Antibacterial Effect of the Infusion of Green Tea Used as a Mouthwash on Saliva and Bacterial Plaque

NCT ID: NCT04410666

Last Updated: 2021-08-20

Basic Information

• Recruitment Status: COMPLETED
• Clinical Phase: NA
• Total Enrollment: 28 participants
• Study Classification: INTERVENTIONAL
• Study Start Date: 2016-08-21
• Study Completion Date: 2016-09-21

Brief Summary

Given that the participation of oral microorganisms in the development of the most prevalent pathologies of the oral cavity is unquestionable, efforts have been made to create agents that allow controlling these bacteria. The objective of this work was to analyze the antibacterial effect of green tea infusion used as a mouthwash on saliva and bacterial plaque. The green tea infusion was prepared at 13% at approximately 90 ° C, being the same applied to 14 participants from the 1st to the 5th grade (experimental group); and to other 14 participants the placebo was applied (control group). The antibacterial effect was determined by counting the colony forming units (CFU) in the cultures of samples of bacterial plaque and saliva, taken before the application of the infusion and immediately after.

Detailed Description

Oral diseases, including dental caries, is one of the most prevalent in the population. It is multifactorial and originates from the formation of the bacterial plaque and its non-elimination by physical and chemical hygiene methods.

The use of chemical agents to aid in the control of Bacterial Plaque has been the subject of study in many investigations. Today there is a variety of elements that can be use to carry out proper oral hygiene, such as toothbrushes, toothpastes, dental floss and mouthwash, all of them in a wide variety.

Often the lack of accessibility to these means problems in the hygiene in the population, either due to the lack of knowledge or the cost of obtaining them.

Therefore, the objective of analyzing the antibacterial effect of the infusion of green tea (camellia sinensis) used as a mouthwash on saliva and bacterial plaque was presented. The proposed research topic is relevant, since if green tea (camellia sinensis) is found to produce positive effects on bacterial plaque, it would serve to prevent its formation, thus helping to improve dental hygiene with an easy acquisition product and lower cost compared to other mouthwashes offered in the market, constituting a natural mouthwash.

Conditions

Dental Plaque

Study Design

• Allocation Method: RANDOMIZED
• Intervention Model: PARALLEL
• Primary Study Purpose: PREVENTION
• Blinding Strategy: SINGLE

Study Groups

GREEN TEA MOUTH WASH

an infusion at 13%, with 13 g of green tea (commercially divided) in 100 ml of saline solution, at a temperature of approximately 90 ° C.

Group Type: EXPERIMENTAL

Green Tea mouthwash

Intervention Type: OTHER

an infusion at 13%, with 13 g of green tea (commercially divided) in 100 ml of saline solution, at a temperature of approximately 90 ° C, in sterile glass containers.

Each application was 10 ml. applied for 1 minute.

Placebo

distilled water, in sterile glass containers.

Group Type: PLACEBO_COMPARATOR

Placebo Mouthwash

Intervention Type: OTHER

distilled water

Interventions

Green Tea mouthwash

an infusion at 13%, with 13 g of green tea (commercially divided) in 100 ml of saline solution, at a temperature of approximately 90 ° C, in sterile glass containers.

Each application was 10 ml. applied for 1 minute.

Intervention Type: OTHER

Placebo Mouthwash

distilled water

Intervention Type: OTHER

Eligibility Criteria

Inclusion Criteria

• Participants that signed the informed consent

Exclusion Criteria

• allergic to green tea (Camellia sinensis)
• ongoing medical or dental treatment that could interfere with the results.

• Minimum Eligible Age: 18 Years
• Eligible Sex: ALL
• Accepts Healthy Volunteers: Yes

Sponsors

Julieta María Méndez Romero

OTHER

Sponsor Role: lead

Responsible Party

Julieta María Méndez Romero

Head of Writting and Publication Department, Regional Health Research Institute

Responsibility Role: SPONSOR_INVESTIGATOR

Principal Investigators

Jazmin Servin

Role: PRINCIPAL_INVESTIGATOR

Facultad de Odontologia, Universidad Nacional de Caaguazu

Nelson Portillo

Role: STUDY_DIRECTOR

Facultad de Odontologia, Universidad Nacional de Caaguazu

Locations

Facultad de Odontología, Universidad nacional de Caaguazu

Coronel Oviedo, Caaguazú Department, Paraguay

Countries

Paraguay

References

Ardakani MR, Golmohammadi S, Ayremlou S, Taheri S, Daneshvar S, Meimandi M. Antibacterial effect of Iranian green-tea-containing mouthrinse vs chlorhexidine 0.2%: an in vitro study. Oral Health Prev Dent. 2014;12(2):157-62. doi: 10.3290/j.ohpd.a31663.

Reference Type: BACKGROUND

PMID: 24624389 (View on PubMed)

Megalaa N, Thirumurugan K, Kayalvizhi G, Sajeev R, Kayalvizhi EB, Ramesh V, Vargeese A. A comparative evaluation of the anticaries efficacy of herbal extracts (Tulsi and Black myrobalans) and sodium fluoride as mouthrinses in children: A randomized controlled trial. Indian J Dent Res. 2018 Nov-Dec;29(6):760-767. doi: 10.4103/ijdr.IJDR_790_16.

Reference Type: BACKGROUND

PMID: 30589005 (View on PubMed)

Ronanki S, Kulkarni S, Hemalatha R, Kumar M, Reddy P. Efficacy of commercially available chlorhexidine mouthrinses against specific oral microflora. Indian J Dent Res. 2016 Jan-Feb;27(1):48-53. doi: 10.4103/0970-9290.179816.

Reference Type: BACKGROUND

PMID: 27054861 (View on PubMed)

Hajiahmadi M, Yegdaneh A, Homayoni A, Parishani H, Moshkelgosha H, Salari-Moghaddam R. Comparative Evaluation of Efficacy of "Green Tea" and "Green Tea with Xylitol" Mouthwashes on the Salivary Streptococcus mutans and Lactobacillus Colony Count in Children: A Randomized Clinical Trial. J Contemp Dent Pract. 2019 Oct 1;20(10):1190-1194.

Reference Type: BACKGROUND

PMID: 31883255 (View on PubMed)

Servin J, Mendez J, Portillo N, Villasanti U. Antibacterial effect of green tea infusion used as a mouthwash on saliva and bacterial plaque: a randomized controlled trial. Gen Dent. 2021 Sep-Oct;69(5):72-74.

Reference Type: DERIVED

PMID: 34424217 (View on PubMed)

Other Identifiers

00139

Identifier Type: -

Identifier Source: org_study_id