Identification of Entamoeba Spp. in Assuit Governorate Hospitals.

NCT ID: NCT04466449

Last Updated: 2022-03-09

Basic Information

• Recruitment Status: UNKNOWN
• Total Enrollment: 138 participants
• Study Classification: OBSERVATIONAL
• Study Start Date: 2022-09-30
• Study Completion Date: 2022-10-31

Brief Summary

1. Determination the prevalence of Entamoeba histolytica infections among patients at Assiut University Hospitals presented with diarrhea/dysentery.

2. Evaluatation the role of different cultures in diagnosing Entamoeba .

3. Molecularly differentiation between Entamoeba species to monitor the true prevalence of E.histolytica .

Detailed Description

Entamoeba histolytica is a common pathogenic protozoan widely distributed throughout the world (10% of the world's population), with 50,000-100,000 deaths reported annually(1) .It is a health problem both in Egypt and worldwide (2) The disease is more prevalent in densely populated areas with poor hygiene requirements and lacking sanitary water supply, especially in rural areas(3). It causes many digestive complications including diarrhea , and fever(2). it transmits through bloodstream forming abscesses in the brain, eye, liver and other vital organs and tissues or even lead to death in some cases . Some individuals have no symptom of the disease but transmit the infection to others(4) It is well- established that E. histolytica leads to invasive disease in humans. Although both E. dispar and E. moshkovskii are still considered to be non pathogenic and free-living amoebas, respectively, some reports suggest their potential role in provoking disease in humans (5) Cysts of E.dispar , E.moshkovskii and E.hartmanni can be confused with E.histolytica. cysts in the parasitological examination(6) The molecular assays, is preferable as it detects E. histolytica , differentiates it from other species and monitors its true prevalence for better treatment and effective control (2)

Conditions

Entamoeba Spp. Identification

Study Design

• Observational Model Type: OTHER
• Study Time Perspective: CROSS_SECTIONAL

Interventions

stool sample

A stool sample will be collected from each patient for diagnosis of Entamoeba

Intervention Type: DIAGNOSTIC_TEST

Eligibility Criteria

Inclusion Criteria

• : Male and female patients of different age groups with diarrhea/dysentery attending the outpatient clinics at Assiut University Hospitals .

• Eligible Sex: ALL
• Accepts Healthy Volunteers: No

Sponsors

Assiut University

OTHER

Sponsor Role: lead

Responsible Party

Youstina Talaat Basaly

YTalaatBasaly

Responsibility Role: PRINCIPAL_INVESTIGATOR

Locations

Youstina Talaat

Asyut, , Egypt

Site Status: RECRUITING

Countries

Egypt

Central Contacts

Youstina Talaat

Role: CONTACT

youstinatalaat63@gmail.com

01225530091

Abeer Lsayed Mahmoud

Role: CONTACT

abeerwns@yahoo.com

01028241777

Facility Contacts

youstina Talaat, demonestrator

Role: primary

youstinatalaat@yahoo.com

01225530091

Other Identifiers

Entamoeba spp. Identification

Identifier Type: -

Identifier Source: org_study_id