Nanopore Sequencing in Ophthalmology

NCT ID: NCT05372861

Last Updated: 2025-09-30

Basic Information

• Recruitment Status: RECRUITING
• Total Enrollment: 20 participants
• Study Classification: OBSERVATIONAL
• Study Start Date: 2022-04-22
• Study Completion Date: 2027-12-01

Brief Summary

In this pilot study, the feasibility of the Oxford Nanopore MinION to identify pathogens from vitrectomy samples in eyes with endophthalmitis shall be assessed. The MinION is a low cost commercially available device for DNA/ RNA analysis that, in studies, has been used for pathogen determination in various infectious diseases as well as for the genetic characterization of hematologic tumors.

Detailed Description

Vitreous samples and anterior chamber taps from eyes with endophthalmitis are taken during routine vitrectomy. The gathered samples are divided in half to undergo routine culture for microbial growth and nanopore sequencing with the MinION Mk1b, a commercially available device for DNA/RNA sequencing. Base reads from Nanopore sequencing are then compared with available libraries for pathogen identification. The Nanopore sequencing results for pathogens are compared versus the results of the microbiological culture of the same eye as the gold standard. Clinical features and visual function before and after vitrectomy are gathered from patient charts. Treatment is not altered due to results of the Nanopore sequencing.

Conditions

Endophthalmitis

Study Design

• Observational Model Type: CASE_ONLY
• Study Time Perspective: PROSPECTIVE

Eligibility Criteria

Inclusion Criteria

• Eyes of patients with endophthalmitis

Exclusion Criteria

• No informed consent possible
• Participation in other studies

• Minimum Eligible Age: 18 Years
• Maximum Eligible Age: 99 Years
• Eligible Sex: ALL
• Accepts Healthy Volunteers: No

Sponsors

Medical University of Graz

OTHER

Sponsor Role: lead

Responsible Party

Responsibility Role: SPONSOR

Locations

Universitäts Augenklinik Graz

Graz, Styria, Austria

Site Status: RECRUITING

Countries

Austria

Central Contacts

Michael Sommer, MD

Role: CONTACT

michael.sommer@medunigraz.at

004331638531033

Gerald Seidel, MD, PhD

Role: CONTACT

gerald.seidel@medunigraz.at

004331638580714

Facility Contacts

Michael Sommer, MD

Role: primary

michael.sommer@medunigraz.at

004331638531033

Gerald Seidel, MD, PhD

Role: backup

gerald.seidel@medunigraz.at

004331638580714

Other Identifiers

NSIO

Identifier Type: -

Identifier Source: org_study_id