LED Photobiomodulation Improves Radial Artery Vascular Function in Vitro

NCT ID: NCT07041697

Last Updated: 2025-06-27

Basic Information

• Recruitment Status: COMPLETED
• Clinical Phase: NA
• Total Enrollment: 30 participants
• Study Classification: INTERVENTIONAL
• Study Start Date: 2023-01-12
• Study Completion Date: 2025-02-21

Brief Summary

LED photobiomodulation therapy (PBMT) is widely recognized for its beneficial effects in several clinical conditions; however, its potential in modulating vascular function is not yet fully established. Objective: This study aimed to evaluate the application of PBMT by LED (light-emittion diode) using a combined red and near-infrared light spectrum, with a focus on improving the vascular function of the radial artery in vitro. Methodology: A total of 30 radial artery segments were studied and divided into three groups of 10 segments: the PBMT LED group combined with red and infrared light (R + IR), the red light (R) group, and the infrared light (IR) group. Prior to light irradiation at wavelengths of 650 nm and 940 nm, vascular function in these segments was pharmacologically inhibited using L-NAME. The drugs phenylephrine, acetylcholine, and sodium nitroprusside were used to determine if irradiation could restore the inhibited vascular functions. Superoxide anion levels, an important reactive oxygen specie (ROS), were measured using a chemiluminescence assay with lucigenin. The bioavailability of nitric oxide was evaluated using a Griess Reagent Kit assay, while nitric oxide production was assessed through the DAF fluorescence assay. The vascular inflammation was determined by the Intercellular Adhesion Molecule 1 (ICAM-1) expression.

Conditions

Vascular Dysfunction

Study Design

• Allocation Method: NON_RANDOMIZED
• Intervention Model: CROSSOVER
• Primary Study Purpose: PREVENTION
• Blinding Strategy: NONE

Study Groups

Photobiomodulation Using Red LED Light at 650 nm Wavelength

The three arms of the study aim to evaluate the effectiveness of different LED light spectra on vasodilation.

Group Type: EXPERIMENTAL

Light-emitting diode photobiomodulation therapy

Intervention Type: DEVICE

For the intervention, the isolated arteries were assigned to three experimental groups. The first group (red) was exposed to light at a wavelength of 650 nm for 83 seconds. The second group (infrared) received irradiation at 940 nm for 94 seconds. The third group (mixed) was irradiated with a combination of red (650 nm) and infrared (940 nm) light for 73 seconds.

Photobiomodulation Using Red LED Light at 940 nm Wavelength

The three arms of the study aim to evaluate the effectiveness of different LED light spectra on vasodilation.

Group Type: EXPERIMENTAL

Light-emitting diode photobiomodulation therapy

Intervention Type: DEVICE

For the intervention, the isolated arteries were assigned to three experimental groups. The first group (red) was exposed to light at a wavelength of 650 nm for 83 seconds. The second group (infrared) received irradiation at 940 nm for 94 seconds. The third group (mixed) was irradiated with a combination of red (650 nm) and infrared (940 nm) light for 73 seconds.

Photobiomodulation with Combined Red (650 nm) and Infrared (940 nm) LED Light

The three arms of the study aim to evaluate the effectiveness of different LED light spectra on vasodilation.

Group Type: EXPERIMENTAL

Light-emitting diode photobiomodulation therapy

Intervention Type: DEVICE

For the intervention, the isolated arteries were assigned to three experimental groups. The first group (red) was exposed to light at a wavelength of 650 nm for 83 seconds. The second group (infrared) received irradiation at 940 nm for 94 seconds. The third group (mixed) was irradiated with a combination of red (650 nm) and infrared (940 nm) light for 73 seconds.

Interventions

Light-emitting diode photobiomodulation therapy

For the intervention, the isolated arteries were assigned to three experimental groups. The first group (red) was exposed to light at a wavelength of 650 nm for 83 seconds. The second group (infrared) received irradiation at 940 nm for 94 seconds. The third group (mixed) was irradiated with a combination of red (650 nm) and infrared (940 nm) light for 73 seconds.

Intervention Type: DEVICE

Eligibility Criteria

Inclusion Criteria

• Patients undergoing coronary artery bypass grafting using the radial artery

Exclusion Criteria

• Patients with poorly compliant radial arteries or those who underwent coronary artery bypass grafting without use of the radial artery were excluded from the study

• Minimum Eligible Age: 20 Years
• Maximum Eligible Age: 60 Years
• Eligible Sex: ALL
• Accepts Healthy Volunteers: Yes

Sponsors

University of Sao Paulo

OTHER

Sponsor Role: lead

Responsible Party

André Timóteo Sapalo

Clinical Professor

Responsibility Role: PRINCIPAL_INVESTIGATOR

Locations

University of São Paulo Medical School in Ribeirão Preto

Ribeirão Preto, São Paulo, Brazil

Countries

Brazil

Provided Documents

Document Type: Study Protocol and Statistical Analysis Plan

View Document

Other Identifiers

4.829.712

Identifier Type: -

Identifier Source: org_study_id