Use of Metabolomics for the Identification of Endometrial Biomarkers for IRPL and RIF After in Vitro Fertilization

NCT ID: NCT03690830

Last Updated: 2018-10-01

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

UNKNOWN

Clinical Phase

NA

Total Enrollment

40 participants

Study Classification

INTERVENTIONAL

Study Start Date

2019-01-01

Study Completion Date

2021-01-01

Brief Summary

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It is a case-control study composed of 3 groups : 2 cases groups (RIF and IRPL) already composed as part of a pre-existing research project and 1 control group including patients undergoing ART for male factor infertility.

The purpose of this study is to compare the 2 case group with the control group to identify metabolomics signatures.

Detailed Description

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The investigators have designed a unicentric, case-control, retro- and prospective study to compare the endometrial metabolomics profile in three groups of patients. The two case groups (RIF and IRPL) are already composed and the endometrial and blood samples already taken as part of a pre-existing research project. The current protocol concerns the control group that includes patients undergoing ART for male factor infertility.

The study will include three groups. The first group is composed of patients with a history of RIF following IVF-ET. RIF is defined as failure to obtain a clinical pregnancy (fetal cardiac activity at 6-7 weeks gestational age (GA)) following at least 3 different transfers of at least 4 good quality embryos (fresh or frozen).

The second group comprises patients with a history of IRPL, defined as three or more consecutive pregnancy losses that occurred before 14 weeks gestational age. Early pregnancy losses diagnosed before ultrasonographic confirmation will be included.

The third group will include patients undergoing ART for male factor infertility.

The study protocol will be presented and explained to all eligible patients in the control group during the routine consultation before treatment. Patients who agree to participate will contact the department to program the inclusion visit with one of the attending physicians in the department, during which the blood and endometrial samples will be taken. The inclusion visit will take place at the department of reproductive medicine of Angers University Hospital around day 21 of the menstrual cycle (in the middle of the implantation window, which occurs between day 19 and 23 of the cycle, day 1 being the first menstruation day).

The metabolomics analysis will be performed according to the same protocol for all samples (study and control groups)

Conditions

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Recurrent Pregnancy Loss Recurrent Implantation Failure

Study Design

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Allocation Method

NON_RANDOMIZED

Intervention Model

PARALLEL

Two case groups (Recurrent Implantation Failure and idiopathic recurrent pregnancy loss ) One control group (patients undergoing Assisted ReproductiveTechnologies (ART) for male factor infertility)
Primary Study Purpose

OTHER

Blinding Strategy

NONE

Study Groups

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Case group RIF

blood samples, analyzing endometrial cells

Group Type EXPERIMENTAL

metabolomics analysis

Intervention Type OTHER

collect blood samples and endometrial cells

Case group IRPL

blood samples, analyzing endometrial cells

Group Type EXPERIMENTAL

metabolomics analysis

Intervention Type OTHER

collect blood samples and endometrial cells

Control group

blood samples, analyzing endometrial cells

Group Type ACTIVE_COMPARATOR

metabolomics analysis

Intervention Type OTHER

collect blood samples and endometrial cells

Interventions

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metabolomics analysis

collect blood samples and endometrial cells

Intervention Type OTHER

Eligibility Criteria

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Inclusion Criteria

* Age between 18 (included) and 40 years (excluded).
* Patients who agreed to participate and signed the consent form
* Patients undergoing ART for male factor infertility: male partner with severe oligoasthenospermia or azoospermia.
* Patients without any prior history of infertility or risk factors for infertility other than the male factor.
* Patients with a normal basic infertility workup prior to ART (Day 3 serum FSH, LH, Estradiol and AMH; Antral follicle count; hysterosonography or hysterosalpingography)

Exclusion Criteria

* Non-French speaking patients.
* Major patients that are wards of the nation.

* Patients deprived of their liberty on criminal charges or for civil reasons.
* Patients under psychiatric care.
* Patients under legal protection.
* Patients unable to sign consent forms.
* Patients not covered by social security (which covers all treatments administered).
* Patients currently participating in other interventional research projects.
Minimum Eligible Age

18 Years

Maximum Eligible Age

39 Years

Eligible Sex

FEMALE

Accepts Healthy Volunteers

No

Sponsors

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University Hospital, Angers

OTHER_GOV

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Locations

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Angers University hospital

Angers, Maine Et Loire, France

Site Status

Countries

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France

Central Contacts

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Pierre-Emmanuel BOUET, MD

Role: CONTACT

+33(0)241354213

Other Identifiers

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RCB n°2018-A01871-54

Identifier Type: -

Identifier Source: org_study_id

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