Comparison of the Methylation Pattern of DNA in Buccal Swabs From Different Time Points
NCT ID: NCT02297009
Last Updated: 2024-07-16
Study Results
The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.
Basic Information
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TERMINATED
20 participants
OBSERVATIONAL
2014-12-31
2024-07-31
Brief Summary
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DNA will be extracted from the buccal cells on the swabs using a commercial extraction kit and will be quantified by a nano-drop spectrophotometer.
We will determine global and gene-specific methylation and hydroxymethylation alterations by UPLC/MS/MS. Hydrolyzed DNA into individual nucleosides will be analyzed for the quantitative measurement of 5-methylCytosine and 5-hydroxymethylCytosine on Triple Quadrupole UPLC/MS/MS platform. Secondly, specific methylation in the CpG islands of tumor suppressor and promoter genes, and genes involved in the oxidative stress pathways will be assessed by PCR-pyrosequencing.
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Detailed Description
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Conditions
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Study Design
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COHORT
PROSPECTIVE
Study Groups
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Volunteers
30 volunteers, half men and half women Buccal swab
Buccal swab
Buccal swabs will be taken from the volunteers
Interventions
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Buccal swab
Buccal swabs will be taken from the volunteers
Eligibility Criteria
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Inclusion Criteria
Exclusion Criteria
18 Years
35 Years
ALL
Yes
Sponsors
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KU Leuven
OTHER
Universitaire Ziekenhuizen KU Leuven
OTHER
Responsible Party
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Locations
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BIOMAT
Leuven, , Belgium
Countries
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Other Identifiers
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S57170
Identifier Type: -
Identifier Source: org_study_id
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