eligible Age: 90 Years
• Eligible Sex: ALL
• Accepts Healthy Volunteers: Yes

Sponsors

Canadian Institutes of Health Research (CIHR)

OTHER_GOV

Sponsor Role: collaborator

The Hospital for Sick Children

OTHER

Sponsor Role: lead

Responsible Party

Glenda Courtney-Martin

Senior Associate Scientist

Responsibility Role: PRINCIPAL_INVESTIGATOR

Principal Investigators

Glenda Courtney-Martin, PhD, RD

Role: PRINCIPAL_INVESTIGATOR

The Hospital for Sick Children

Locations

Hospital for Sick Children

Toronto, Ontario, Canada

Site Status: RECRUITING

Countries

Canada

Central Contacts

Zhiyao Wang, MSc

Role: CONTACT

zhiyao.wang@sickkids.ca

437 419 6224

Mahroukh Rafii, BSc

Role: CONTACT

mahroukh.rafii@sickkids.ca

647 215 1201

Facility Contacts

Glenda Courtney-Martin, PhD

Role: primary

glenda.courtney-martin@sickkids.ca

416-814-5744

Other Identifiers

1000081113

Identifier Type: -

Identifier Source: org_study_id