Performance Study of the Cobas® BV/CV Test on Samples From Participants With and Without Symptoms of Bacterial Vaginosis and Candida Vaginitis

NCT ID: NCT06975436

Last Updated: 2025-07-14

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Total Enrollment

738 participants

Study Classification

OBSERVATIONAL

Study Start Date

2024-12-30

Study Completion Date

2025-06-30

Brief Summary

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In the clinical performance part of this study, prospectively acquired clinician-collected and clinician-instructed, self-collected vaginal swab specimens collected in cobas® PCR Media will be taken from a minimum of 500 symptomatic individuals with a clinical presentation consistent with vaginitis, vaginosis, or both. Additionally, a minimum of 100 asymptomatic individuals will also be enrolled in the study. The cobas® BV/CV assay amplifies and detects the deoxyribonucleic acid (DNA) of pathogens associated with bacterial vaginosis (BV) and candida vaginitis (CV). The BV results will be compared with the patient infection status (PIS) established by using 3 Food and Drug Administration (FDA)-cleared commercial assays, and the CV results will be compared with the PIS established with the use of culture plus MALDI-TOF (matrix-assisted laser desorption/ionization time-of-flight).

The primary objective of the clinical performance study is to evaluate the performance (sensitivity and specificity) of cobas® BV/CV to determine the presence of BV and/or CV in the intended use patient population when being tested on cobas® 6800/8800 systems. The secondary objective is to evaluate the equivalency of cobas® BV/CV between the cobas® 5800 system and cobas® 6800/8800 systems.

Detailed Description

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Conditions

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Bacterial Vaginosis Candida Vaginitis

Study Design

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Observational Model Type

COHORT

Study Time Perspective

PROSPECTIVE

Study Groups

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Symptomatic Participants

cobas® BV/CV test

Intervention Type DIAGNOSTIC_TEST

The cobas® BV/CV test is an automated, qualitative in vitro real-time PCR diagnostic test that detects bacteria and yeast associated with bacterial vaginosis (BV) and candida vaginitis (CV), respectively. The test does not distinguish among the pathogens but can give an overall qualitative result indicating the presence or absence of pathogen(s) associated with BV and/or CV.

BD Max™ Vaginal Panel

Intervention Type DIAGNOSTIC_TEST

This is an FDA-cleared diagnostic test that will be used as labelled. It will serve as one of three comparators in this study for determining BV patient infection status.

Cepheid Xpert Xpress MVP

Intervention Type DIAGNOSTIC_TEST

This is an FDA-cleared diagnostic test that will be used as labelled. It will serve as one of three comparators in this study for determining BV patient infection status.

Hologic Aptima BV Assay

Intervention Type DIAGNOSTIC_TEST

This is an FDA-cleared diagnostic test that will be used as labelled. It will serve as one of three comparators in this study for determining BV patient infection status.

Asymptomatic Participants

cobas® BV/CV test

Intervention Type DIAGNOSTIC_TEST

The cobas® BV/CV test is an automated, qualitative in vitro real-time PCR diagnostic test that detects bacteria and yeast associated with bacterial vaginosis (BV) and candida vaginitis (CV), respectively. The test does not distinguish among the pathogens but can give an overall qualitative result indicating the presence or absence of pathogen(s) associated with BV and/or CV.

Interventions

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cobas® BV/CV test

The cobas® BV/CV test is an automated, qualitative in vitro real-time PCR diagnostic test that detects bacteria and yeast associated with bacterial vaginosis (BV) and candida vaginitis (CV), respectively. The test does not distinguish among the pathogens but can give an overall qualitative result indicating the presence or absence of pathogen(s) associated with BV and/or CV.

Intervention Type DIAGNOSTIC_TEST

BD Max™ Vaginal Panel

This is an FDA-cleared diagnostic test that will be used as labelled. It will serve as one of three comparators in this study for determining BV patient infection status.

Intervention Type DIAGNOSTIC_TEST

Cepheid Xpert Xpress MVP

This is an FDA-cleared diagnostic test that will be used as labelled. It will serve as one of three comparators in this study for determining BV patient infection status.

Intervention Type DIAGNOSTIC_TEST

Hologic Aptima BV Assay

This is an FDA-cleared diagnostic test that will be used as labelled. It will serve as one of three comparators in this study for determining BV patient infection status.

Intervention Type DIAGNOSTIC_TEST

Eligibility Criteria

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Inclusion Criteria

* Symptomatic participants with a clinical presentation consistent with vaginitis, vaginosis, or both; symptoms may include abnormal vaginal discharge, painful or frequent urination, vaginal itching or burning or irritation, painful or uncomfortable intercourse, and/or abnormal vaginal odor.
* Participants aged 14 years or older who are willing and able to provide written, informed consent; for pediatric participants, written assent and parent/legal guardian consent, as applicable by the institutional review board/ethics committee (IRB/EC).


* Apparently healthy participants aged 14 years or older who are willing and able to provide written, informed consent; for pediatric participants, written assent and parent/legal guardian consent, as applicable by the IRB/EC.

* Use of any azole-containing antimicrobial (oral or vaginal) within the 7 days prior to study enrollment.
* Prior enrollment in this study.
* Use of any lubricants (eg, Replens, RepHresh) within 3 days prior to sample collection
* Use of douches, vaginal deodorizers, or other intravaginal products within 3 days prior to sample collection. The use of tampons or pads during menses should not be considered exclusionary criteria.
* Contraindication to vaginal swab sampling.
* Asymptomatic participants who do not have any signs or symptoms consistent with vaginitis, vaginosis, or both, as described above.

Exclusion Criteria

* Prior enrollment in this study.
* Participants with a clinical presentation consistent with vaginitis, vaginosis, or both; symptoms may include abnormal vaginal discharge, painful or frequent urination, vaginal itching or burning or irritation, painful or uncomfortable intercourse, and/or abnormal vaginal odor.
Minimum Eligible Age

14 Years

Eligible Sex

FEMALE

Accepts Healthy Volunteers

Yes

Sponsors

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Roche Molecular Systems

UNKNOWN

Sponsor Role collaborator

Hoffmann-La Roche

INDUSTRY

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Clinical Trials

Role: STUDY_DIRECTOR

Hoffmann-La Roche

Locations

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Matrix Clinical Research

Los Angeles, California, United States

Site Status

M3 Wake Research - San Diego at Convoy

San Diego, California, United States

Site Status

Red Rocks OBGYN

Lakewood, Colorado, United States

Site Status

Altus Research

Lake Worth, Florida, United States

Site Status

Washington University

St Louis, Missouri, United States

Site Status

Eastern Carolina Women's Center

New Bern, North Carolina, United States

Site Status

Unified Women's Clinical Research/Lyndhurst

Winston-Salem, North Carolina, United States

Site Status

Planned Parenthood Gulf Coast

Houston, Texas, United States

Site Status

Diagnostic Consultative Center "Ascendent" Ltd.

Sofia, , Bulgaria

Site Status

Centre Hospitalier Universitaire Vaudois (Chuv)

Lausanne, , Switzerland

Site Status

Countries

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United States Bulgaria Switzerland

Other Identifiers

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RD006770

Identifier Type: -

Identifier Source: org_study_id

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