Safety and Efficacy of Bone Bioengineering Kit With Stem Cells for Alveolar Cleft Repair

NCT ID: NCT07305623

Last Updated: 2025-12-26

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

NOT_YET_RECRUITING

Clinical Phase

PHASE3

Total Enrollment

205 participants

Study Classification

INTERVENTIONAL

Study Start Date

2026-07-31

Study Completion Date

2027-12-31

Brief Summary

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The objective of this study is to compare the safety and efficacy of the bone tissue engineering kit-composed of 10⁶ mesenchymal stem cells derived from deciduous dental pulp combined with a collagen- and hydroxyapatite-based biomaterial-with secondary autogenous bone grafting for alveolar cleft closure in patients with cleft lip and palate.

Individuals aged 6 to 11 years with unilateral pre- and transforamen cleft lip and palate who have an indication for bone grafting will be included.

The study will be conducted in two stages. In Stage 1, patients will undergo surgery using only the bioengineering kit, and the primary outcome will be the average length of hospital stay after grafting with the engineering kit. In Stage 2, patients will be randomized to receive either the bioengineering kit treatment or the standard iliac crest bone graft surgery. In this stage, the primary outcome will be the success rate of the cleft lip and palate repair surgery, determined by the amount of bone formation in the alveolar cleft region, measured through volumetric analyses of computed tomography scans performed 12 months after grafting.

Detailed Description

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Conditions

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Cleft Lip Palate

Keywords

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bioengineering kit Stem cells

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

PARALLEL

Patients will receive the bioengineering kit composed of stem cells from deciduous teeth and biomaterial.
Primary Study Purpose

TREATMENT

Blinding Strategy

SINGLE

Outcome Assessors

Study Groups

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bioengineering kit

Patients will receive the bioengineering kit composed of stem cells from deciduous teeth and biomaterial.

Group Type EXPERIMENTAL

Bioengineering kit composed of stem cells from deciduous teeth and biomaterial.

Intervention Type COMBINATION_PRODUCT

Bone tissue engineering kit composed of 10⁶ mesenchymal stromal cells/mesenchymal stem cells (MSCs) derived from dental pulp, combined with a biomaterial consisting of hydroxyapatite and collagen (BioOss Collagen, 250 mg, Geistlich).

Iliac crest bone graft

Standard iliac crest bone graft surgery

Group Type ACTIVE_COMPARATOR

iliac crest bone graft surgery

Intervention Type PROCEDURE

standard iliac crest bone graft surgery

Interventions

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Bioengineering kit composed of stem cells from deciduous teeth and biomaterial.

Bone tissue engineering kit composed of 10⁶ mesenchymal stromal cells/mesenchymal stem cells (MSCs) derived from dental pulp, combined with a biomaterial consisting of hydroxyapatite and collagen (BioOss Collagen, 250 mg, Geistlich).

Intervention Type COMBINATION_PRODUCT

iliac crest bone graft surgery

standard iliac crest bone graft surgery

Intervention Type PROCEDURE

Eligibility Criteria

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Inclusion Criteria

* Signed Informed Consent Form (ICF) from the legal guardian;
* Signed Assent Form from the participant;
* Patients aged 6 to 11 years at the time of the secondary bone graft surgery;
* Participants diagnosed with unilateral cleft lip and palate of the pre-foramen incisive or transforamen type;
* Patients must have had mesenchymal stem cells derived from deciduous teeth collected and stored in a biobank prior to signing the Informed Consent Form (ICF);
* Patients with the maxilla previously aligned through orthodontic treatment and deemed suitable for the alveolar bone graft procedure;
* Patients who are reliable and willing to be available throughout the study, attend all required study visits, and are willing and able to comply with study procedures as necessary.

Exclusion Criteria

* Previous surgery for alveolar cleft repair;
* Presence of comorbidities (diabetes, cardiac conditions, hypertension) or allergies to latex gloves and suture materials (Catgut, Vicryl, Monocryl, Nylon, Polyester, or Polypropylene);
* Presence of palatal fistulas resulting from previous surgeries;
* Incomplete orthodontic or tomography documentation;
* Eruption of the permanent canine prior to signing the Informed Consent Form (ICF) and Assent Form (AF), and prior to undergoing the secondary alveolar bone graft surgery;
* Participants who are pregnant or breastfeeding at the time of screening or during the study.
Minimum Eligible Age

6 Years

Maximum Eligible Age

11 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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Instituto de Tecnologia do Paraná

OTHER

Sponsor Role lead

Responsible Party

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Daniela Franco Bueno

DDS, PhD

Responsibility Role PRINCIPAL_INVESTIGATOR

Locations

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Hospital Pequeno Príncipe

Curitiba, Paraná, Brazil

Site Status

Countries

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Brazil

Facility Contacts

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Gustavo Lara Achôa, DDS

Role: primary

Other Identifiers

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TECPAR_2025

Identifier Type: -

Identifier Source: org_study_id