Study Results
The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.
Basic Information
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COMPLETED
25 participants
OBSERVATIONAL
2025-01-05
2025-12-15
Brief Summary
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Objectives: To evaluate the technical feasibility of small RNA extraction and next-generation sequencing (NGS) from EBC and to characterise small RNA profiles at rest and after maximal exercise, using EBC as a model matrix for dynamic physiological changes.
Methods: In a pilot study conducted as part of statutory research at the Medical University of Białystok (B.SUB.25.512 and B.SUB.25.529), we analysed 22 EBC samples collected with the RTube device from 11 healthy volunteers and 3 patients with lung cancer and co-existing COPD (GOLD 3-4), at rest and after standardised maximal exercise confirmed by cardiopulmonary exercise testing (CPET). Total RNA was extracted using a commercial high-throughput RNA isolation kit designed for biological samples with low nucleic acid content, the miRNeasy Serum/Plasma Advanced Kit (Qiagen), according to the manufacturer's protocol with modifications. Small RNA libraries were generated using the high-sensitivity, low-input TrueQuant Small RNA-Seq Library Preparation Kit (GenXPro GmbH) with Unique Molecular Identifiers (UMIs) and sequenced on an Illumina platform. Reads were processed with Cutadapt and FastQC, mapped iteratively to the hg38 (Homo sapiens) reference genome using Bowtie2, aligned against an array of RNA databases, including miRNA (miRBase), tRNA (GtRNAdb), piRNA (piRNAdb), ncRNA (ENSEMBL), and cDNA (ENSEMBL) and quantified with HTSeq. Differential expression analysis (DEA) and principal component analysis (PCA) were performed with DESeq2.
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Detailed Description
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Conditions
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Study Design
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COHORT
PROSPECTIVE
Study Groups
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Exhaled breath condensate donors
athletes (22) and lung cancer (3) donors of EBC
No interventions assigned to this group
Eligibility Criteria
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Inclusion Criteria
* ability to perform spirometry
Exclusion Criteria
* absolute contradictions to CPET or spirometry
18 Years
ALL
Yes
Sponsors
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Medical University of Bialystok
OTHER
Responsible Party
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Lukasz Minarowski
MD PhD
Locations
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Department of Respiratory Physiopathology, Medical University of Bialystok
Bialystok, Podlaskie Voivodeship, Poland
Countries
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Other Identifiers
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APK.002.584.2024
Identifier Type: -
Identifier Source: org_study_id
B.SUB.25.512
Identifier Type: OTHER
Identifier Source: secondary_id
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