Sucrase-isomaltase Deficiency as a Cause of Irritable Bowel Syndrome

NCT ID: NCT05159115

Last Updated: 2022-02-02

Basic Information

• Recruitment Status: NOT_YET_RECRUITING
• Clinical Phase: NA
• Total Enrollment: 80 participants
• Study Classification: INTERVENTIONAL
• Study Start Date: 2022-03-14
• Study Completion Date: 2027-12-31

Brief Summary

Irritable bowel syndrome (IBS) is a functional disorder causing troublesome symptoms and reduced quality of life. It affects 10-20% of the population, hence creates large costs for society. About 30-40% of all IBS patients do not benefit from current treatment options. Sucrase-isomaltase (SI) deficiency is an unexplored condition, that may explain symptoms in IBS patients who experience no effect from today's treatments. Currently, a duodenal biopsy is the gold standard for the diagnosis of SI deficiency, however the condition is not well investigated. A 13C-labelled breath test holds promise as a non-invasive alternative, but it has not previously been validated.

This project will address the knowledge gap related to a possible association between SI deficiency and IBS by addressing two research questions that have never been answered before. We aim to:

1. Validate the 13C-labelled breath test as a diagnostic tool by assessing the strength of the association between the breath test and SI activity measured in duodenal biopsies

2. Use the 13C-labelled breath test in a randomized dietary crossover trial comparing a starch and sucrose reduced diet (SSRD) with the standard low-FODMAP diet in IBS patients, to evaluate whether SI activity is associated with dietary changes according to symptom severity and gut microbiota composition

Detailed Description

The projects includes two studies:

Study 1: Validation of the 13C-labelled breath test to diagnose sucrase-isomaltase deficiency

Background: In order to validate the 13C-sucrose breath test to diagnose SI deficiency, the test result must be compared to the "gold standard" method for diagnosis; i.e. measurements of enzyme activity from intestinal biopsies by "The Dahlquist Method", and a reference material must be established.

Objective: To compare results from the 13C-labelled breath test to enzyme activity measured in biopsies collected from the proximal small intestine in a limited patient group referred for a gastroscopic examination.

Design: A cross-sectional study.

Primary endpoint: SI activity as measured with a breath test and enzyme activity with assay of biopsy material.

Recruitment and patient characteristics: Patients referred for gastroscopic examination with duodenal biopsies and with suspected GI disorder, will be included consecutively.

Sample size: We aim to include 40 patients. No studies validating breath test results in our patient group are available. However, based on preliminary results suggesting that 35% of IBS patients have SI deficiency,13 we are 95% likely to find between 8 and 21 patients with SI deficiency when examining 40 patients. Assuming 80% concordance between the two methods to (correct proportion of successes), we would need 12 positive cases. Thus, if 40 individuals are included, the study is sufficiently powered (alpha=5% and beta=20%, using McNemar's test of concordance).

Study 2: Sucrase-isomaltase deficiency as a cause of symptoms in patients with irritable bowel syndrome

Objectives: To examine the effect of a 4-week SSRD on GI- and extraintestinal symptoms, gut microbiota composition and fecal fermentation in patients with IBS (with and without SI deficiency), and compare the SSRD with a 4-week low-FODMAP diet to investigate whether the patients with a breath test result indicating SI deficiency respond better to the SSRD than the patients with normal SI activity. Gut microbiota have been suggested to have a central role in IBS etiology, hence evaluation of gut microbiota composition and fecal fermentation will be included to increase the knowledge regarding the effects of dietary change on gut microbiota composition and activity related to SI deficiency.

Design: A randomized, open clinical crossover trial with a dietary intervention in a group of IBS patients, lasting for 4+4 weeks (SSRD vs. low-FODMAP) with a 1-week wash-out period in between. Breath tests will be taken at inclusion, but the results will be "blinded", e.g. not available for anyone conducting the trial before end of the study. A SSRD will be compared to the low-FODMAP diet. All participants will be given dietary advice from a registered clinical dietitian. Briefly, all forms of sucrose-containing foods (e.g. sweets, jam, and cakes) should be avoided, and foods rich in starch should be reduced on the SSRD.

Primary endpoint: Symptom severity by IBS-Symptom Severity Scale (IBS-SSS).

Secondary endpoints: Gut microbiota composition, fecal fermentation measured by short chain fatty acids (SCFAs) and assessment of quality of life by the Patient Reported Outcome Measurement Information System (PROMIS-29).

Recruitment and patient characteristics: IBS patients referred to the outpatient clinic at the Department of Gastroenterology at Lovisenberg Diaconal Hospital for dietary guidance by a registered dietitian will be consecutively included.

Sample size: The primary end point is change in IBS symptom severity (IBS-SSS) at the end of the treatment period relative to baseline, and the proportion of responders to the dietary intervention is based on the recommended cut-off of a reduction (ie, improvement) in total IBS-SSS score of 50 points, which is considered to be clinically meaningful improvement. To plan our sample size, we performed a power calculation based on the ability to detect a difference between the two diets in reduction of IBS-SSS score of at least 50 points with 80% power, assuming an SD of 100. The calculation indicated that we would need 64 patients. To allow for 15-20% drop-out, we aim to include 80 patients.

Conditions

Irritable Bowel Syndrome; Sucrose Intolerance Due to Sucrase-Isomaltase Deficiency; Carbohydrate; Malabsorption; Sucrase Isomaltase Deficiency; Functional Gastrointestinal Disorders

Study Design

• Allocation Method: RANDOMIZED
• Intervention Model: CROSSOVER
• Primary Study Purpose: TREATMENT
• Blinding Strategy: NONE

Study Groups

Low-FODMAP diet

Patients with IBS on a 4-week low-FODMAP diet.

Group Type: ACTIVE_COMPARATOR

Low-FODMAP diet

Intervention Type: OTHER

4 weeks of a diet low in fermentable oligosaccharides, disaccharides, monosaccharides and polyols (FODMAPs)

Starch- and Sucrose Reduced Diet

Patients with IBS on a s 4-week Starch- and Sucrose Reduced Diet (SSRD).

Group Type: EXPERIMENTAL

Starch- and Sucrose Reduced Diet (SSRD)

Intervention Type: OTHER

4 weeks of a diet low in starch and sucrose

Interventions

Low-FODMAP diet

4 weeks of a diet low in fermentable oligosaccharides, disaccharides, monosaccharides and polyols (FODMAPs)

Intervention Type: OTHER

Starch- and Sucrose Reduced Diet (SSRD)

4 weeks of a diet low in starch and sucrose

Intervention Type: OTHER

Eligibility Criteria

Inclusion Criteria

• Signed informed consent
• BMI 18-30 kg/m2
• Referred for gastroscopic examination with suspected GI disorder

• Signed informed consent
• BMI 18-30 kg/m2
• IBS diagnosis according to Rome IV criteria

Exclusion Criteria

• Unwilling or not capable of signing the informed consent

Sucrase-isomaltase deficiency as a cause of symptoms in patients with irritable bowel syndrome (n=80)

• Inflammatory bowel disease, celiac disease or diabetes mellitus
• Chronic immune diseases affecting the GI-system
• Unwilling/unable to maintain a stable diet throughout the study period
• Use of antibiotic treatment for the last 4 weeks
• Currently on a restrictive diet

• Minimum Eligible Age: 18 Years
• Maximum Eligible Age: 90 Years
• Eligible Sex: ALL
• Accepts Healthy Volunteers: No

Sponsors

University of Bergen

OTHER

Sponsor Role: collaborator

Lovisenberg Diakonale Hospital

OTHER

Sponsor Role: lead

Responsible Party

Responsibility Role: SPONSOR

Central Contacts

Jørgen Valeur, PhD

Role: CONTACT

jorgen.valeur@lds.no

0047 23225140

Other Identifiers

MED 358

Identifier Type: -

Identifier Source: org_study_id