Induction of Neutrophilic Traps ( NETs ) by the Environment and Infectious Inflammatory Lung Cystic Fibrosis
NCT ID: NCT02564393
Last Updated: 2015-09-30
Study Results
The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.
Basic Information
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COMPLETED
55 participants
OBSERVATIONAL
2011-05-31
Brief Summary
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Phase 1 : Secretion of NETs in response to lung disease and their secretion of factors.
This is a transversal study in which we compare :
* Producing NETs (DNA and proteases ) by neutrophils purified from sputum and blood of patients
* Producing NETs (DNA and proteases ) by purified neutrophils in the blood of patients and blood of control subjects
Phase 2: Mechanisms of activation of NETs secretory pathways . This phase will identify receptors (TLR ) involved in the secretion of NETs and analyze the involvement of dependent transduction pathways calcium and PKC . It will be performed on control blood neutrophils.
Phase 3: Analysis of cell death ( NETose )
This is a transversal study that will analyze :
the mode of cell death of neutrophils purified blood and sputum of patients and control blood neutrophils by flow cytometry and transmission electron microscopy .
Detailed Description
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Conditions
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Study Design
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CASE_CONTROL
PROSPECTIVE
Study Groups
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T
Control
No interventions assigned to this group
A
Adult with cystic fibrosis
No interventions assigned to this group
Eligibility Criteria
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Inclusion Criteria
* Men or women over 18
* Patients with mucoviscidose in stable condition, i.e. not having presented an acute push of bronchopulmonary attack requiring a antibiothérapie or a hospitalization for treatment of its disease during the 2 previous weeks
* Signed consent
Volunteers :
* Men or women over 18
* Without respiratory antecedent nor known respiratory pathology
* Signed consent
Exclusion Criteria
* In exclusion period of an other study
Volunteers :
* In exclusion period of an other study
* Antibiotic treatment and/or anti-inflammatory drug (AINS or corticoids) in the 2 weeks before inclusion in the study
18 Years
ALL
Yes
Sponsors
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University Hospital, Tours
OTHER
Responsible Party
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Locations
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CHRU de Tours
Tours, , France
Countries
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Other Identifiers
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PHAO09-PD / MucoNETs
Identifier Type: -
Identifier Source: org_study_id