Micro/Nanobubbles (MNBs) for Treatment of Acute and Chronic Wounds

NCT ID: NCT05169814

Last Updated: 2025-10-02

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

RECRUITING

Clinical Phase

EARLY_PHASE1

Total Enrollment

40 participants

Study Classification

INTERVENTIONAL

Study Start Date

2021-10-09

Study Completion Date

2027-06-01

Brief Summary

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The purpose of this study is to assess the safety and efficacy of Micro/nanobubbles (MNB's) for the healing of acute and chronic wounds.

Detailed Description

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Oxygen delivery is one of the primary factors in wound healing. Micro/nanobubbles (MNBs) can be used to increase the oxygen dissolved in solution and increase oxygen delivery to a wound. The purpose of this research study is to determine if MNBs applied to a wound improve wound healing.

After being informed about the study and potential risks, all patients will need to provide written informed consent before being included in the study. The characteristics of the wound will be assessed and measurements will be taken before and after treatment. Depending on the patient's wound type, the patient will be treated with MNBs in saline gauze which will be applied to the wound daily (for acute wounds), or MNBs in negative pressure wound therapy with instillation (NPWTi) (for chronic wounds) which will be applied to the wound continuously throughout the day with the wound evaluated and sponge replaced every 3-5 days. This is consistent with the current standard of wound care with gauze or NPWTi. Tissue oxygenation using infrared technology and wound healing will be measured and results collected for analysis.

Participation will last approximately 2-4 weeks or the duration of the inpatient admission. If discharge from the hospital is earlier than 2 weeks, the treatment will be discontinued and results will be submitted for analysis.

Conditions

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Open Wound Wound Heal

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

PARALLEL

This is a pilot study that consists of 4 arms:

* Acute wounds: 1 control arm; 1 experimental arm (treated with MNB irrigation)
* Chronic wounds: 1 control arm; 1 experimental arm (treated with Negative pressure wound therapy with MNB instillation)
Primary Study Purpose

TREATMENT

Blinding Strategy

DOUBLE

Participants Outcome Assessors
This is a double-blind, controlled study. Participants will be selected and matched based on similar wound profiles, pathology mechanisms, comorbidity profiles, and age. They will randomly be assigned to the experimental (MNB) or control (normal saline) group. The randomization ratio between both groups will be 1:1. Measurements of objective datapoints (e.g. StO2), cytokines, proteases, and pH will be taken by blinded research personnel.

Study Groups

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Acute Wounds - Control

This arm will include patients with acute wounds and will receive standard of care: irrigation with normal saline.

Group Type PLACEBO_COMPARATOR

0.9% Normal Saline - Irrigation

Intervention Type OTHER

A normal saline solution will be used as an irrigation solution to improve wound oxygenation in ischemic tissue (e.g. ischemic surgical tissue, traumatic wounds, and failing replants). The normal saline solution will be used in wet-to-dry wound care dressings with daily scheduled dressing changes.

Acute Wounds - Experimental

This arm will include patients with acute wounds and will receive experimental treatment: irrigation with micro/nanobubbles (MNB's) in normal saline.

Group Type EXPERIMENTAL

Micro/nanobubble (MNB) - Irrigation

Intervention Type DRUG

An MNB solution will be used as an irrigation solution to improve wound oxygenation in ischemic tissue (e.g. ischemic surgical tissue, traumatic wounds, and failing replants). The MNB solution will be used in wet-to-dry wound care dressings with daily scheduled dressing changes.

Chronic Wounds - Control

This arm will include patients with chronic wounds and will receive standard of care: negative pressure wound therapy with instillation (NPWTi) using normal saline.

Group Type PLACEBO_COMPARATOR

0.9% Normal Saline - Negative Pressure Wound Therapy with Instillation (NPWTi)

Intervention Type OTHER

NPWTi with normal saline will be applied to the wound with 20-minute instillation periods every 3 hours (standard instillation settings) with dressing changes every 3-5 days.

Chronic Wounds - Experimental

This arm will include patients with chronic wounds and will receive experimental treatment: negative pressure wound therapy with instillation (NPWTi) using micro/nanobubbles (MNB's) in normal saline.

Group Type EXPERIMENTAL

Micro/nanobubble (MNB) - Negative Pressure Wound Therapy with Instillation (NPWTi)

Intervention Type DRUG

NPWTi with MNB will be applied to the wound with 20-minute instillation periods every 3 hours (standard instillation settings) with dressing changes every 3-5 days.

Interventions

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Micro/nanobubble (MNB) - Irrigation

An MNB solution will be used as an irrigation solution to improve wound oxygenation in ischemic tissue (e.g. ischemic surgical tissue, traumatic wounds, and failing replants). The MNB solution will be used in wet-to-dry wound care dressings with daily scheduled dressing changes.

Intervention Type DRUG

0.9% Normal Saline - Irrigation

A normal saline solution will be used as an irrigation solution to improve wound oxygenation in ischemic tissue (e.g. ischemic surgical tissue, traumatic wounds, and failing replants). The normal saline solution will be used in wet-to-dry wound care dressings with daily scheduled dressing changes.

Intervention Type OTHER

Micro/nanobubble (MNB) - Negative Pressure Wound Therapy with Instillation (NPWTi)

NPWTi with MNB will be applied to the wound with 20-minute instillation periods every 3 hours (standard instillation settings) with dressing changes every 3-5 days.

Intervention Type DRUG

0.9% Normal Saline - Negative Pressure Wound Therapy with Instillation (NPWTi)

NPWTi with normal saline will be applied to the wound with 20-minute instillation periods every 3 hours (standard instillation settings) with dressing changes every 3-5 days.

Intervention Type OTHER

Eligibility Criteria

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Inclusion Criteria

* are above the age of 18.
* have traumatic, surgical, or chronic wounds.
* have radiotherapy related tissue injury.
* have thermal, chemical, and/or electrical burn injuries.
* have pressure ulcers, diabetic foot ulcers, venous ulcers, arterial ulcers, and/or neuropathic skin ulcers.
* have acute ischemic wounds

Exclusion Criteria

* have infected wounds.
* have wounds with exposed vital structures such as nerves, arteries, and/or veins.
* have wounds associated with malignancy.
Minimum Eligible Age

18 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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University of California, Irvine

OTHER

Sponsor Role lead

Responsible Party

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Raj Vyas

Lead Investigator

Responsibility Role PRINCIPAL_INVESTIGATOR

Principal Investigators

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Raj Vyas, MD

Role: PRINCIPAL_INVESTIGATOR

University of California, Irvine, Dept. of Plastic Surgery; Vice-Chairman

Locations

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UCI Medical Center

Orange, California, United States

Site Status RECRUITING

Countries

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United States

Central Contacts

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Leonardo Alaniz, BBA

Role: CONTACT

602-318-7118

Lohrasb R Sayadi, MD

Role: CONTACT

949-209-7267

Facility Contacts

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Leonardo Alaniz, MD

Role: primary

602-318-7118

Lohrasb R Sayadi, MD

Role: backup

(949) 209-7267

References

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Tsuge H. Micro- and Nanobubbles: Fundamentals and Applications. Boca Raton: CRC Press; 2014.

Reference Type BACKGROUND

Matiasek J, Djedovic G, Kiehlmann M, Verstappen R, Rieger UM. Negative pressure wound therapy with instillation: effects on healing of category 4 pressure ulcers. Plastic and Aesthetic Research. 2018;5.

Reference Type BACKGROUND

Sayadi LR, Banyard DA, Ziegler ME, Obagi Z, Prussak J, Klopfer MJ, Evans GR, Widgerow AD. Topical oxygen therapy & micro/nanobubbles: a new modality for tissue oxygen delivery. Int Wound J. 2018 Jun;15(3):363-374. doi: 10.1111/iwj.12873. Epub 2018 Jan 5.

Reference Type RESULT
PMID: 29314626 (View on PubMed)

Khan MS, Hwang J, Lee K, Choi Y, Kim K, Koo HJ, Hong JW, Choi J. Oxygen-Carrying Micro/Nanobubbles: Composition, Synthesis Techniques and Potential Prospects in Photo-Triggered Theranostics. Molecules. 2018 Aug 31;23(9):2210. doi: 10.3390/molecules23092210.

Reference Type RESULT
PMID: 30200336 (View on PubMed)

Lalezari S, Lee CJ, Borovikova AA, Banyard DA, Paydar KZ, Wirth GA, Widgerow AD. Deconstructing negative pressure wound therapy. Int Wound J. 2017 Aug;14(4):649-657. doi: 10.1111/iwj.12658. Epub 2016 Sep 29.

Reference Type RESULT
PMID: 27681204 (View on PubMed)

Back DA, Scheuermann-Poley C, Willy C. Recommendations on negative pressure wound therapy with instillation and antimicrobial solutions - when, where and how to use: what does the evidence show? Int Wound J. 2013 Dec;10 Suppl 1(Suppl 1):32-42. doi: 10.1111/iwj.12183.

Reference Type RESULT
PMID: 24251842 (View on PubMed)

Jarbrink K, Ni G, Sonnergren H, Schmidtchen A, Pang C, Bajpai R, Car J. The humanistic and economic burden of chronic wounds: a protocol for a systematic review. Syst Rev. 2017 Jan 24;6(1):15. doi: 10.1186/s13643-016-0400-8.

Reference Type RESULT
PMID: 28118847 (View on PubMed)

Provided Documents

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Document Type: Study Protocol and Statistical Analysis Plan

View Document

Document Type: Informed Consent Form

View Document

Other Identifiers

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154138

Identifier Type: OTHER

Identifier Source: secondary_id

5901

Identifier Type: -

Identifier Source: org_study_id

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