Effects of PA-1 Transcriptional Regulation on Monocyte Function

NCT ID: NCT00512369

Last Updated: 2011-07-18

Basic Information

• Recruitment Status: TERMINATED
• Total Enrollment: 125 participants
• Study Classification: OBSERVATIONAL
• Study Start Date: 2006-03-31
• Study Completion Date: 2011-07-31

Brief Summary

Researching genetic differences in people with no prior medical conditions for better understanding of cardiac diseases through genetic research.

Detailed Description

The discovery and subsequent application of small interfering RNA (siRNA) methods to achieve individual gene silencing in mammalian cells is a robust method that is well-described and validated in mammalian cell culture systems. We will apply this technique to achieve post-transcriptional "silencing" of PAI-1 in monocytes obtained from otherwise healthy volunteers, and study the subsequent loss of this gene's function on the migratory capacity of these cells in the proposed in vitro experimental system. This concept has not yet been demonstrated in the literature, and if validated, would be a novel and fundamental description of the role of PAI-1 in human monocyte biology as related to the development of atherosclerosis.

Conditions

Atherosclerosis

Study Design

• Study Time Perspective: PROSPECTIVE

Eligibility Criteria

Inclusion Criteria

• Ages 18 -50
• No current or past medical problems

Exclusion Criteria

• Patients taking prescription drugs (hormonal birth control or herbal supplements may be taken.

• Minimum Eligible Age: 18 Years
• Maximum Eligible Age: 50 Years
• Eligible Sex: ALL
• Accepts Healthy Volunteers: Yes

Sponsors

National Institutes of Health (NIH)

NIH

Sponsor Role: collaborator

Vanderbilt University

OTHER

Sponsor Role: lead

Responsible Party

Vanderbilt University

Principal Investigators

Mohan Sathyamoorthy, MD

Role: PRINCIPAL_INVESTIGATOR

Vanderbilt University

Locations

Vanderbilt University Medical Center

Nashville, Tennessee, United States

Countries

United States

Other Identifiers

NIH

Identifier Type: -

Identifier Source: secondary_id

060286

Identifier Type: -

Identifier Source: org_study_id