Proteomics in Morbid Obesity After Bariatric Surgery

NCT ID: NCT01151917

Last Updated: 2014-01-30

Basic Information

• Recruitment Status: COMPLETED
• Total Enrollment: 20 participants
• Study Classification: OBSERVATIONAL
• Study Start Date: 2009-06-30
• Study Completion Date: 2012-07-31

Brief Summary

Glycemic control is rapidly restored in patients with insulin resistance after bariatric surgery, in particular after the mal-absorptive one (i.e. Bilio-pancreatic diversion, BPD). To evaluate the mechanisms allowing restoration of insulin sensitivity after BPD the investigators aimed at identifying by using a proteomic approach plasma proteins or peptides that may be involved in the remarkably fast and explicit restoration of insulin sensitivity. In addition to the unbiased proteomics approach, a selection of recognized markers for metabolic control will be measured. These efforts all aim at an increased understanding of how insulin sensitivity is regulated and may provide novel ideas of how to treat insulin resistance and type 2-diabetes.

Detailed Description

Study design The study is designed as a single-centre, observational study. The patients will undergo routine analyses usually performed before and after biliopancreatic diversion surgery (BPD), independently of the participation to this specific protocol with the only exception of meal test. Twenty morbidly obese male subjects scheduled for BPD for will be included in the study.

The inclusion and exclusion criteria are set as to create a sufficiently homogenous study population.

As outlined in the study flow chart subjects will be studied and blood samples collected at 3 weeks (+/- 1 week) and 4 days (+/- 1 day) before the day of BPD surgery (baseline-1 and baseline-2), at the day of surgery (day 0), every second day after surgery during the first week (immediate post surgery) as well as two and four weeks after surgery (post surgery-1 and post surgery-2). A deviation of +/- 2 days is allowed for the post surgery-1 and post surgery-2 visits.

All fasting samples are obtained in the morning after an overnight fast (12 h). Immediate post surgery (fasting) samples will be collected after 12 h rest from parenteral nutrition from the arm not used for nutritional substitution during the first 4 days after surgery (when parenteral nutrition is given). The immediate post surgery (fasting) samples obtained from day 5 and forward after surgery, when subjects have started to eat, will be obtained in the morning after an overnight fast (12 h). Nutrient stimulation samples will be obtained at baseline-1 and 2 and post surgery-1 and 2 after intake of a test meal. A complete list of all procedures for each visit is provided in Table 1 and an overview of all planned blood samples, including volumes and purpose is provided in Table 2. Motivations for the collection of various samples are provided under section 10.3.1 "Proteomics analysis, experimental design issues".

In case of discontinuation, additional subjects should be enrolled such that a complete set of samples from no less than 20 subjects can be obtained.

Target subject population Inclusion criteria

• Morbidly obese male with a BMI >40 kg/m2 who, for their obesity disease, are eligible for bariatric surgery and have accepted to undergo BPD
• Confirmed insulin resistance; fasting serum insulin level > 60 pmol/L
• Age 25-55 years
• Weight stable for at least 6 months before the study (+/- 5 kg within the previous 6 months)
• Stable medication
• Provision of informed consent, statistical analysis, and publications of obtained results Exclusion criteria
• Patients not eligible for BPD
• Incapacity to give a valid informed consent or unwilling to give the consent
• Patients eligible for BPD, but with:

• Type 2-diabetes mellitus
• Significant illness within the two weeks preceding surgery, as judged by the physician.
• Obvious infection (bacteria, virus etc)
• Major cardiovascular disease
• Major gastrointestinal, respiratory, or any hormonal disorders
• Medication affecting lipid metabolism within 3 months of the study
• History of drug addiction and/or alcohol use
• Suspected or confirmed poor compliance
• Exercise +/-3 times a week
• Blood donation within 12 weeks preceding screening visit

Conditions

Insulin Resistance; Proteomics; Morbid Obesity

Study Design

• Observational Model Type: CASE_ONLY
• Study Time Perspective: PROSPECTIVE

Study Groups

Bilio-pancreatic diversion

Each subject is own control

No interventions assigned to this group

Eligibility Criteria

Inclusion Criteria

• Morbidly obese male with a BMI >40 kg/m2 who, for their obesity disease, are eligible for bariatric surgery and have accepted to undergo BPD
• Confirmed insulin resistance; fasting serum insulin level > 60 pmol/L
• Age 25-55 years
• Weight stable for at least 6 months before the study (+/- 5 kg within the previous 6 months)
• Stable medication
• Provision of informed consent, statistical analysis, and publications of obtained results

Exclusion Criteria

• Patients not eligible for BPD
• Incapacity to give a valid informed consent or unwilling to give the consent
• Patients eligible for BPD, but with:

• Type 2-diabetes mellitus
• Significant illness within the two weeks preceding surgery, as judged by the physician.
• Obvious infection (bacteria, virus etc)
• Major cardiovascular disease
• Major gastrointestinal, respiratory, or any hormonal disorders
• Medication affecting lipid metabolism within 3 months of the study
• History of drug addiction and/or alcohol use
• Suspected or confirmed poor compliance
• Exercise +/-3 times a week
• Blood donation within 12 weeks preceding screening visit

• Minimum Eligible Age: 25 Years
• Maximum Eligible Age: 55 Years
• Eligible Sex: MALE
• Accepts Healthy Volunteers: No

Sponsors

Catholic University of the Sacred Heart

OTHER

Sponsor Role: lead

Responsible Party

Geltrude Mingrone

professor

Responsibility Role: PRINCIPAL_INVESTIGATOR

Principal Investigators

Geltrude Mingrone, Professor

Role: PRINCIPAL_INVESTIGATOR

Catholic University of Rome

Locations

Day Hospital of Metabolic Diseases, Catholic University

Rome, , Italy

Countries

Italy

References

Karlsson C, Wallenius K, Walentinsson A, Greasley PJ, Miliotis T, Hammar M, Iaconelli A, Tapani S, Raffaelli M, Mingrone G, Carlsson B. Identification of Proteins Associated with the Early Restoration of Insulin Sensitivity After Biliopancreatic Diversion. J Clin Endocrinol Metab. 2020 Nov 1;105(11):e4157-68. doi: 10.1210/clinem/dgaa558.

Reference Type: DERIVED

PMID: 32830851 (View on PubMed)

Other Identifiers

UCSC

Identifier Type: -

Identifier Source: org_study_id