COVID-19 Longitudinal Biomarkers in Lung Injury

NCT ID: NCT04747782

Last Updated: 2021-02-10

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

UNKNOWN

Total Enrollment

200 participants

Study Classification

OBSERVATIONAL

Study Start Date

2020-03-23

Study Completion Date

2021-12-31

Brief Summary

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Profile known and novel biomarkers in blood in COVID19 patients to characterize the host response to SARS-CoV-2 over time and in response to treatment.

The investigators aim to:

* Better understand the disease. The investigators will achieve this by characterizing the biology of COVID-19 infection and the pathophysiology of the host response using clinical data together with cellular and molecular measurements over the course of the disease. This will allow better insights for the discovery and development of novel therapeutics.
* Understand why different patients have different phenotypes and disease presentations over time. The investigators will achieve this by analyzing for patient subgroups. This will allow targeted patient stratification and better matching of resources.
* Understand how patients are responding to the different medications being tested in clinical trials. The investigators will achieve that by co-enrolling with therapeutic trials. This will allow an understanding of the biological effects of these interventions.

Study Design: Observational adaptive study of a translational nature, combining clinical data and basic science investigations in blood samples in the same patients, longitudinally, with serial interim analyses.

Primary outcomes: 90 day ICU mortality. Secondary outcomes: measures of ICU utilization and disease severity, and 90 day in-hospital mortality.

The study ends after 3 months from admission to the ICU, hospital discharge or death.

Location: St. Michael's Hospital (Unity Health Toronto), an academic center in downtown Toronto affiliated with the University of Toronto.

The investigators will collect: A) Detailed clinical data including investigations, mechanical ventilation and cardiovascular parameters. B) Blood samples for state-of-the-art multi-omics biomarker discovery and development: cytokines, anti-COVID19 antibodies, autoimmune serology, metabolomics, transcriptomics, epigenomics, deep immune phenotyping, viral loads. For those patients who die with COVID19 The investigators will perform bedside post-mortem biopsies of lung, heart, kidney and muscle.

Sampling times: From admission to the maximal severity phase through convalescence, in order to capture the evolution and dynamics of the disease and the recovery process: days 0,1, 3, 5, 7, 10, 15 and 22, and then every 2 weeks until the end of the study (3 months from admission to the ICU, hospital discharge or death).

Detailed Description

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Conditions

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Covid19 Respiratory Disease Critical Illness

Study Design

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Observational Model Type

COHORT

Study Time Perspective

PROSPECTIVE

Study Groups

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COVID positive

Patients admitted to the ICU with respiratory distress found to be COVID19 positive.

no interventions performed. Just collecting data, blood samples and post-mortem biopsies.

Intervention Type OTHER

no interventions performed. Just collecting data, blood samples and post-mortem biopsies.

COVID negative

Patients admitted to the ICU with respiratory distress found to be COVID19 negative

no interventions performed. Just collecting data, blood samples and post-mortem biopsies.

Intervention Type OTHER

no interventions performed. Just collecting data, blood samples and post-mortem biopsies.

COVID positive delayed

Patients admitted to the ICU for an indication other than respiratory distress, found to be COVID19 positive.

no interventions performed. Just collecting data, blood samples and post-mortem biopsies.

Intervention Type OTHER

no interventions performed. Just collecting data, blood samples and post-mortem biopsies.

never-ICU

Patients admitted to the internal medicine ward with respiratory distress found to be COVID19 positive.

no interventions performed. Just collecting data, blood samples and post-mortem biopsies.

Intervention Type OTHER

no interventions performed. Just collecting data, blood samples and post-mortem biopsies.

Interventions

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no interventions performed. Just collecting data, blood samples and post-mortem biopsies.

no interventions performed. Just collecting data, blood samples and post-mortem biopsies.

Intervention Type OTHER

Eligibility Criteria

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Inclusion Criteria

* Secondary Cohort: Patients admitted to the ICU for any reason who are COVID19 positive, regardless of admission date or when they are found to be COVID19 positive. This population will be called "ICU Positive - delayed"
* Biological controls in the ICU: Any patients from the primary cohort who are found to be COVID19 negative. This population will be called "ICU negative".
* Biological controls outside the ICU: Patients admitted to the floor with respiratory deterioration suspected or confirmed to be due to SARS-CoV-2, or that develop such respiratory deterioration within 15 days of admission (i.e. become "persons under investigation"). Suspicion will be based on the clinical criteria in place at SMH, with the threshold for inclusion being a suspicion high enough to use PPE until confirmatory testing results are obtained. Only patients found to be COVID19 positive will stay in this cohort. If these patients require ICU admission within 15 days, they will cross-over to the "ICU positive" cohort and count towards that cohort (i.e. number of recruited patients). If they don't require ICU admission within 15 days, they will stay in this cohort and be called "never ICU".
* Healthy volunteers: The investigators will recruit healthy volunteers among healthcare workers and allied personnel in the hospital. They will provide peripheral blood to serve as: a) independent controls for experimental and laboratory variables, and b) references of healthy baseline state for experiments.
* A patient previously enrolled as a COVID negative can be re-enrolled in a subsequent hospitalization with a new study ID (i.e. counts as another encounter for the total enrollment). The records will be linked to indicate they represent the same person enrolled again.

Exclusion Criteria

* Refusal to participate.
* Inability to record the primary outcome during the first 2 weeks.
* For the ICU positive and ICU negative cohorts only: failure to obtain the day 0 or 1 blood sample (for example technical problems, or identification of COVID-19 after ICU admission i.e. there was no suspicion on admission).
* For the ward patients, inability to collect day 1 or 2 blood sample.
* Known to have had COVID in the past (\>4 weeks) in any setting.
* Healthy volunteers only: COVID19 known or suspected infection, or unprotected exposure to a known acutely ill COVID19 patient in the past 4 weeks; and/or currently unwell or in the course of an acute illness.
Minimum Eligible Age

18 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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Dr. Andrew Baker

OTHER

Sponsor Role lead

Responsible Party

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Dr. Andrew Baker

Chief, Departments of Critical Care and Anesthesia, St Michael's Hospital

Responsibility Role SPONSOR_INVESTIGATOR

Principal Investigators

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Andrew J Baker, MD

Role: PRINCIPAL_INVESTIGATOR

Unity Health Toronto

Locations

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St Michael's Hospital

Toronto, Ontario, Canada

Site Status RECRUITING

Countries

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Canada

Central Contacts

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Marlene Santos

Role: CONTACT

416-360-4000 ext. 2322

Victoria Paz

Role: CONTACT

416-864-5559

Facility Contacts

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Marlene Santos

Role: primary

416-360-4000 ext. 2322

Victoria Paz

Role: backup

416-864-5559

References

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Trahtemberg U, Fritzler MJ; On behalf of the COVID-19 chapter of the "Longitudinal Biomarkers in Lung Injury" study group. COVID-19-associated autoimmunity as a feature of acute respiratory failure. Intensive Care Med. 2021 Jul;47(7):801-804. doi: 10.1007/s00134-021-06408-z. Epub 2021 Apr 30. No abstract available.

Reference Type DERIVED
PMID: 33928414 (View on PubMed)

Trahtemberg U, Rottapel R, Dos Santos CC, Slutsky AS, Baker A, Fritzler MJ. Anticardiolipin and other antiphospholipid antibodies in critically ill COVID-19 positive and negative patients. Ann Rheum Dis. 2021 Sep;80(9):1236-1240. doi: 10.1136/annrheumdis-2021-220206. Epub 2021 Apr 26.

Reference Type DERIVED
PMID: 33903092 (View on PubMed)

Other Identifiers

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COLOBILI v4.2

Identifier Type: -

Identifier Source: org_study_id

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