Should Palatine Tonsils be Included in the Oropharyngeal Swab for SARS-CoV-2 Molecular Testing?

NCT ID: NCT05611203

Last Updated: 2023-01-11

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

UNKNOWN

Clinical Phase

NA

Total Enrollment

1000 participants

Study Classification

INTERVENTIONAL

Study Start Date

2022-11-10

Study Completion Date

2023-03-02

Brief Summary

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Citizens who undergo testing for COVID-19 at one of two Testcenters in Copenhagen Captial Region will be invited to participate in the study. The participants will be randomized to either an oropharyngeal swap including the palatine tonsils OR an oropharyngeal swap without the palatine tonsils for SARS-CoV-2 testing. The swabs will be sent to the clinical microbiology laboratory for SARS-CoV-2 detection using RT-PCR. The diagnostic accuracy and SARS-CoV-2 viral load will be compared. The participants will be asked to complete a questionnaire regarding their symptoms.

Detailed Description

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We will include citizens referred for outpatient testing for COVID-19 who will be offered to participante in the study on a volunteer basis. Participants are all volunteers, who are attending the test facilities to obtain a PCR test. Participants are required to provide oral and written informed consent to participate before entering the study. The inclusion criterion is 18 years or more of age. Exclusion criteria are neck breathers (tracheostomy/laryngectomy patients).

The participants will be randomized to either an oropharyngeal swap including the palatine tonsils OR an oropharyngeal swap without the palatine tonsils for SARS-CoV-2 testing.

The swabs will be sent to the clinical microbiology laboratory for SARS-CoV-2 detection using RT-PCR as usual. The diagnostic accuracy and SARS-CoV-2 viral load will be compared using independent t-test. The participants will be asked to complete a questionnaire regarding their symptoms and the number of sick days. All data will be documented on-site in a secure web database (REDCap).

The primary outcome will be reported as:

• SARS-CoV-2 RNA by RT-PCR test result (positive, negative, inconclusive)

The secondary outcome will be reported as:

* SARS-CoV-2 RT-PCR cycle threshold (Ct) value
* Test discomfort on a 11-point NRS-scale
* Development of COVID-19 disease after testing
* SARS-CoV-2 detection rate for each healthcare worker
* Mallampati Score of participants being tested

Conditions

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SARS-CoV Infection Coronavirus Corona Virus Infection

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

SINGLE_GROUP

Primary Study Purpose

DIAGNOSTIC

Blinding Strategy

NONE

Study Groups

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Oropharyngeal swab only including the posterior oropharyngeal wall

An Oropharyngeal swab is performed with the collection of specimens from the posterior oropharyngeal wall only

Group Type EXPERIMENTAL

An Oropharyngeal swab

Intervention Type PROCEDURE

An Oropharyngeal swab performed for diagnostic purposes

Oropharyngeal swab including the posterior oropharyngeal wall and both palatine tonsils

An Oropharyngeal swab is performed with the collection of specimens from the posterior oropharyngeal wall and both palatine tonsils

Group Type EXPERIMENTAL

An Oropharyngeal swab

Intervention Type PROCEDURE

An Oropharyngeal swab performed for diagnostic purposes

Interventions

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An Oropharyngeal swab

An Oropharyngeal swab performed for diagnostic purposes

Intervention Type PROCEDURE

Eligibility Criteria

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Inclusion Criteria

* citizens referred for outpatient testing for COVID-19 in Valby and Hillerød COVID-19 TestCenter.

Exclusion Criteria

* Age under 18 years
* neck breathers (tracheostomy/laryngectomy patients)
Minimum Eligible Age

18 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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Rigshospitalet, Denmark

OTHER

Sponsor Role lead

Responsible Party

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Tobias Todsen

Consultant, Associate Prof

Responsibility Role PRINCIPAL_INVESTIGATOR

Locations

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Hillerød Covid-19 Testcenter

Copenhagen, Hillerød, Denmark

Site Status RECRUITING

Valby

Copenhagen, , Denmark

Site Status RECRUITING

Countries

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Denmark

Central Contacts

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Tobias Todsen, MD, PhD

Role: CONTACT

+45 35466008

Facility Contacts

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Tobias Todsen, MD, PhD

Role: primary

Tobias Todsen, MD, PhD

Role: primary

References

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Hartvigsen B, Jakobsen KK, Benfield T, Gredal NT, Ersboll AK, Gronlund MW, Bundgaard H, Andersen MP, Steenhard N, von Buchwald C, Todsen T. Molecular Detection of SARS-CoV-2 From Throat Swabs Performed With or Without Specimen Collection From the Tonsils: Protocol for a Multicenter Randomized Controlled Trial. JMIR Res Protoc. 2024 Jun 12;13:e47446. doi: 10.2196/47446.

Reference Type DERIVED
PMID: 38865190 (View on PubMed)

Provided Documents

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Document Type: Study Protocol, Statistical Analysis Plan, and Informed Consent Form

View Document

Other Identifiers

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H-22022937

Identifier Type: -

Identifier Source: org_study_id

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