Intestinal Microbiota Composition After Antibiotic Treatment in Early Life

NCT ID: NCT02536560

Last Updated: 2015-09-01

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

UNKNOWN

Total Enrollment

450 participants

Study Classification

OBSERVATIONAL

Study Start Date

2012-01-31

Study Completion Date

2016-10-31

Brief Summary

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In this prospective observational cohort study the potential clinical consequences of antibiotic use in early life and perturbations in the gastrointestinal microbiota composition due to that antibiotic use are studied. It is hypothesized that altered microbiota may be an important underlying mechanism for impediments in the developing immune system.

Differentiation will be made between a group of neonates who received antibiotics in the first week of life, and control infants who were not exposed to antibiotics in the neonatal period.

Detailed Description

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Healthy newborns born in the hospital, observed for low probability of neonatal infection will be compared to newborns exposed to antibiotic therapy in early life (first 1-2 weeks).

Infants are recruited from the maternity wards and neonatal wards of four teaching hospitals in the Netherlands. In total 150 infants, treated with antibiotics because of (a high suspicion of) a perinatal infection during the first week of life, will be recruited. The control group comprises 300 healthy newborns, born in the hospital and needing clinical observation for 24-48 hours for several reasons like maternal comorbidity, low probability of neonatal infection, blood sugar monitoring, meconium containing amniotic fluid, or delivery by caesarean section.

Differences in clinical outcomes between antibiotic treated infants and controls are investigated. Incidence of atopic dermatitis (eczema), food allergy, upper respiratory tract infections (URTI), lower respiratory tract infections (LRTI), gastrointestinal infections (GITI) and excessive crying are evaluated, prospectively assessed by parental reports and retrospectively assessed by doctor's diagnoses. The clinical endpoints will be linked to the developing intestinal microbiota during the first year of life.

Potential differences in intestinal fecal microbiota composition and diversity can be determined at eight time points during the first year of life, as sampling moments include: day one (T1), day two (T2), one week (T3), two weeks (T4), one month (T5), three months (T6), six months (T7), one year (T8).

Conditions

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Microbiota Eczema Atopy

Study Design

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Observational Model Type

COHORT

Study Time Perspective

PROSPECTIVE

Study Groups

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Antibiotics

150 infants, (because of hospital protocol) treated with antibiotics because of a perinatal infection during the first week of life

No interventions assigned to this group

Controls

The control group comprises 300 healthy newborns, born in the hospital and needing clinical observation for 24-48 hours for several reasons like maternal comorbidity, low probability of neonatal infection, blood sugar monitoring, meconium containing amniotic fluid, or delivery by caesarean section

No interventions assigned to this group

Eligibility Criteria

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Inclusion Criteria

1. Term-born babies (≥ 36 weeks gestational age)
2. (Short) stay on maternal ward or admission to neonatal ward because of antibiotic treatment
3. Signed informed consent by the parents

Exclusion Criteria

1. Congenital illness or malformations
2. Severe perinatal infections for which transfer to the neonatal intensive care unit is needed
3. Maternal probiotic use ≤ six weeks before delivery
4. Insufficient knowledge of the Dutch language.
Minimum Eligible Age

1 Hour

Maximum Eligible Age

7 Days

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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Danone Global Research & Innovation Center

INDUSTRY

Sponsor Role collaborator

Amsterdam UMC, location VUmc

OTHER

Sponsor Role collaborator

Agentschap NL

OTHER_GOV

Sponsor Role lead

Responsible Party

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Nicole B.M.M. Rutten

PhD candidate

Responsibility Role PRINCIPAL_INVESTIGATOR

Principal Investigators

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Arine M Vlieger, MD, PhD

Role: PRINCIPAL_INVESTIGATOR

St Antonius Hospital Nieuwegein, the Netherlands

Locations

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Gelre Hospitals

Apeldoorn, Gelderland, Netherlands

Site Status RECRUITING

Meander Medical Centre

Amersfoort, Utrecht, Netherlands

Site Status RECRUITING

Tergooi Hospital

Blaricum, Utrecht, Netherlands

Site Status RECRUITING

St Antonius Hospital

Nieuwegein, Utrecht, Netherlands

Site Status RECRUITING

Countries

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Netherlands

Central Contacts

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Nicole B Rutten, MD

Role: CONTACT

+31 88 320 6325

Arine M Vlieger, MD, PhD

Role: CONTACT

+31 88 320 6325

Facility Contacts

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Johanna H Oudshoorn, MD, PhD

Role: primary

+31 55 581 8181

Clemens B Meijssen, MD

Role: primary

+31 33 850 5050

Clarissa E Crijns, MD

Role: primary

088 753 1753

Arine M Vlieger, MD, PhD

Role: primary

+31 88 320 6325

Carin Bunkers

Role: backup

+31 88 320 6325

References

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Daele EV, Kamphorst K, Belzer C, van Elburg RM, Knol J, Smidt H, Vlieger AM. Aberrant microbiota signatures precede symptom development in infantile colic. J Pediatr Gastroenterol Nutr. 2025 Aug;81(2):217-225. doi: 10.1002/jpn3.70101. Epub 2025 Jun 9.

Reference Type DERIVED
PMID: 40485532 (View on PubMed)

An R, Fontana F, Van Daele E, Ventura M, Vlieger A, van Elburg RM, Knol J, Milani C, Belzer C. Longitudinal changes in bifidobacterial population during the first two years of life. Benef Microbes. 2024 Apr 30;15(3):227-240. doi: 10.1163/18762891-bja00012.

Reference Type DERIVED
PMID: 38677714 (View on PubMed)

Kamphorst K, Vlieger AM, Oosterloo BC, Garssen J, van Elburg RM. Neonatal Antibiotics and Food Allergy Are Associated With FGIDs at 4-6 Years of Age. J Pediatr Gastroenterol Nutr. 2022 Jun 1;74(6):770-775. doi: 10.1097/MPG.0000000000003428. Epub 2022 Feb 24.

Reference Type DERIVED
PMID: 35588166 (View on PubMed)

Oosterloo BC, Van't Land B, de Jager W, Rutten NB, Klopping M, Garssen J, Vlieger AM, van Elburg RM. Neonatal Antibiotic Treatment Is Associated With an Altered Circulating Immune Marker Profile at 1 Year of Age. Front Immunol. 2020 Jan 10;10:2939. doi: 10.3389/fimmu.2019.02939. eCollection 2019.

Reference Type DERIVED
PMID: 31998285 (View on PubMed)

Kamphorst K, Oosterloo BC, Vlieger AM, Rutten NB, Bunkers CM, Wit EC, van Elburg RM. Antibiotic Treatment in the First Week of Life Impacts the Growth Trajectory in the First Year of Life in Term Infants. J Pediatr Gastroenterol Nutr. 2019 Jul;69(1):131-136. doi: 10.1097/MPG.0000000000002360.

Reference Type DERIVED
PMID: 31058782 (View on PubMed)

Rutten NB, Rijkers GT, Meijssen CB, Crijns CE, Oudshoorn JH, van der Ent CK, Vlieger AM. Intestinal microbiota composition after antibiotic treatment in early life: the INCA study. BMC Pediatr. 2015 Dec 9;15:204. doi: 10.1186/s12887-015-0519-0.

Reference Type DERIVED
PMID: 26645894 (View on PubMed)

Other Identifiers

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NL.37233.100.11, R-11.26AM

Identifier Type: -

Identifier Source: org_study_id

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