Nasal Mucin Oral Supplementation Treatment Respiratory Inflammation Using PUFA in Cystic Fibrosis (CF) Patients

NCT ID: NCT01329172

Last Updated: 2017-12-22

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

TERMINATED

Clinical Phase

NA

Total Enrollment

7 participants

Study Classification

INTERVENTIONAL

Study Start Date

2010-09-20

Study Completion Date

2017-10-31

Brief Summary

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Background :

Cystic fibrosis (CF) patients are prone to recurrent pulmonary infection have different secreted mucin pattern from healthy subjects. Long chain polyunsaturated fatty acids have been shown to influence survival and MUC5B expression in mice model of chronic pulmonary infection.

Method :

To study the impact of LCPUFA n-3 on MUC5B expression (mRNA level by RT-PCR) collected in airway epithelial cells obtained by nasal brushing. The secondary aim is to assess : MUC1, MUC2, MUC4, MUC5AC, MUC7 expression (mRNA level) in airway epithelial cells obtained by nasal brushing; Lund-Kennedy score; TNK-alpha, IL-6, IL-8 in blood plasma. This study is a double parallel, controlled double blind, randomized clinical trial : LCPUFA n-3 (1 g/day) vs placebo for 6 weeks. 30 subjects will be included in this study. Primary and secondary study end point will be assessed two times: before randomization and after 60 days of treatment.

Statistical analysis :

Treatment group and placebo will be compared using U-Mann-Whitney, intention to treat and per protocol.

Detailed Description

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Primary Outcome Measure : Mucin MUC5B (mRNA level) in native airway epithelial cells obtained by nasal brushing

Secondary Outcome Measures : Mucin MUC1, MUC2, MUC4, MUC5AC, MUC7 (mRNA level) in native airway epithelial cells obtained by nasal brushing.

Lund-Kennedy score TNF-alpha, IL-6, IL-8 in plasma

Conditions

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Cystic Fibrosis Dietary Modification

Keywords

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PUFA Cystic Fibrosis Nasal brushing Mucins Mucin production

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

PARALLEL

Primary Study Purpose

TREATMENT

Blinding Strategy

SINGLE

Investigators

Study Groups

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polyunsaturated fatty acids n-3

polyunsaturated fatty acids n-3 (PUFA n-3)

Group Type EXPERIMENTAL

polyunsaturated fatty acids n-3

Intervention Type OTHER

polyunsaturated fatty acids n-3 1 gram per day during two months

placebo

sun flower oil

Group Type PLACEBO_COMPARATOR

placebo

Intervention Type OTHER

sun flower oil with Poly Unsaturated Fatty Acid 1g/day

Interventions

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polyunsaturated fatty acids n-3

polyunsaturated fatty acids n-3 1 gram per day during two months

Intervention Type OTHER

placebo

sun flower oil with Poly Unsaturated Fatty Acid 1g/day

Intervention Type OTHER

Other Intervention Names

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PUFA n-3 PUFA

Eligibility Criteria

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Inclusion Criteria

* Cystic fibrosis patient
* Aged 18 to 30 year
* No modification of long term therapy (Corticoids, antibiotics, pancreatic extract, antiacid) before 4 weeks before randomization

Exclusion Criteria

* Awaiting transplantation
* Patients on anticoagulants
* Contraindication to supplementation with polyunsaturated fatty acids
* Taking antibiotics in progress ( less than 15 days)
* Taking anti- inflammatory ongoing (more than one week per month , steroids , or nonsteroidal )
Minimum Eligible Age

18 Years

Maximum Eligible Age

30 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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University Hospital, Lille

OTHER

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Frédéric GOTTRAND, MD, PhD

Role: STUDY_CHAIR

CHRU de Lille et Université Lille 2

Locations

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Dunkerque Hospital

Dunkirk, , France

Site Status

Lens Hospital

Lens, , France

Site Status

Clinical Investigation center

Lille, , France

Site Status

Countries

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France

Other Identifiers

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2009-014869-43

Identifier Type: EUDRACT_NUMBER

Identifier Source: secondary_id

2008_47/0928

Identifier Type: -

Identifier Source: org_study_id