Antibacterial Activity of Urea Against Ocular Bacteria

NCT ID: NCT04020406

Last Updated: 2019-07-16

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Total Enrollment

90 participants

Study Classification

OBSERVATIONAL

Study Start Date

2017-11-01

Study Completion Date

2018-11-01

Brief Summary

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Antibacterial properties of urea was tested against 35 isolates from ocular infections.

Detailed Description

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In vitro susceptibility testing was performed with commercially available antibiotic discs, homemade urea discs saturated with urea solution at a concentration of 96 mmol. The weight of urea in one drop = 0.37 mg, in one ml = 10.34 mg (5 microliter/ disc) discs and combined discs (antibiotic discs to which urea solution was added). Combined discs provided better in vitro susceptibility results than antibiotics alone.

Scanning electron micrographs of bacterial isolates incubated overnight with urea solution showed marked disruption of bacterial aggregative patterns and irregularities of their cell walls.

Western blotting showed marked reduction in total proteins in bacterial isolates incubated overnight with urea solution. These findings may explain the possible mechanisms for antibacterial activity of urea solution as a result of protein denaturation.

Conditions

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Urea in Blood; High

Study Design

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Observational Model Type

COHORT

Study Time Perspective

PROSPECTIVE

Study Groups

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Antibacterial Activity of Urea

Activity of Urea Solution against Ocular Bacterial Isolates

Antibacterial Activity

Intervention Type BIOLOGICAL

Antibacterial Activity of Urea Solution against Ocular Bacterial Isolates

Interventions

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Antibacterial Activity

Antibacterial Activity of Urea Solution against Ocular Bacterial Isolates

Intervention Type BIOLOGICAL

Eligibility Criteria

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Inclusion Criteria

* ocular infection

Exclusion Criteria

* Local antibiotics treatment
Minimum Eligible Age

5 Months

Maximum Eligible Age

65 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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Assiut University

OTHER

Sponsor Role lead

Responsible Party

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Hassan Lotfy Fahmy

Clinical Professor

Responsibility Role PRINCIPAL_INVESTIGATOR

Principal Investigators

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Hassan 58 Lotfy, Professor

Role: PRINCIPAL_INVESTIGATOR

Assiut T

Locations

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Hassan Lotfy Fahmy

Asyut, , Egypt

Site Status

Countries

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Egypt

Other Identifiers

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2323297

Identifier Type: -

Identifier Source: org_study_id

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