Ascitic Fluid Microbiome in Liver Cirrhosis

NCT ID: NCT02813590

Last Updated: 2024-03-15

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

WITHDRAWN

Study Classification

OBSERVATIONAL

Study Start Date

2016-06-30

Study Completion Date

2017-09-30

Brief Summary

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Ascitic fluid microbiome is going to be investigated in this study by using internal transcripted spacer (ITS) and 16S ribosomal RNA (rRNA) polymerase chain reaction (PCR) and sequencing as conventional culture methods are lacking sensitivity regarding diagnosis of spontaneous bacterial peritonitis (SBP).

All patients with routinely performed ascitic fluid puncture and underlying liver cirrhosis are included in this study. Next generation sequencing (NGS) in ascitic fluid, skin swaps from puncture side and stool samples as well as conventional culture methods are performed to investigate the difference in the microbiome between patients with and without SBP.

Detailed Description

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Conditions

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Ascitic Fluid, Microbiota Microbiota Sequence Analysis Polymerase Chain Reaction

Study Design

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Observational Model Type

COHORT

Study Time Perspective

PROSPECTIVE

Study Groups

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Patients with liver cirrhosis and with SBP

Ascitic fluid puncture

Intervention Type OTHER

Skin swaps from puncture side

Intervention Type OTHER

Stool samples

Intervention Type OTHER

Patients with liver cirrhosis and without SBP

Ascitic fluid puncture

Intervention Type OTHER

Skin swaps from puncture side

Intervention Type OTHER

Stool samples

Intervention Type OTHER

Interventions

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Ascitic fluid puncture

Intervention Type OTHER

Skin swaps from puncture side

Intervention Type OTHER

Stool samples

Intervention Type OTHER

Eligibility Criteria

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Inclusion Criteria

* Underlying decompensated liver cirrhosis
* routinely performed ascitic fluid puncture
* informed consent

Exclusion Criteria

\-
Minimum Eligible Age

18 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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CBmed - Center for biomarker research in medicine

UNKNOWN

Sponsor Role collaborator

Medical University of Graz

OTHER

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Locations

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Medical University of Graz

Graz, Styria, Austria

Site Status

Countries

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Austria

Other Identifiers

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25-326 ex 12/13

Identifier Type: -

Identifier Source: org_study_id

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