Comprehensive DNA Methylation Profiling in Crohn's Disease

NCT ID: NCT03272152

Last Updated: 2017-09-05

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

UNKNOWN

Total Enrollment

14 participants

Study Classification

OBSERVATIONAL

Study Start Date

2017-01-01

Study Completion Date

2017-09-30

Brief Summary

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Previous studies have indicated that abnormal DNA methylation frequently occurs in the mucosa in Crohn's disease. Comprehensive DNA methylation profiling of the inflamed and non-inflamed ileal mucosa of patients with Crohn's disease was performed. Genome-wide DNA methylation was determined using the Illumina HumanMethylation 850k BeadChip.

Detailed Description

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Conditions

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Crohn Disease Methylation Illumina Human Methylation 850k BeadChip

Study Design

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Observational Model Type

CASE_CONTROL

Study Time Perspective

PROSPECTIVE

Study Groups

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Inflamed CD group

Inflamed ileal mucosa was obtained from inflammed lesions of active CD patients

Genome-wide DNA methylation

Intervention Type GENETIC

Genome-wide DNA methylation was determined using the Infinium Human Methylation 850K BeadChip.

Non-inflamed CD group

Non-inflamed ileal mucosa was obtained from normal sites of active CD patients

Genome-wide DNA methylation

Intervention Type GENETIC

Genome-wide DNA methylation was determined using the Infinium Human Methylation 850K BeadChip.

Control group

Control ileal mucosa was obtained from healthy patients

Genome-wide DNA methylation

Intervention Type GENETIC

Genome-wide DNA methylation was determined using the Infinium Human Methylation 850K BeadChip.

Interventions

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Genome-wide DNA methylation

Genome-wide DNA methylation was determined using the Infinium Human Methylation 850K BeadChip.

Intervention Type GENETIC

Eligibility Criteria

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Inclusion Criteria

* Active Crohn's disease(CDAI score≄ 150)

Exclusion Criteria

* No ileal lesions
* Under treatment of immunomodulators, biologic agents or steroids
Minimum Eligible Age

18 Years

Maximum Eligible Age

60 Years

Eligible Sex

MALE

Accepts Healthy Volunteers

Yes

Sponsors

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Jinling Hospital, China

OTHER

Sponsor Role lead

Responsible Party

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Jianan Ren

Director of General Surgery Institute

Responsibility Role PRINCIPAL_INVESTIGATOR

Locations

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Jinling Hospital

Nanjing, Jiangsu, China

Site Status RECRUITING

Countries

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China

Facility Contacts

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Jianan Ren, MD, PhD

Role: primary

Yuan Li, MD

Role: backup

Other Identifiers

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20170808

Identifier Type: -

Identifier Source: org_study_id

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