Endothelial Cell Gene Networks of CVD

NCT ID: NCT03529916

Last Updated: 2021-07-28

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

WITHDRAWN

Study Classification

OBSERVATIONAL

Study Start Date

2018-05-21

Study Completion Date

2021-09-30

Brief Summary

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The objective of this study is to identify relevant signature gene networks of cardiovascular disease in endothelial cells derived from circulating endothelial progenitor cells of individuals with established cardiovascular disease (CVD).

Detailed Description

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The investigators propose to enroll CVD and healthy subjects between 30 and 60 years of age, matched for age, gender, body mass index, and medication (statin and anti-hypertensive) use. Circulating endothelial progenitor cells will be isolated from blood and cultured in vitro to differentiate into endothelial cells.

Conditions

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Coronary Heart Disease

Study Design

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Observational Model Type

CASE_CONTROL

Study Time Perspective

CROSS_SECTIONAL

Study Groups

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CVD subjects

CVD will be defined as \>50% stenosis of one or more coronary arteries as assessed by coronary angiography.

isolation of endothelial cells precursors in blood

Intervention Type OTHER

Circulating endothelial precursor cells will be isolated and then cultured in vitro. RNA will be then isolated.

healthy controls

healthy controls defined as not having stenosis of coronary arteries as assessed by coronary angiography.

isolation of endothelial cells precursors in blood

Intervention Type OTHER

Circulating endothelial precursor cells will be isolated and then cultured in vitro. RNA will be then isolated.

Interventions

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isolation of endothelial cells precursors in blood

Circulating endothelial precursor cells will be isolated and then cultured in vitro. RNA will be then isolated.

Intervention Type OTHER

Eligibility Criteria

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Inclusion Criteria

• \>50% stenosis of one or more coronary arteries


• absence of coronary artery stenosis

Exclusion Criteria

* type 2 diabetes mellitus
* chronic kidney disease,
* other chronic diseases
* dyslipidemia (fasting plasma LDL-C \>160 mg/dL and triglyceride levels \>150 mg/dL)
Minimum Eligible Age

30 Years

Maximum Eligible Age

60 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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Massachusetts General Hospital

OTHER

Sponsor Role collaborator

Tufts University

OTHER

Sponsor Role lead

Responsible Party

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Stefania lamon-Fava

Scientist I, Associate Professor

Responsibility Role PRINCIPAL_INVESTIGATOR

Principal Investigators

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Stefania Lamon-Fava, PhD

Role: PRINCIPAL_INVESTIGATOR

Tufts University

Locations

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Tufts Medical Center - Cardiovascular Clinic

Boston, Massachusetts, United States

Site Status

Countries

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United States

Other Identifiers

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12841

Identifier Type: -

Identifier Source: org_study_id

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