Spatially Transcriptomics Reveals Molecular Signatures in CRPS

NCT ID: NCT05986461

Last Updated: 2024-05-29

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

RECRUITING

Total Enrollment

10 participants

Study Classification

OBSERVATIONAL

Study Start Date

2023-07-18

Study Completion Date

2024-12-31

Brief Summary

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This observational study aims to understand phenotype-specific molecular signatures in patients with complex regional pain syndrome (CRPS).

Detailed Description

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Participants who meet the diagnostic criteria for CRPS, aged 19\~80 years, and agree to participate in this study will undergo a 4mm-sized skin punch biopsy in the involved limb, as well as the contralateral side (internal control).

Spatial transcriptomics will be used to identify immune cell signatures and differentially regulated genes (DEGs) in the epidermis, vasculature, sweat gland, and nerve plexus of the involved ipsilateral (vs contralateral) side of the patient's skin.

Conditions

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Complex Regional Pain Syndromes

Study Design

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Observational Model Type

CASE_ONLY

Study Time Perspective

CROSS_SECTIONAL

Eligibility Criteria

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Inclusion Criteria

* Meets the Budapest diagnostic criteria
* Consents to participation in this study
* Aged between 19 and 80 years old
* Symptoms and/or signs of sensory, vasomotor, and sudomotor changes

Exclusion Criteria

* Who do not agree to participate in this study
* Who has contraindications for skin punch biopsy (e.g. thrombocytopenia, taking anticoagulant drugs)
Minimum Eligible Age

19 Years

Maximum Eligible Age

80 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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Hanyang University

OTHER

Sponsor Role collaborator

Seoul National University

OTHER

Sponsor Role lead

Responsible Party

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Jeeyoun Moon

Professor

Responsibility Role PRINCIPAL_INVESTIGATOR

Principal Investigators

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Jee Youn Moon, MD, PhD

Role: PRINCIPAL_INVESTIGATOR

Seoul National University Hospital

Locations

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Seoul National University Hospital

Seoul, , South Korea

Site Status RECRUITING

Countries

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South Korea

Central Contacts

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Jee Youn Moon, MD, PhD

Role: CONTACT

82-10-5299-2036

Jeongsoo Kim, MD

Role: CONTACT

82-10-4734-6422

Facility Contacts

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Jee Youn Moon, M.D.

Role: primary

82-10-5299-2036

References

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Taylor SS, Noor N, Urits I, Paladini A, Sadhu MS, Gibb C, Carlson T, Myrcik D, Varrassi G, Viswanath O. Complex Regional Pain Syndrome: A Comprehensive Review. Pain Ther. 2021 Dec;10(2):875-892. doi: 10.1007/s40122-021-00279-4. Epub 2021 Jun 24.

Reference Type RESULT
PMID: 34165690 (View on PubMed)

Rao A, Barkley D, Franca GS, Yanai I. Exploring tissue architecture using spatial transcriptomics. Nature. 2021 Aug;596(7871):211-220. doi: 10.1038/s41586-021-03634-9. Epub 2021 Aug 11.

Reference Type RESULT
PMID: 34381231 (View on PubMed)

Birklein F, Drummond PD, Li W, Schlereth T, Albrecht N, Finch PM, Dawson LF, Clark JD, Kingery WS. Activation of cutaneous immune responses in complex regional pain syndrome. J Pain. 2014 May;15(5):485-95. doi: 10.1016/j.jpain.2014.01.490. Epub 2014 Jan 23.

Reference Type RESULT
PMID: 24462502 (View on PubMed)

Escolano-Lozano F, Gries E, Schlereth T, Dimova V, Baka P, Vlckova E, Konig S, Birklein F. Local and Systemic Expression Pattern of MMP-2 and MMP-9 in Complex Regional Pain Syndrome. J Pain. 2021 Oct;22(10):1294-1302. doi: 10.1016/j.jpain.2021.04.002. Epub 2021 Apr 20.

Reference Type RESULT
PMID: 33892152 (View on PubMed)

Stahl PL, Salmen F, Vickovic S, Lundmark A, Navarro JF, Magnusson J, Giacomello S, Asp M, Westholm JO, Huss M, Mollbrink A, Linnarsson S, Codeluppi S, Borg A, Ponten F, Costea PI, Sahlen P, Mulder J, Bergmann O, Lundeberg J, Frisen J. Visualization and analysis of gene expression in tissue sections by spatial transcriptomics. Science. 2016 Jul 1;353(6294):78-82. doi: 10.1126/science.aaf2403.

Reference Type RESULT
PMID: 27365449 (View on PubMed)

Ji AL, Rubin AJ, Thrane K, Jiang S, Reynolds DL, Meyers RM, Guo MG, George BM, Mollbrink A, Bergenstrahle J, Larsson L, Bai Y, Zhu B, Bhaduri A, Meyers JM, Rovira-Clave X, Hollmig ST, Aasi SZ, Nolan GP, Lundeberg J, Khavari PA. Multimodal Analysis of Composition and Spatial Architecture in Human Squamous Cell Carcinoma. Cell. 2020 Jul 23;182(2):497-514.e22. doi: 10.1016/j.cell.2020.05.039. Epub 2020 Jun 23.

Reference Type RESULT
PMID: 32579974 (View on PubMed)

Andronic D, Andronic O, Juengel A, Berli MC, Distler O, Brunner F. Skin biomarkers associated with complex regional pain syndrome (CRPS) Type I: a systematic review. Rheumatol Int. 2022 Jun;42(6):937-947. doi: 10.1007/s00296-021-05061-5. Epub 2022 Jan 8.

Reference Type RESULT
PMID: 34997300 (View on PubMed)

Other Identifiers

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2304-052-1421

Identifier Type: -

Identifier Source: org_study_id

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