Biomarkers for Intestinal Permeability in Patients With Constipation

NCT ID: NCT02246647

Last Updated: 2019-08-21

Study Results

Results available

Outcome measurements, participant flow, baseline characteristics, and adverse events have been published for this study.

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Basic Information

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Recruitment Status

COMPLETED

Total Enrollment

39 participants

Study Classification

OBSERVATIONAL

Study Start Date

2014-09-30

Study Completion Date

2016-12-08

Brief Summary

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Our overall objective with this study is firstly to provide a comprehensive assessment of intestinal permeability, mucosal barrier function using existing biomarkers and secondly to explore novel biomarkers for measuring intestinal permeability in patients with constipation predominant Irritable Bowel Syndrome (IBS-C).

Detailed Description

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In order to determine the differences in permeability in IBS-C in comparison with healthy volunteers, the following will be determined: differences in in vivo small intestinal and colonic permeability, differences in small intestinal and colonic mucosal barrier function, differences in effects of fecal supernatants on barrier function of T84 monolayers, and differences in novel biomarkers for intestinal permeability

Conditions

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Intestinal Diseases Irritable Bowel Syndrome Constipation

Study Design

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Observational Model Type

CASE_CONTROL

Study Time Perspective

CROSS_SECTIONAL

Study Groups

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Healthy volunteers

Permeability measurement: Ingestion of saccharides {mannitol (regular, 12C) 100 mg, lactulose 1 g and labelled (13C mannitol) 100 mg} in 250ml of water Esophagogastroduodenoscopy Flexible sigmoidoscopy

Permeability measurement

Intervention Type DIAGNOSTIC_TEST

Saccharide excretion was compared between IBS-C and healthy volunteers

Esophagogastroduodenoscopy

Intervention Type PROCEDURE

Duodenal biopsies were collected from IBS-C and healthy volunteers

Flexible sigmoidoscopy

Intervention Type PROCEDURE

Colonic biopsies were collected from IBS-C and healthy volunteers

IBS-C

Permeability measurement: Ingestion of saccharides (mannitol (regular, 12C) 100 mg, lactulose 1 g and labelled (13C mannitol) 100 mg} in 250ml of water Esophagogastroduodenoscopy Flexible sigmoidoscopy

Permeability measurement

Intervention Type DIAGNOSTIC_TEST

Saccharide excretion was compared between IBS-C and healthy volunteers

Esophagogastroduodenoscopy

Intervention Type PROCEDURE

Duodenal biopsies were collected from IBS-C and healthy volunteers

Flexible sigmoidoscopy

Intervention Type PROCEDURE

Colonic biopsies were collected from IBS-C and healthy volunteers

Interventions

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Permeability measurement

Saccharide excretion was compared between IBS-C and healthy volunteers

Intervention Type DIAGNOSTIC_TEST

Esophagogastroduodenoscopy

Duodenal biopsies were collected from IBS-C and healthy volunteers

Intervention Type PROCEDURE

Flexible sigmoidoscopy

Colonic biopsies were collected from IBS-C and healthy volunteers

Intervention Type PROCEDURE

Other Intervention Names

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12C Mannitol 13C Mannitol Lactulose

Eligibility Criteria

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Inclusion Criteria

1. 18 - 65 years old
2. IBS-C by Rome III criteria (for IBS-C participants)
3. No abdominal surgery (except appendectomy and cholecystectomy)

Exclusion Criteria

1. History of Inflammatory Bowel Disease (IBD) , microscopic colitis or celiac disease
2. Use of tobacco products within the past 6 months
3. Use of NSAIDs or aspirin within the past week
4. Use of oral corticosteroids within the previous 6 weeks
5. Ingestion of artificial sweeteners such as Splenda (sucralose), Nutrasweet (aspartame), lactulose or mannitol 2 days before the study begins, e.g., foods to be avoided are sugarless gums or mints and diet soda
6. Ingestion of any prescription, over the counter, or herbal medications which can affect gastrointestinal transit 7 days before study begins

1. Any treatment specifically taken for IBS, including loperamide, cholestyramine, alosetron
2. Drugs with a known pharmacological activity at 5-HT4, 5-HT2b or 5-HT3 receptors (e.g, tegaserod, ondansetron, tropisetron, granisetron, dolasetron, mirtazapine);
3. All narcotics (e.g, codeine, morphine, and propoxyphene, either alone or in combination)
4. Anti-cholinergic agents (e.g, dicyclomine, hyoscyamine, propantheline).
5. Ultram
6. GI preparations

* Anti-nausea agents (e.g, trimethobenzamide, promethazine, prochlorperazine, dimenhydrinate, hydroxyzine)
* Osmotic laxative agents (e.g, lactulose, sorbitol or PEG solutions as Miralax and Glycolax)
* Prokinetic agents (e.g, cisapride, metoclopramide, itopride, domperidone);
7. Antimuscarinics;
8. Peppermint oil;
9. Systemic antibiotics, rifaximin, metronidazole.
7. Bleeding disorders or medications that increase risk of bleeding from mucosal biopsies.
8. Score \> 8 for anxiety or depression on Hospital anxiety and depression scale.
9. Pregnancy
Minimum Eligible Age

18 Years

Maximum Eligible Age

65 Years

Eligible Sex

FEMALE

Accepts Healthy Volunteers

Yes

Sponsors

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Takeda Pharmaceuticals International, Inc.

INDUSTRY

Sponsor Role collaborator

Mayo Clinic

OTHER

Sponsor Role lead

Responsible Party

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Madhusudan (Madhu) Grover, MBBS

Principal Investigator

Responsibility Role PRINCIPAL_INVESTIGATOR

Principal Investigators

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Madhusudan Grover, MBBS

Role: PRINCIPAL_INVESTIGATOR

Mayo Clinic

Locations

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Mayo Clinic in Rochester

Rochester, Minnesota, United States

Site Status

Countries

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United States

Other Identifiers

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14-002382

Identifier Type: -

Identifier Source: org_study_id

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