EmbryoGen/ Blastgen for Couples With Implantation Problems or Previous Miscarriage

NCT ID: NCT02305420

Last Updated: 2018-02-19

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

PHASE4

Total Enrollment

100 participants

Study Classification

INTERVENTIONAL

Study Start Date

2014-11-30

Study Completion Date

2017-06-14

Brief Summary

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EmbryoGen and BlastGen contain the cytokine growth factor Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF), which has been documented to give significant benefit to this difficult group of patients.

Results showed a highly significant effect of 44% relative improvement in ongoing implantation rate (p=0.001) in women who have previously miscarried (Ziebe et al 2013).

We wish to undertake a randomised Controlled trial to determine if EmbryoGen/BlastGen media improves pregnancy outcomes in women with recurrent implantation failure, recurrent miscarriage and poor embryo development when compared to standard media.

Detailed Description

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A randomised control pilot study will be undertaken for 100 women who have implantation failure (≥ 2 embryo transfers without a pregnancy) or at least 1 previous miscarriage and are having a further IVF cycle.

At oocyte retrieval each couple will be randomised using numbered envelopes containing pre randomised group allocations by a person uninvolved in the trial to:

1. BlastGen Group (n=50): culturing their embryos in ORIGIO standard, sequential culture mediums for 3 days (EmbryoGen included) then BlastGen media (GM-CSF containing media) for remaining 2 days or
2. Standard Media Group (n=50): culturing their embryos in Cook standard sequential medium for 5 days.

The best single embryo will be transferred on day 5.

The clinical staff and participating couple will be blinded as to whether BlastGen or standard media was used in culture to day 5. Fertility SA embryologists will not be blind as to which study group a couple is in and which media is being used. The embryos will be cultured using the usual methods and lab facilities by Fertility SA's current embryology staff members who will not be blind to the media being used.

Records on the outcome of the oocyte fertilisation, blastocyst development, implantation rate and pregnancy rates will be recorded, as is standard practice. Pregnancy scans will be carried out at 7 and 12 weeks. Live birth outcomes and any congenital anomalies will be recorded.

Conditions

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Recurrent Miscarriage Infertility

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

CROSSOVER

Randomised control trial comparing EmbryoGen/ BlastGen media use with standard media in IVF
Primary Study Purpose

TREATMENT

Blinding Strategy

QUADRUPLE

Participants Caregivers Investigators Outcome Assessors

Study Groups

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EmbryoGen/BlastGen

EmbryoGen and BlasGen media containing GM-CSF 2ng/ml will be used in the experimental arm

The intervention is to use EmbryoGen/BlastGen

Group Type EXPERIMENTAL

EmbryoGen/ BlastGen media

Intervention Type DEVICE

Embryos will be cultured in media containing Gm-CSF (EmbryoGen/ BlastGen media)

Control

Standard Cook sequential media

Group Type ACTIVE_COMPARATOR

Standard Cook sequential media

Intervention Type DEVICE

Standard Cook IVF media will be used in the control arm

Interventions

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EmbryoGen/ BlastGen media

Embryos will be cultured in media containing Gm-CSF (EmbryoGen/ BlastGen media)

Intervention Type DEVICE

Standard Cook sequential media

Standard Cook IVF media will be used in the control arm

Intervention Type DEVICE

Other Intervention Names

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GM-CSF containing media

Eligibility Criteria

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Inclusion Criteria

* women undertaking an IVF or ICSI cycle

* aged between 25 and 41 years
* 2 or more embryos transferred without a positive pregnancy outcome OR a history of at least 1 previous pregnancy loss, OR
* Poor embryo development (\<20% of embryo developing on time at Day 3 or no blastocyst above Grade 2 on Day 5)

Exclusion Criteria

* male partner requiring surgical retrieval of sperm except in cases of previous vasectomy
* the use of another investigational drug within 30 days
* any severe chronic disease that would significantly influence an IVF cycle
* less than 3 follicles\>14mm on ultrasound on the day of HCG trigger
Minimum Eligible Age

25 Years

Maximum Eligible Age

41 Years

Eligible Sex

FEMALE

Accepts Healthy Volunteers

No

Sponsors

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University of Adelaide

OTHER

Sponsor Role collaborator

FertilitySA

INDUSTRY

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Locations

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FertilitySA

Adelaide, South Australia, Australia

Site Status

Countries

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Australia

References

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Rose RD, Barry MF, Dunstan EV, Yuen SM, Cameron LP, Knight EJ, Norman RJ, Hull ML. The BlastGen study: a randomized controlled trial of blastocyst media supplemented with granulocyte-macrophage colony-stimulating factor. Reprod Biomed Online. 2020 May;40(5):645-652. doi: 10.1016/j.rbmo.2020.01.011. Epub 2020 Jan 23.

Reference Type DERIVED
PMID: 32220517 (View on PubMed)

Other Identifiers

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8114

Identifier Type: -

Identifier Source: org_study_id

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