Temporal Variation of the Exposure to Pesticides in the Employees of the Limoges University Hospital

NCT ID: NCT06045234

Last Updated: 2025-01-17

Basic Information

• Recruitment Status: COMPLETED
• Clinical Phase: NA
• Total Enrollment: 300 participants
• Study Classification: INTERVENTIONAL
• Study Start Date: 2023-10-17
• Study Completion Date: 2024-08-02

Brief Summary

In a sample of 300 employees of the Limoges University Hospital, it is proposed to explore the intraindividual and interindividual variability of urinary concentrations of glyphosate and 320 other pesticides. The employees will fill a questionary in which will allow to explore the relationships between the concentrations, lifestyle and dietary habits.

Detailed Description

The general population is exposed to pesticides through the ingestion of food and water. The presence of pesticides in human urine has been demonstrated in numerous studies. Conventionally, biological monitoring studies use an undifferentiated urine sample or the first morning urine. The reliability of measuring a single-point urinary concentration to represent the profile of pesticide exposure in individuals over time is, however, questionable. Rare studies reporting intra-individual outcomes of pesticide exposure have been conducted in specific populations, such as children and pregnant women.

It is proposed to carry out a descriptive biomonitoring study of the urinary concentration of approximately 320 pesticides in a sample of the general population. Among these, the study will focus on glyphosate.

The originality of our study is:

• (i) Exploration of exposure to the main pesticides in a sample of 300 people by measuring urinary concentration by specific and sensitive methods of LC-MS/MS and GC-MS/MS; These 300 subjects will be hospital staff of the Limoges University Hospital.
• (ii) Exploration of the intra-individual variability of exposure by repeating measurements in urine samples taken over a 24-hour period and/or several times over the course of a week;
• (iii) Determination of the frequency of subjects who have potentially been exposed to doses higher than the acceptable daily intake (in particular for glyphosate) (iv) The association of these exposure values with data from a self-administered questionnaire in order to identify the determinants of this exposure
• (V) Estimating the predictability/reliability of a single urine sample to represent this exposure of individuals/populations over time.

Conditions

Toxicology

Study Design

• Allocation Method: NA
• Intervention Model: SINGLE_GROUP
• Primary Study Purpose: DIAGNOSTIC
• Blinding Strategy: NONE

Study Groups

Pesticide measurement

Group Type: EXPERIMENTAL

Urine sampling

Intervention Type: OTHER

Among the 300 people recruited:

• 300 will each provide 3 urine samples collected on 3 different days during a week (e.g. Monday, Wednesday and Friday)
• 50 will each provide 3 urine samples collected at sunrise, in the middle and then at the end of the day over a period of 24 hours, on 3 different days during a week (e.g. Monday, Wednesday and Friday).

Interventions

Urine sampling

Among the 300 people recruited:

• 300 will each provide 3 urine samples collected on 3 different days during a week (e.g. Monday, Wednesday and Friday)
• 50 will each provide 3 urine samples collected at sunrise, in the middle and then at the end of the day over a period of 24 hours, on 3 different days during a week (e.g. Monday, Wednesday and Friday).

Intervention Type: OTHER

Eligibility Criteria

Inclusion Criteria

• Adult 18 years and over.
• Employed by the University Hospital of Limoges.
• Having consented to participate in the study after being informed.

Exclusion Criteria

• Adult with a professional activity during which he is required to handle or come into contact with glyphosate

• Minimum Eligible Age: 18 Years
• Eligible Sex: ALL
• Accepts Healthy Volunteers: Yes

Sponsors

University Hospital, Limoges

OTHER

Sponsor Role: lead

Responsible Party

Responsibility Role: SPONSOR

Locations

CHU de Limoges

Limoges, , France

Countries

France

Other Identifiers

87RI23_0014 (VESTAL)

Identifier Type: -

Identifier Source: org_study_id