Role of the Gut Sweet Taste Receptor in the Secretion of Glucagon-like Peptide-1 and Peptide YY

NCT ID: NCT01304823

Last Updated: 2015-07-16

Basic Information

• Recruitment Status: COMPLETED
• Clinical Phase: PHASE1
• Total Enrollment: 10 participants
• Study Classification: INTERVENTIONAL
• Study Start Date: 2010-06-30
• Study Completion Date: 2011-01-31

Brief Summary

The purpose of this study is to determine the functional significance of sweet taste receptors in the secretion of GI satiation peptides by using a specific sweet taste receptor antagonist to block sweet taste perception in the gastrointestinal tract.

Detailed Description

There is strong evidence that taste signaling mechanisms identified in the oral epithelium also operate in the gut. It is suggested that open-type enteroendocrine cells directly sense nutrient via alpha-gustducin coupled taste receptors to modulate the secretion of glucagon like peptide-1 (GLP-1) and peptide YY (PYY). Several nutrient responsive G-protein coupled receptors have been identified in the human gut, including the sweet taste responsive T1R2/T1R3 heterodimer, the amino acid/umami responsive T1R1/T1R3 as well as GPR120 for unsaturated long-chain free fatty acids.The functional significance of sweet taste receptors in the secretion of GLP-1 and PYY will be determined by intraduodenal perfusion of glucose (I) or a mixed liquid meal (II) with or without lactisole (450 ppm) in a double blind, 5-way crossover trial including 10 healthy subjects.

Conditions

Taste Disorder, Primary, Sweet

Study Design

• Allocation Method: RANDOMIZED
• Intervention Model: CROSSOVER
• Primary Study Purpose: BASIC_SCIENCE
• Blinding Strategy: QUADRUPLE

Study Groups

glucose

intraduodenal perfusion of glucose (29.3 g glucose per 100 mL; rate: 2.5 mL/min for 180 min; caloric load: 3.0 kcal/min)

Group Type: ACTIVE_COMPARATOR

lactisole

Intervention Type: DIETARY_SUPPLEMENT

flavoring agent/sweet taste antagonist

glucose + lactisole

intraduodenal perfusion of glucose (29.3 g glucose per 100 mL; rate: 2.5 mL/min for 180 min; caloric load: 3.0 kcal/min) + 450 ppm lactisole

Group Type: ACTIVE_COMPARATOR

lactisole

Intervention Type: DIETARY_SUPPLEMENT

flavoring agent/sweet taste antagonist

mixed liquid meal

intraduodenal perfusion of a mixed liquid meal (20.20 g carbohydrate, 4.92 g fat and 6.25 g protein per 100 mL; rate: 2.5 mL/min for 180 min; caloric load: 3.0 kcal/min)

Group Type: ACTIVE_COMPARATOR

mixed liquid meal

Intervention Type: OTHER

Ensure Plus

mixed liquid meal + lactisole

intraduodenal perfusion of a mixed liquid meal (20.20 g carbohydrate, 4.92 g fat and 6.25 g protein per 100 mL; rate: 2.5 mL/min for 180 min; caloric load: 3.0 kcal/min) + 450 ppm lactisole

Group Type: ACTIVE_COMPARATOR

mixed liquid meal

Intervention Type: OTHER

Ensure Plus

saline + lactisole

saline (0.9 %; rate: 2.5 mL/min for 180 min) + 450 ppm lactisole

Group Type: PLACEBO_COMPARATOR

lactisole

Intervention Type: DIETARY_SUPPLEMENT

flavoring agent/sweet taste antagonist

Interventions

lactisole

flavoring agent/sweet taste antagonist

Intervention Type: DIETARY_SUPPLEMENT

mixed liquid meal

Ensure Plus

Intervention Type: OTHER

Other Intervention Names

Dietary supplement; Dietary supplement

Eligibility Criteria

Inclusion Criteria

• Healthy subjects
• BMI of 19.0-24.5
• Age 18-40
• stable body weight for at least 3 month

Exclusion Criteria

• smoking
• substance abuse
• regular intake of medication
• medical or psychiatric illness
• gastrointestinal disorders or food allergies

• Minimum Eligible Age: 18 Years
• Maximum Eligible Age: 40 Years
• Eligible Sex: MALE
• Accepts Healthy Volunteers: Yes

Sponsors

University Hospital, Basel, Switzerland

OTHER

Sponsor Role: lead

Responsible Party

Responsibility Role: SPONSOR

Principal Investigators

Christoph Beglinger, MD

Role: PRINCIPAL_INVESTIGATOR

Phase 1 Research Unit, University Hospital Basel

Locations

University Hospital Basel, Phase 1 Research Unit

Basel, , Switzerland

Countries

Switzerland

References

Steinert RE, Meyer-Gerspach AC, Beglinger C. The role of the stomach in the control of appetite and the secretion of satiation peptides. Am J Physiol Endocrinol Metab. 2012 Mar 15;302(6):E666-73. doi: 10.1152/ajpendo.00457.2011. Epub 2012 Jan 3.

Reference Type: RESULT

PMID: 22215654 (View on PubMed)

Steinert RE, Gerspach AC, Gutmann H, Asarian L, Drewe J, Beglinger C. The functional involvement of gut-expressed sweet taste receptors in glucose-stimulated secretion of glucagon-like peptide-1 (GLP-1) and peptide YY (PYY). Clin Nutr. 2011 Aug;30(4):524-32. doi: 10.1016/j.clnu.2011.01.007. Epub 2011 Feb 15.

Reference Type: RESULT

PMID: 21324568 (View on PubMed)

Other Identifiers

EKBB 69/04.03

Identifier Type: -

Identifier Source: org_study_id