Evaluation of the PreCursor-M+® in CIN2

NCT ID: NCT06403618

Last Updated: 2025-05-23

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

RECRUITING

Total Enrollment

100 participants

Study Classification

OBSERVATIONAL

Study Start Date

2024-06-17

Study Completion Date

2027-06-17

Brief Summary

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The goal of this observational study is to to evaluate the accuracy and sensitivity of PreCursor-M+ on a post-aliquot of liquid-based cytology (LBC) cervical samples (biopsy) obtained by physicians in a group of women with histologically-proven diagnoses of CIN2.

The PreCursor-M+® assay is a multiplex real-time methylation specific PCR test that identifies the level of promotor methylation of the host cell genes FAM19A4 and miR124-2, known biomarkers associated with cervical carcinoma and transforming CIN in cervical cells.

To evaluate the clinical course of CIN2 at 2 years after the first diagnosis, with an interval evaluation at 6 months.

After enrolment, women will be divided into two groups: "active surveillance" and "immediate treatment". In the first group, clinical outcomes to be assessed, in relation to the PreCursor-M+ result at baseline, will include regression to \<CIN2, persistence of CIN2, and progression to CIN3+. In the second group, we will evaluate the histological diagnosis at cone specimen (downgrading or upgrading) and the 2-year cumulative incidence of CIN2+ recurrence based on the PreCursor-M+ result at baseline.

Detailed Description

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Conditions

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Cervical Intraepithelial Neoplasia Grade 2

Study Design

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Observational Model Type

CASE_ONLY

Study Time Perspective

PROSPECTIVE

Study Groups

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active surveillance

wait-and-see strategy

No interventions assigned to this group

immediate treatment

surgical treatment per clinical practice

No interventions assigned to this group

Eligibility Criteria

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Inclusion Criteria

1. age at diagnosis of 18 years or older;
2. histological confirmation of CIN2 after colposcopic-guided cervical biopsy or conservative surgical treatment, including loop electrosurgical excision procedure (LEEP) and laser conization;
3. known HPV test result at baseline;
4. ability to understand and sign the informed consent;
5. written informed consent given.

Exclusion Criteria

1. unknown HPV test result at diagnosis;
2. vulnerable patients.
Minimum Eligible Age

18 Years

Eligible Sex

FEMALE

Accepts Healthy Volunteers

No

Sponsors

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European Institute of Oncology

OTHER

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Anna Daniela Iacobone

Role: PRINCIPAL_INVESTIGATOR

European Institute of Oncology

Locations

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European Institute of Oncology, Milan,

Milan, , Italy

Site Status RECRUITING

European Institute of Oncology

Milan, , Italy

Site Status NOT_YET_RECRUITING

Countries

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Italy

Central Contacts

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Anna Daniela Iacobone

Role: CONTACT

+39 0294371088

Facility Contacts

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ANNA DANIELA IACOBONE

Role: primary

+39 0294371088

Anna Daniela Iacobone

Role: primary

Other Identifiers

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UID 4438

Identifier Type: -

Identifier Source: org_study_id

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