Effectiveness of Folic Acid Supplementation in Acute Watery Diarrhea Among Children Under 5 Years of Age

NCT ID: NCT04782037

Last Updated: 2022-05-19

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

UNKNOWN

Clinical Phase

PHASE4

Total Enrollment

324 participants

Study Classification

INTERVENTIONAL

Study Start Date

2020-10-21

Study Completion Date

2022-07-01

Brief Summary

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Folic acid is a palatable and easily available drug with no serious adverse effects. L-methylfolate is its biologically activated form and is preferred because of its direct availability for certain metabolic processes. As there is no specific and safe drug available that may help in decreasing the duration of diarrhea, purging rate or consistency of stools; and having known the theoretical benefits of folic acid in this regard, it would be appropriate to assess the effectiveness of L-methylfolate among children with diarrhea.

Detailed Description

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After approval from the Institutional Review Board of King Edward Medical University, Mayo Hospital, Lahore, all children diagnosed with acute watery diarrhea as per operational definition fulfilling the inclusion criteria, presenting to the Department of Pediatrics, Mayo Hospital, will be enrolled in this study.

Informed written consent will be taken from the parents/guardians of all participants. A detailed history and examination of each subject would be done by an investigator at presentation.

Patients will be randomized in two groups - A and B by lottery method. There will be 162 patients in each group. The treatment regimen that includes breast feeding, nutritional advice, rehydration plan; ORT by low osmolar ORS or intravenous rehydration (if required) and zinc sulphate will be given according to the WHO guidelines to all patients of both groups. Children under 1 year of age in group A will receive 3 drops (90mcg) of L-methylfolate calcium) while those older than 1 year of age will be given 5 drops (150mcg). Subjects in Group B will receive equal amount of distilled water as placebo (i.e. 3 drops to \<1yr age and 5 drops to \>1 yr. Frequency and grades of loose stools in both groups will be assessed daily and written down on a predesigned proforma.

Conditions

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Diarrhoea;Acute

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

FACTORIAL

Primary Study Purpose

TREATMENT

Blinding Strategy

SINGLE

Participants

Study Groups

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L-methylfolate supplementation

Children under 1 year of age in group A will receive 3 drops (90mcg) of L-methylfolate calcium) while those older than 1 year of age will be given 5 drops (150mcg) daily fior 5 days

Group Type EXPERIMENTAL

L-Methylfolate Calcium

Intervention Type DRUG

Folic acid is one of the water-soluble B vitamins, which is synthetically-produced and found in fortified foods and supplements. It is essential for the synthesis and repair of DNA and RNA and metabolism of amino acids which are required for cell division. There is damage to the intestinal mucosa in most diarrhea cases; therefore, the role of folic acid has been studied as adjuvant therapy for diarrhea since folic acid plays an important role in the synthesis of DNA especially in rapidly regenerating cells.

distilled water

Subjects in Group B will receive equal amount of distilled water as placebo (i.e. 3 drops to \<1yr age and 5 drops to \>1 yr.

Group Type PLACEBO_COMPARATOR

L-Methylfolate Calcium

Intervention Type DRUG

Folic acid is one of the water-soluble B vitamins, which is synthetically-produced and found in fortified foods and supplements. It is essential for the synthesis and repair of DNA and RNA and metabolism of amino acids which are required for cell division. There is damage to the intestinal mucosa in most diarrhea cases; therefore, the role of folic acid has been studied as adjuvant therapy for diarrhea since folic acid plays an important role in the synthesis of DNA especially in rapidly regenerating cells.

Interventions

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L-Methylfolate Calcium

Folic acid is one of the water-soluble B vitamins, which is synthetically-produced and found in fortified foods and supplements. It is essential for the synthesis and repair of DNA and RNA and metabolism of amino acids which are required for cell division. There is damage to the intestinal mucosa in most diarrhea cases; therefore, the role of folic acid has been studied as adjuvant therapy for diarrhea since folic acid plays an important role in the synthesis of DNA especially in rapidly regenerating cells.

Intervention Type DRUG

Eligibility Criteria

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Inclusion Criteria

* Children aged 2months- 5 years
* Both genders
* Acute watery diarrhea as per operational definition
* Diarrhea of less than 7-day duration

Exclusion Criteria

* Severely malnourished children \<3 SD
* Bloody diarrhea
* Prolonged (7-14 days duration), persistent (\>14 days duration) or chronic diarrhea (\>4weeks duration)
* Those who received folic acid within the last 14 days
* Hospital-acquired diarrhea (diarrhea occurring after 48hrs of stay in hospital)
* Antibiotic-associated diarrhea.
* Presence of other co morbid conditions like pneumonia, sepsis, meningitis, etc.
Minimum Eligible Age

2 Months

Maximum Eligible Age

5 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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King Edward Medical University

OTHER

Sponsor Role lead

Responsible Party

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rafia Jamil

Post Graduate Resident (MD Pediatric Medicine)

Responsibility Role PRINCIPAL_INVESTIGATOR

Principal Investigators

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MUHAMMAD Haroon HAMID, MBBS,FCPS

Role: PRINCIPAL_INVESTIGATOR

CHAIRMAN PEDIATRIC MEDICINE, MAYO HOSPITAL

Sadia shabir, MBBS, FCPS

Role: PRINCIPAL_INVESTIGATOR

ASSISTANT PROFESSOR, PEDIATRIC MEDICINE UNIT 1, MAYO HOSPITAL

Locations

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Mayo hospital

Lahore, Punjab Province, Pakistan

Site Status RECRUITING

Countries

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Pakistan

Central Contacts

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RAFIA M JAMIL, MBBS

Role: CONTACT

0092-330-4513250

MUHAMMAD HAROON Haroon HAMID, MBBS,FCPS

Role: CONTACT

0092-300-8880916

Facility Contacts

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Rafia Jamil hospital, MBBS

Role: primary

0092-330-4513250

Sadia Shabir, MBBS,FCPS

Role: backup

0092-345-4736393

References

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Kotloff KL. The Burden and Etiology of Diarrheal Illness in Developing Countries. Pediatr Clin North Am. 2017 Aug;64(4):799-814. doi: 10.1016/j.pcl.2017.03.006.

Reference Type BACKGROUND
PMID: 28734511 (View on PubMed)

GBD Diarrhoeal Diseases Collaborators. Estimates of global, regional, and national morbidity, mortality, and aetiologies of diarrhoeal diseases: a systematic analysis for the Global Burden of Disease Study 2015. Lancet Infect Dis. 2017 Sep;17(9):909-948. doi: 10.1016/S1473-3099(17)30276-1. Epub 2017 Jun 1.

Reference Type BACKGROUND
PMID: 28579426 (View on PubMed)

Walker CLF, Rudan I, Liu L, Nair H, Theodoratou E, Bhutta ZA, O'Brien KL, Campbell H, Black RE. Global burden of childhood pneumonia and diarrhoea. Lancet. 2013 Apr 20;381(9875):1405-1416. doi: 10.1016/S0140-6736(13)60222-6. Epub 2013 Apr 12.

Reference Type BACKGROUND
PMID: 23582727 (View on PubMed)

Ahs JW, Tao W, Löfgren J, Forsberg BC. Diarrheal diseases in low-and middle-income countries: incidence, prevention, and management. Open Infect Dis J. 2010; 4(1):113-24.

Reference Type BACKGROUND

Crider KS, Yang TP, Berry RJ, Bailey LB. Folate and DNA methylation: a review of molecular mechanisms and the evidence for folate's role. Adv Nutr. 2012 Jan;3(1):21-38. doi: 10.3945/an.111.000992. Epub 2012 Jan 5.

Reference Type BACKGROUND
PMID: 22332098 (View on PubMed)

Kadhum RJ, Alghizzi EJ. The effect of oral folic acid supplementation on the course of acute infantile diarrhea. J Kerbala Univ. 2010; 8(4):177-82.

Reference Type BACKGROUND

Amelia A, Sinohaji AB, Supriatmo. Folic acid and acute diarrhea in children. Pediatr Indones. 2014; 54(5):273-9

Reference Type BACKGROUND

Niederberger KE, Dahms I, Broschard TH, Boehni R, Moser R. Safety evaluation of calcium L-methylfolate. Toxicol Rep. 2019 Sep 26;6:1018-1030. doi: 10.1016/j.toxrep.2019.09.012. eCollection 2019.

Reference Type BACKGROUND
PMID: 31673504 (View on PubMed)

Other Identifiers

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321/RC/KEMU

Identifier Type: -

Identifier Source: org_study_id

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