Clinical Trial With Broccoli Sprout Extract to Patients With Type 2 Diabetes

NCT ID: NCT02801448

Last Updated: 2022-01-19

Basic Information

• Recruitment Status: COMPLETED
• Clinical Phase: PHASE2
• Total Enrollment: 103 participants
• Study Classification: INTERVENTIONAL
• Study Start Date: 2015-09-30
• Study Completion Date: 2020-06-30

Brief Summary

Type 2 diabetes (T2D) results from a combination of insufficient insulin secretion from pancreatic islets and insulin resistance of target cells. The investigators have extensive pre-clinical data suggesting that BSE through its high content of the isothiocyanate sulforaphane improves hepatic insulin sensitivity. BSE as a dietary supplement could therefore benefit both patients with T2D and individuals at risk for the disease. BSE-containing sulforaphane is suggested to activate Nuclear factor-like 2 (NRF2). The investigators aim to study the clinical effect of using BSE as a dietary supplement on glucose tolerance and insulin sensitivity.

Detailed Description

Sulforaphane binds to Keap1 in the cytosol, leading to nuclear translocation of this transcription factor. In the nucleus NRF2 induces the expression of a large number of anti-oxidative genes. Sulforaphane is contained at high concentration in broccoli sprout extracts (BSE). Human studies have been conducted using broccoli sprout products without complication. It is being tested for the treatment or prevention of cancer and inflammatory diseases in ~30 clinical trials without any serious adverse events reported. The low toxicity makes BSE ideal as a dietary supplement for preventing and treating T2D.

The investigators aim to study the clinical effect of sulforaphane on glucose tolerance in T2D patients. Sulforaphane will be given as BSE. BSE is a freeze-dried powder of an aqueous extract of broccoli sprouts that provides a consistent and stable source of sulforaphane. The investigators will use a parallel arm study design with patients receiving BSE or placebo. The randomization will be double-blind. The study will be done at one centre. Patients with BMI between 25-40 kg/m2 will be included. Another inclusion criterion is HbA1c 6-10%. Patients will be treated for 12 weeks to enable us to observe effects on HbA1c (HbA1c turn-over is 3 months).

Patients will come to a screening visit and if they give informed consent and are included they attend a second visit 2-3 weeks later.

The patients will undergo an oral glucose tolerance test (OGTT) before and after the 12-week treatment period. The reason for using OGTT rather than e.g. insulin clamps as the readout is that it is a harmless standard procedure that gives an integrated view of glucose tolerance.

Conditions

Diabetes Mellitus, Non-Insulin-Dependent

Study Design

• Allocation Method: RANDOMIZED
• Intervention Model: PARALLEL
• Primary Study Purpose: TREATMENT
• Blinding Strategy: QUADRUPLE

Study Groups

Placebo

Placebo containing maltodextrine but no active sulforaphane

Group Type: PLACEBO_COMPARATOR

Placebo

Intervention Type: DRUG

Maltodextrine-based placebo without sulforaphane

BSE

Broccoli sprout extract once daily for 12 weeks

Group Type: ACTIVE_COMPARATOR

sulforaphane

Intervention Type: DRUG

sulforaphane-containing broccoli sprout extracts

Interventions

sulforaphane

sulforaphane-containing broccoli sprout extracts

Intervention Type: DRUG

Placebo

Maltodextrine-based placebo without sulforaphane

Intervention Type: DRUG

Eligibility Criteria

Inclusion Criteria

• Classified as type 2 diabetes
• Written informed consent
• Age: for men and women 35-75 years; for women below 75 years should be postmenopausal (defined as no menstrual bleeding since at least one year).
• Body mass index 25-40 kg/m2
• At screening visit : HbA1c 6-10 %, equivalent to 41-86 mmol/mol
• Currently treated with metformin or diet

Exclusion Criteria

• Treatment with insulin, other anti-diabetic treatment given as injections or any oral anti-diabetic treatment except metformin
• Fasting blood glucose at screening > 15.0 mmol/L
• Active liver disease
• At screening or at any subsequent visit a level of aspartate aminotransferase (ASAT) or alanine aminotransferase (ALAT) of more than three times the upper limit of the normal range
• Gastrointestinal ailments which may interfere with the ability to adequately absorb sulforaphane
• At screening visit creatinine > 130 µmol/L
• Coagulation disorder or current anti-coagulant therapy with warfarin, which may be affected by the BSE
• Diagnosed with a cardiovascular disease or event within 6 months prior to enrolment
• Systemic glucocorticoid treatment
• Herbal treatment, defined as food supplement (except multivitamin treatment) with herbal or vegetable extracts that may contain sulforaphane
• Allergy to broccoli
• Mental disorder making the patient unable to understand the study information
• Participation in other clinical trial which may affect the outcome of the present study
• Any other physical or psychiatric condition or treatment that in the judgment of the investigator makes it difficult to participate in the study.

• Minimum Eligible Age: 35 Years
• Maximum Eligible Age: 75 Years
• Eligible Sex: ALL
• Accepts Healthy Volunteers: No

Sponsors

Anders Rosengren, MD PhD

OTHER

Sponsor Role: lead

Responsible Party

Anders Rosengren, MD PhD

MD PhD

Responsibility Role: SPONSOR_INVESTIGATOR

Principal Investigators

Anders Rosengren

Role: PRINCIPAL_INVESTIGATOR

Lund University

References

Axelsson AS, Tubbs E, Mecham B, Chacko S, Nenonen HA, Tang Y, Fahey JW, Derry JMJ, Wollheim CB, Wierup N, Haymond MW, Friend SH, Mulder H, Rosengren AH. Sulforaphane reduces hepatic glucose production and improves glucose control in patients with type 2 diabetes. Sci Transl Med. 2017 Jun 14;9(394):eaah4477. doi: 10.1126/scitranslmed.aah4477.

Reference Type: DERIVED

PMID: 28615356 (View on PubMed)

Other Identifiers

BSE1

Identifier Type: -

Identifier Source: org_study_id