Effect of the Molecular Weight of Oat β-glucan on Its Ability to Lower Serum Cholesterol

NCT ID: NCT00981981

Last Updated: 2011-06-21

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

PHASE2

Total Enrollment

367 participants

Study Classification

INTERVENTIONAL

Study Start Date

2008-11-30

Study Completion Date

2009-08-31

Brief Summary

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The purposes of this study were:

1. To determine if a breakfast cereal containing 3g of high molecular weight oat beta-glucan fiber would lower low-density lipoprotein (LDL) - cholesterol (the "bad" cholesterol) compared to a control cereal containing wheat fiber.
2. To determine if the LDL-cholesterol-lowering effect of oat beta-glucan fiber was reduced when the molecular weight of the fiber was reduced.

Detailed Description

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The FDA allows a health claim that oat products may reduce the risk of heart disease, based on meta-analyses showing a cholesterol-lowering effect of oat beta-glucan, if the product delivers at least a 3g daily dose of oat beta-glucan. However, not all studies have demonstrated a lowering of oat products. This may be due to variable bioactivity of the beta-glucan in the oat products. The bioactivity of oat beta-glucan is believed to depend upon its viscosity in the gut. Factors influencing viscosity include the molecular weight (MW) of the beta-glucan molecule and the amount of soluble beta-glucan in the product, which, in turn determines its concentration (C) in solution. In finished food products both MW and C can be modified by beta-glucanase enzymes present in other ingredients in the food (eg. wheat flour), processing (eg. extrusion) and storage (eg. freezing of moist products such as muffins). The effect of altering the MW and solubility of beta-glucan in foods on glycemic responses has been shown, but a role for MW and C in cholesterol lowering has not been established.

To address this issue, this study was designed with 2 primary objectives:

1. An extruded oat cereal containing 3g high-molecular weight oat β-glucan daily will reduce LDL cholesterol compared to a control wheat bran cereal.
2. A significant correlation exists between LDL cholesterol and log(C×MW), where C is the amount of soluble β-glucan in the daily dose of cereal and MW is the molecular weight of the β-glucan in the cereal.

Conditions

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Hypercholesterolemia

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

PARALLEL

Primary Study Purpose

TREATMENT

Blinding Strategy

QUADRUPLE

Participants Caregivers Investigators Outcome Assessors

Study Groups

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Control

Wheat bran cereal

Group Type PLACEBO_COMPARATOR

Wheat bran

Intervention Type DIETARY_SUPPLEMENT

21g per day of ready to eat breakfast cereal containing wheat bran with 8g of total dietary fiber and 0.5g beta-glucan.

3g high MW

Cereal containing 3g high molecular weight oat beta glucan

Group Type ACTIVE_COMPARATOR

3g high MW

Intervention Type DIETARY_SUPPLEMENT

20.2 grams per day of ready to eat cereal containing 6g total dietary fiber and 3g oat beta glucan with high molecular weight

4g medium MW

Cereal containing 4g oat beta glucan with medium molecular weight

Group Type ACTIVE_COMPARATOR

4g medium MW

Intervention Type DIETARY_SUPPLEMENT

28.5g ready to eat cereal containing 8g total dietary fiber and 4g oat beta glucan with medium molecular weight

3g medium MW

Cereal containing 3g oat beta glucan with medium molecular weight

Group Type ACTIVE_COMPARATOR

3g medium MW

Intervention Type DIETARY_SUPPLEMENT

21.1g of ready to eat cereal containing 6g total fiber and 3g oat beta glucan with a medium molecular weight

4g low MW

Cereal containing 4g oat beta glucan with low molecular weight

Group Type ACTIVE_COMPARATOR

4g low MW

Intervention Type DIETARY_SUPPLEMENT

28.7g ready to eat cereal containing 8g total dietary fiber and 4g oat beta glucan with low molecular weight

Interventions

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Wheat bran

21g per day of ready to eat breakfast cereal containing wheat bran with 8g of total dietary fiber and 0.5g beta-glucan.

Intervention Type DIETARY_SUPPLEMENT

3g high MW

20.2 grams per day of ready to eat cereal containing 6g total dietary fiber and 3g oat beta glucan with high molecular weight

Intervention Type DIETARY_SUPPLEMENT

4g medium MW

28.5g ready to eat cereal containing 8g total dietary fiber and 4g oat beta glucan with medium molecular weight

Intervention Type DIETARY_SUPPLEMENT

3g medium MW

21.1g of ready to eat cereal containing 6g total fiber and 3g oat beta glucan with a medium molecular weight

Intervention Type DIETARY_SUPPLEMENT

4g low MW

28.7g ready to eat cereal containing 8g total dietary fiber and 4g oat beta glucan with low molecular weight

Intervention Type DIETARY_SUPPLEMENT

Eligibility Criteria

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Inclusion Criteria

* body mass index 18.5 to 40.0 kg/m\^2
* no intention to lose or gain weight
* fasting total cholesterol 5.0 to 8.0 mmol/L
* fasting LDL cholesterol 3.0 to 5.0 mmol/L
* consuming diet containing \<15% energy from saturated fat

Exclusion Criteria

* use of any cholesterol-lowering drug, herbal or nutritional supplement
* regular consumption of oatmeal, oat bran or psyllium - containing cereals
* fasting serum triglycerides \>4.0mmol/L
* serum aspartate transaminase \>1.5 times upper limit of normal
* serum urea or creatinine \>1.8 times upper limit of normal
* presence of diabetes or fasting glucose \>6.9mmol/L
* presence or recent major surgical or medical event
* allergy to wheat or oats
* presence of condition or drug which alters digestion or absorption of foods
Minimum Eligible Age

35 Years

Maximum Eligible Age

70 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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CreaNutrition, AG

UNKNOWN

Sponsor Role collaborator

University of Guelph

OTHER

Sponsor Role collaborator

University of Sydney

OTHER

Sponsor Role collaborator

Laval University

OTHER

Sponsor Role collaborator

Reading Scientific Services Ltd.

INDUSTRY

Sponsor Role collaborator

University of Toronto

OTHER

Sponsor Role collaborator

Agriculture and Agri-Food Canada

OTHER_GOV

Sponsor Role collaborator

Glycemic Index Laboratories, Inc

INDUSTRY

Sponsor Role lead

Responsible Party

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Glycemic Index Laboratories, Inc.

Principal Investigators

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Thomas MS Wolever, MD, PhD

Role: PRINCIPAL_INVESTIGATOR

Glycemic Index Laboratories, Inc

Peter J Wood, PhD

Role: STUDY_DIRECTOR

Agriculture and Agri-Food Canada

Susan M Tosh, PhD

Role: STUDY_DIRECTOR

Agriculture and Agri-Food Canada

Alison L Gibbs, PhD

Role: STUDY_DIRECTOR

Department of Statistics, University of Toronto

Locations

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SUGiRS Human Nutrition Unit, School of Molecular & Microbial Biosciences, Unviersity of Sydney

Sydney, New South Wales, Australia

Site Status

Human Nutraceutical Research Unit, Department of Human Health and Nutritional Sciences, University of Guelph

Guelph, Ontario, Canada

Site Status

Glycemic Index Laboratories, Inc.

Toronto, Ontario, Canada

Site Status

Nutraceuticals and Functional Foods Institute, Faculte des science de l'agriculture et de l'alimentation, Universite Laval

Laval, Quebec, Canada

Site Status

Reading Scientific Services, Ltd (RSSL)

Reading, Berkshire, United Kingdom

Site Status

Countries

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Australia Canada United Kingdom

References

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Wolever TM, Tosh SM, Gibbs AL, Brand-Miller J, Duncan AM, Hart V, Lamarche B, Thomson BA, Duss R, Wood PJ. Physicochemical properties of oat beta-glucan influence its ability to reduce serum LDL cholesterol in humans: a randomized clinical trial. Am J Clin Nutr. 2010 Oct;92(4):723-32. doi: 10.3945/ajcn.2010.29174. Epub 2010 Jul 21.

Reference Type RESULT
PMID: 20660224 (View on PubMed)

Tosh SM, Brummer Y, Miller SS, Regand A, Defelice C, Duss R, Wolever TM, Wood PJ. Processing affects the physicochemical properties of beta-glucan in oat bran cereal. J Agric Food Chem. 2010 Jul 14;58(13):7723-30. doi: 10.1021/jf904553u.

Reference Type RESULT
PMID: 20527967 (View on PubMed)

Wolever TM, Gibbs AL, Brand-Miller J, Duncan AM, Hart V, Lamarche B, Tosh SM, Duss R. Bioactive oat beta-glucan reduces LDL cholesterol in Caucasians and non-Caucasians. Nutr J. 2011 Nov 25;10:130. doi: 10.1186/1475-2891-10-130.

Reference Type DERIVED
PMID: 22118569 (View on PubMed)

Other Identifiers

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GIL8034

Identifier Type: -

Identifier Source: org_study_id

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