Diagnostic Evaluation of Urine DNA Methylation/Somatic Mutation Profiling for the Detection of Urothelial Carcinoma

NCT ID: NCT04314245

Last Updated: 2023-05-24

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Total Enrollment

1170 participants

Study Classification

OBSERVATIONAL

Study Start Date

2019-08-01

Study Completion Date

2022-08-31

Brief Summary

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Clinical trial to evaluate the performance characteristics(sensitivity and specificity) of AnchorDx's urine DNA methylation/somatic mutation profiling assay for detecting urothelial carcinoma compared to pathology in patients.

Detailed Description

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This is a prospective study that involves 11 centers in China and 1200 participants. The assay analyzes the DNA methylation/somatic mutation profiles of bladder cancer-specific biomarkers non-invasively using urine specimens collected before invasive diagnosis/treatment. The performance characteristics(sensitivity and specificity) of the assay for detection of urothelial carcinoma is evaluated in comparison to pathology or cystoscopy.

Conditions

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Urothelial Carcinoma Bladder Cancer

Study Design

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Observational Model Type

COHORT

Study Time Perspective

PROSPECTIVE

Study Groups

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Urothelial carcinoma group

Subjects who diagnosed with incident or recurrent urothelial carcinoma (including bladder/ureter/renal pelvis) by surgical pathology.

Active Comparator: Pathology

Intervention Type OTHER

Routine treatment for bladder cancer, and surgical pathology for confirmation of patients with urothelial carcinoma.

Genetic test: AnchorDx's urine DNA methylation/somatic mutation profiling assay

Intervention Type OTHER

A urine-based assay for the detection of urothelial carcinoma using urine DNA methylation/somatic mutation profiling analysis.

interference group

Subjects who diagnosed with incident or recurrent bladder cancer other than urothelial carcinoma (including bladder squamous cell carcinoma/bladder adenocarcinoma/other bladder-related cancers/prostate cancer/rectal cancer) by surgical pathology.

Active Comparator: Pathology

Intervention Type OTHER

Routine treatment for bladder cancer, and surgical pathology for confirmation of patients with urothelial carcinoma.

Genetic test: AnchorDx's urine DNA methylation/somatic mutation profiling assay

Intervention Type OTHER

A urine-based assay for the detection of urothelial carcinoma using urine DNA methylation/somatic mutation profiling analysis.

Control group

Subjects who clinically diagnosed with benign disease of the urinary system, such as Urinary calculi, urinary tract infection (except urinary tuberculosis), benign prostatic hyperplasia, glandular cystitis.

Active Comparator: Clinical Diagnosis

Intervention Type OTHER

Routine clinical diagnosis and treatment of benign disease of the urinary system

Genetic test: AnchorDx's urine DNA methylation/somatic mutation profiling assay

Intervention Type OTHER

A urine-based assay for the detection of urothelial carcinoma using urine DNA methylation/somatic mutation profiling analysis.

Healthy volunteers group

Volunteers who have a normal routine urine test / ultrasound examination of the urinary system and do not carry suspected tumors of other organs.

Active Comparator: Clinical diagnosis

Intervention Type OTHER

Routine urine test and imaging diagnostic techniques, such as B-ultrasound.

Genetic test: AnchorDx's urine DNA methylation/somatic mutation profiling assay

Intervention Type OTHER

A urine-based assay for the detection of urothelial carcinoma using urine DNA methylation/somatic mutation profiling analysis.

Interventions

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Active Comparator: Pathology

Routine treatment for bladder cancer, and surgical pathology for confirmation of patients with urothelial carcinoma.

Intervention Type OTHER

Active Comparator: Clinical Diagnosis

Routine clinical diagnosis and treatment of benign disease of the urinary system

Intervention Type OTHER

Active Comparator: Clinical diagnosis

Routine urine test and imaging diagnostic techniques, such as B-ultrasound.

Intervention Type OTHER

Genetic test: AnchorDx's urine DNA methylation/somatic mutation profiling assay

A urine-based assay for the detection of urothelial carcinoma using urine DNA methylation/somatic mutation profiling analysis.

Intervention Type OTHER

Eligibility Criteria

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Inclusion Criteria

1. Urothelial carcinoma group:

* Any male or female patient aged 18 or older.
* Able to provide urine specimen (100ml for both first void and non-first-void urine) before treatments.
* Diagnosed with incident or recurrent urothelial carcinoma (including bladder/ureter/renal pelvis) by surgery.
2. interference group:

* Any male or female patient aged 18 or older.
* Able to provide urine specimen (100ml for both first void and non-first-void urine) before treatments.
* Diagnosed with incident or recurrent bladder cancer other than urothelial carcinoma (including bladder squamous cell carcinoma/bladder adenocarcinoma/other bladder-related cancers/prostate cancer/rectal cancer) by surgery.
3. Control group:

* Any male or female patient aged 18 or older.
* Able to provide urine specimen (100ml for both first void and non-first-void urine) before treatments.
* Diagnosed with urinary disease such as Urinary calculi, urinary tract infection (except urinary tuberculosis), benign prostatic hyperplasia, glandular cystitis. All enrollee are able to provide legally effective informed consent.
4. Healthy volunteers group:

* Any male or female patient aged 18 or older.
* Able to provide urine specimen (100ml for both first void and non-first-void urine) before physical examination.

Exclusion Criteria

1. Urothelial carcinoma/Interference group/Control group

* Has had diagnosed with other cancers.
* Patients diagnosed with non-urological cancer.
* Failed to provide a written informed consent.
2. Healthy volunteers group:

* Volunteers with abnormal test results of urine analysis or urological ultrasound test.
* Volunteers sceptical of cancers from non-urological origin in a normal results of urine analysis or urological ultrasound.
* Failed to provide legally effective informed consent.
Minimum Eligible Age

18 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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Sun Yat-Sen Memorial Hospital of Sun Yat-Sen University

OTHER

Sponsor Role collaborator

West China Hospital

OTHER

Sponsor Role collaborator

RenJi Hospital

OTHER

Sponsor Role collaborator

Tongji Hospital

OTHER

Sponsor Role collaborator

Peking University People's Hospital

OTHER

Sponsor Role collaborator

First Affiliated Hospital Xi'an Jiaotong University

OTHER

Sponsor Role collaborator

The First Affiliated Hospital with Nanjing Medical University

OTHER

Sponsor Role collaborator

Southern Medical University, China

OTHER

Sponsor Role collaborator

Nanfang Hospital, Southern Medical University

OTHER

Sponsor Role collaborator

Sichuan Cancer Hospital and Research Institute

OTHER

Sponsor Role collaborator

Affiliated Hospital & Clinical Medical College of Chengdu University

UNKNOWN

Sponsor Role collaborator

AnchorDx Medical Co., Ltd.

INDUSTRY

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Jian HUANG, MD

Role: STUDY_CHAIR

Sun Yat-Sen Memorial Hospital of Sun Yat-Sen University

Locations

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Sun Yat-sen Memorial Hospital, Sun Yat-sen University,

Guangzhou, Guangdong, China

Site Status

Countries

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China

Other Identifiers

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AnchorDx-BC004

Identifier Type: -

Identifier Source: org_study_id

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