Targeted Chemo-elimination (TCE) of Malaria

NCT ID: NCT01872702

Last Updated: 2020-08-28

Basic Information

• Recruitment Status: COMPLETED
• Clinical Phase: NA
• Total Enrollment: 8000 participants
• Study Classification: INTERVENTIONAL
• Study Start Date: 2013-04-30
• Study Completion Date: 2017-07-31

Brief Summary

The overall aim of this study is two fold:

1. to pilot targeted chemo-elimination of plasmodium falciparum malaria in known areas of artemisinin resistance in South East Asia.

2. to understand the micro-epidemiology of malaria in these areas; chiefly, the prevalence and importance to on-going transmission of sub-clinical p.f malaria infections.

Detailed Description

The spread of artemisinin resistance in Plasmodium falciparum, which compromises the therapeutic efficacy of artemisinin combination treatments (ACTs), is the greatest threat to current global initiatives to control and eliminate malaria and is considered the highest priority of the WHO Global Malaria Programme. If not eliminated, resistant parasites could spread across Asia to Africa, as happened with resistance to other antimalarials in the past.

Conventional descriptions of the epidemiology of malaria in low transmission settings suggest that malaria prevalences are low (<10%) and heterogeneous. Most or all infections are thought to be symptomatic so the focus of malaria control activities is on the identification and treatment of symptomatic individuals. We and others have shown recently that artemisinin resistant P. falciparum is prevalent in Western Cambodia, and that it is now also found along the Thailand-Myanmar border and Vietnam. We have recently developed highly sensitive quantitative PCR (uPCR) methods for parasite detection using >1mL of blood which are 5,000 times more sensitive than conventional microscopy, and 100 times more sensitive than currently used PCR.

We have studied villages along the Thai-Myanmar border which are typical for the region and are classified by conventional epidemiological techniques as low-transmission (5-20% malaria prevalence). Our studies suggest that the majority of the population is infected. In Pailin, Western Cambodia, in areas where the National Malaria Control Programme and WHO believe that malaria has been all but eliminated, we have also found very high rates (>80%) of sub-microscopic parasitaemia in patients with fever or history of fever who are RDT negative. Thus, there is a lot more asymptomatic malaria in low transmission settings than previously thought, suggesting that control and elimination activities need to be rethought.

Highly sensitive quantitative PCR (uPCR) requires a venous blood sample, a laboratory which can perform vacuum DNA extraction, and on average four weeks for processing. A rapid highly sensitive diagnostic test which can be performed at the point of care would be a technological breakthrough. Screening with highly sensitive RDTs and treating of asymptomatic carriers will have a range of public health applications. Such tests are becoming available in 2017 and will be evaluated side by side with uPCR.

This study is designed to conduct and evaluate the efficacy of pilot implementation of targeted chemo-elimination in selected areas with the goal of eliminating malaria in these regions. This differs from mass drug administration (MDA); it is a strategy used to identify specific areas where mass treatment is necessary, in this case to eliminate all malaria parasites. Elimination will be targeted at communities with significant levels of subclinical infection and transmission which will be identifiable in the future by comparing rates of positivity by RDT or microscopy from new population samples against our qPCR data, which shows the true falciparum prevalence.

The study will assess the feasibility, safety and acceptability of this strategy and its impact on the transmission of malaria and the progression of artemisinin resistance. In addition it will evaluate the contribution of low parasitaemia carriage to transmission of artemisinin resistant malaria. These pilot studies are a necessary prelude to future scale up and policy implementation.

Dihydroartemisinin-piperaquine (DP) is a highly efficacious and inexpensive ACT which is well tolerated by all age groups when used to treat uncomplicated multi-drug resistant falciparum malaria in South East Asia. Monthly DP treatments have proved highly effective and well tolerated. When used as part of a MDA strategy, the addition of a gametocytocidal drug contributes towards the goal of malaria elimination by adding a strong transmission blocking activity to the regimen. Primaquine (PQ), the only currently licensed 8-aminoquinoline, is relatively safe and very effective when used at a dose of 0.25 mg base/kg, and does not require G6PD screening. Thus, we propose to evaluate the potential of this strategy to eliminatie malaria focally in areas where artemisinin resistance in P. falciparum is prevalent using DP plus PQ.

Conditions

Plasmodium Falciparum Malaria

Study Design

• Allocation Method: RANDOMIZED
• Intervention Model: PARALLEL
• Primary Study Purpose: TREATMENT
• Blinding Strategy: NONE

Study Groups

malaria elimination using DP and low-dose primaquine

Two villages randomly allocated to intervention (chemo-elimination) at each of the 4 sites (population approximately 500 people in each village). In these villages the entire population will be invited to receive three, monthly rounds of treatment with dihydroartemisinin-piperaquine and primaqunine to kill malaria parasites. The micro-epidemiology of malaria will be studied and prevalence and patterns of transmission used for comparison. NB, in Cambodia there will be no intervention villages and all four villages will be used to study the micro-epidemiology of malaria transmission in the absence of malaria elimination.

Group Type: EXPERIMENTAL

malaria elimination using DP and low-dose primaquine

Intervention Type: DRUG

Treatment of all persons resident in the intervention villages including those who do not have malaria parasites as detected by rapid diagnostic test. This is to interrupt p.f malaria transmission by removing the reservoir of all potentially infectious people from the area.

Control villages

Two villages randomly allocated to control (no chemo-elimination) at each of the 4 sites (population approximately 500 people in each village). In these villages only the micro-epidemiology of malaria will be studied and prevalence and patterns of transmission used for comparison. NB, in Cambodia there will be no intervention villages and all four villages will be used to study the micro-epidemiology of malaria transmission in the absence of malaria elimination.

From June 2013 to June 2014 Cambodia site conducted surveys with no medical intervention (treatment arm). In July 2015 Cambodia implemented the TCE protocol with two intervention and two control villages. Primaquine is not used in the TCE treatment regimen in Cambodia. Both studies were approved under OxTREC reference no. 1017-13 and 1015-13.

Group Type: NO_INTERVENTION

No interventions assigned to this group

Interventions

malaria elimination using DP and low-dose primaquine

Treatment of all persons resident in the intervention villages including those who do not have malaria parasites as detected by rapid diagnostic test. This is to interrupt p.f malaria transmission by removing the reservoir of all potentially infectious people from the area.

Intervention Type: DRUG

Other Intervention Names

Three monthly rounds of: Dihydroartemisinin-piperaquine; Low-dose primaquine

Eligibility Criteria

Inclusion Criteria

• Age ≥6 months, male or female,
• Written informed consent (by parent/guardian in case of children)

• Age ≥6 months, male or female,
• Written informed consent (by legally acceptable representative in case of children)
• Healthy at the time of the survey or drug administration
• Not pregnant

• Males and females 18 and above
• Written informed consent

• Age ≥one year, male and female,
• Willing to provide consent for those 18 years and above. For children 10-18 years old, parents/guardians must provide consent, and the children must provide assent. For children below 10 years old, the parents/guardians must provide consent.

Exclusion Criteria

• Pregnant women will not receive primaquine (urine pregnancy tests will be performed on women of appropriate age groups before drug administration at each TCE round)
• History of allergy or known contraindication to artemisinins, piperaquine or PQ
• Those who are, in the opinion of the study clinician, ill at the time of drug administration

OxTREC reference: 1015-13

• Significant non-compliance with study requirements
• Loss to follow up
• Suspected severe adverse events
• Severe illness

OxTREC reference: 23-15

Part 1. qPCR survey for identification of potential TMT villages;

• Pregnant women in their first trimester
• Presence of any acute severe illness at the time of survey

Part 2. TMT villages will be given directly observed therapy (DOT) with DP for 3 days and PQ (0.25 mg/kg) will be given on day 1

• History of allergy or known contraindication to artemisinins, piperaquine or PQ.
• Refusal of treatment.
• Pregnant women in their 1st trimester.

• Minimum Eligible Age: 6 Months
• Eligible Sex: ALL
• Accepts Healthy Volunteers: Yes

Sponsors

Mahidol Oxford Tropical Medicine Research Unit

OTHER

Sponsor Role: collaborator

National Centre for Parasitology, Entomology and Malaria Control, Cambodia

OTHER

Sponsor Role: collaborator

FHI 360

OTHER

Sponsor Role: collaborator

Oxford University Clinical Research Unit, Vietnam

OTHER

Sponsor Role: collaborator

National Malaria Control Program, Vietnam

OTHER_GOV

Sponsor Role: collaborator

Myanmar Oxford Clinical Research Unit

OTHER

Sponsor Role: collaborator

National Malaria Control Program, Myanmar

OTHER_GOV

Sponsor Role: collaborator

Lao-Oxford-Mahosot Hospital Wellcome Trust Research Unit

OTHER

Sponsor Role: collaborator

Shoklo Malaria Research Unit

OTHER

Sponsor Role: collaborator

University of Oxford

OTHER

Sponsor Role: lead

Responsible Party

Responsibility Role: SPONSOR

Principal Investigators

Nicholas J White, PhD

Role: PRINCIPAL_INVESTIGATOR

University of Oxford

Locations

Mahidol Oxford Clincal Research Unit, Myanmar

Rangoon, , Burma

Pailin

Pailin, , Cambodia

Savannakhet

Savannakhet, , Laos

Shoklo Malaria Research Unit

Mae Sot, Changwat Tak, Thailand

Oxford University Clinical Research Unit - Vietnam

Ho Chi Minh City, , Vietnam

Countries

Burma; Cambodia; Laos; Thailand; Vietnam

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Related Links

http://www.tropmedres.ac

The Mahidol Oxford Tropical Medicine Research Unit

Other Identifiers

BAKMAL1305

Identifier Type: -

Identifier Source: org_study_id