Oxidative Stress and L-CBMN Cytome Assay in Obese After 3 Weeks VLCD

NCT ID: NCT05055154

Last Updated: 2021-09-24

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

NA

Total Enrollment

26 participants

Study Classification

INTERVENTIONAL

Study Start Date

2019-06-14

Study Completion Date

2020-11-03

Brief Summary

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Obesity leads to physiological imbalance resulting in hyperglycemia, dyslipidaemia and inflammation and can generate systematic oxidative stress through multiple biochemical mechanisms. Oxidative stress (OS) can induce DNA damage and inhibit DNA repair mechanisms. Very low calorie diet (VLCD) have rapid positive effect on weight loss, glucose homeostasis, insulin resistance, inflammation and OS. The aim of this study is to determine the effect of a three-week VLCD on anthropometric, biochemical and genomic parameters in individuals with BMI ≥ 35kg/m2.

Detailed Description

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Obesity is a complex chronic multifactorial disease associated with concomitant or increased risk for chronic inflammation, insulin resistance, dyslipidemia, oxidative stress, type 2 diabetes, cardiovascular disease, stroke and multiple cancer types. Oxidative stress (OS) can cause permanent DNA damage which could be detected with lymphocytes cytokinesis-block micronucleus (L-CBMN) cytome assay. Weight loss and improvement of dietary habits in people with obesity can affect genome stability and have beneficial effects on insulin sensitivity, inflammation and OS. Effects of very low calorie diet (VLCD) on DNA damage are scarce. The aim of this study is to determine the effect of a three-week VLCD used in Special Hospital for extended treatment of Duga Resa in patients with BMI ≥ 35kg/m2 on permanent DNA damage, lipid profile, insulin resistance, inflammation and anthropometric parameters.

Conditions

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Obesity Weight Loss DNA Damage

Study Design

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Allocation Method

NA

Intervention Model

SINGLE_GROUP

Primary Study Purpose

TREATMENT

Blinding Strategy

NONE

Study Groups

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Very low calorie diet

Use of very low calorie diet prepared in the hospital

Group Type EXPERIMENTAL

Very low calorie diet

Intervention Type DEVICE

In hospital patients will eat prepared diet with 567 kcal a day during 3 weeks

Interventions

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Very low calorie diet

In hospital patients will eat prepared diet with 567 kcal a day during 3 weeks

Intervention Type DEVICE

Eligibility Criteria

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Inclusion Criteria

* body mass index ≥ 35 kg/m2

Exclusion Criteria

* pregnancy
* actual tumor diseases
* recent diagnostic or treatment exposures to ionizing radiation in the period of one year
* individuals not willing to stay 3 weeks under supervision under full 24 h surveillance from the medical stuff
Minimum Eligible Age

18 Years

Maximum Eligible Age

70 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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Institute for Medical Research and Occupational Health

UNKNOWN

Sponsor Role collaborator

University of Zagreb

OTHER

Sponsor Role collaborator

Special Hospital for Extended Treatment of Duga Resa

OTHER_GOV

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Mirta Milic, PhD

Role: PRINCIPAL_INVESTIGATOR

Institute for Medical Research and Occupational Health

Dragan Bozcevic, Doctor

Role: PRINCIPAL_INVESTIGATOR

Special Hospital for Extended Treatment of Duga Resa

Ana-Marija Domijan, Prof.

Role: PRINCIPAL_INVESTIGATOR

University of Zagreb

Locations

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Special Hospital for Extended Treatment of Duga Resa

Duga Resa, , Croatia

Site Status

Countries

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Croatia

References

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Reference Type BACKGROUND
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Reference Type BACKGROUND
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Reference Type BACKGROUND
PMID: 15599400 (View on PubMed)

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Reference Type BACKGROUND
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Reference Type BACKGROUND
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Reference Type BACKGROUND
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Reference Type BACKGROUND
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Reference Type BACKGROUND
PMID: 25308439 (View on PubMed)

Other Identifiers

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OSMN35aVLCD-26

Identifier Type: -

Identifier Source: org_study_id

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