A Phase 2 Study Evaluating Safety and Tolerability of RCT2100 (CFTR mRNA) in Healthy Participants and in Participants With CF

NCT ID: NCT06237335

Last Updated: 2026-01-16

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

RECRUITING

Clinical Phase

PHASE2

Total Enrollment

192 participants

Study Classification

INTERVENTIONAL

Study Start Date

2024-02-01

Study Completion Date

2026-12-31

Brief Summary

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This is the first-in-human study with RCT2100 and is designed to provide safety and tolerability data for future clinical studies.

Detailed Description

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This is a multi-part study to assess the safety, tolerability, and biodistribution of a single ascending dose of inhaled RCT2100 administered via nebulizer to healthy participants (Part 1), the safety and tolerability of multiple-ascending doses of inhaled RCT2100 administered to participants with CF (Part 2), and the safety and tolerability of RCT2100 co-administered with ivacaftor in participants with CF (Part 3).

Conditions

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Cystic Fibrosis

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

PARALLEL

Primary Study Purpose

TREATMENT

Blinding Strategy

DOUBLE

Participants Investigators
Participant and investigator masking only applies to Part 1 which is randomized. For Part 2, there is no masking, and this part is Open Label.

Study Groups

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RCT2100 (Part 1)

RCT2100 single dose

Group Type EXPERIMENTAL

RCT2100

Intervention Type DRUG

RCT2100 supplied as varying dose strengths administered via oral inhalation using nebulizer

Placebo (Part 1)

Placebo single dose

Group Type PLACEBO_COMPARATOR

Placebo

Intervention Type OTHER

Placebo of similar volumes to experimental dose strengths administered via oral inhalation using nebulizer

RCT2100 (Part 2) 4 week

RCT2100 multiple dose

Group Type EXPERIMENTAL

RCT2100

Intervention Type DRUG

RCT2100 supplied as varying dose strengths administered via oral inhalation using nebulizer for 4 weeks

RCT2100 (Part 2) 12 week

RCT2100 multiple dose

Group Type EXPERIMENTAL

RCT2100

Intervention Type DRUG

RCT2100 supplied at a single dose strength administered via oral inhalation using nebulizer for 12 weeks

Experimental: RCT2100 (Part 3) 6 week

RCT2100 multiple dose

Group Type EXPERIMENTAL

Ivacaftor

Intervention Type DRUG

ivacaftor administered orally for 6 weeks

RCT2100

Intervention Type DRUG

RCT2100 supplied at varying dose strengths. Co- administered via oral inhalation using nebulizer for 4 weeks with ivacaftor after initial 2 weeks of ivacaftor dosing run in period

Interventions

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RCT2100

RCT2100 supplied as varying dose strengths administered via oral inhalation using nebulizer

Intervention Type DRUG

Placebo

Placebo of similar volumes to experimental dose strengths administered via oral inhalation using nebulizer

Intervention Type OTHER

RCT2100

RCT2100 supplied as varying dose strengths administered via oral inhalation using nebulizer for 4 weeks

Intervention Type DRUG

RCT2100

RCT2100 supplied at a single dose strength administered via oral inhalation using nebulizer for 12 weeks

Intervention Type DRUG

Ivacaftor

ivacaftor administered orally for 6 weeks

Intervention Type DRUG

RCT2100

RCT2100 supplied at varying dose strengths. Co- administered via oral inhalation using nebulizer for 4 weeks with ivacaftor after initial 2 weeks of ivacaftor dosing run in period

Intervention Type DRUG

Eligibility Criteria

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Inclusion Criteria

* Healthy, adult, male or female, 18-55 years of age, inclusive, at screening.
* Body weight greater than or equal to 50 kg and body mass index (BMI) between 16-32 kg/m2, inclusive
* The participant has a forced expiratory volume in one second (FEV1) of at least 80% predicted
* The participant is considered by the investigator to be in good general health as determined by medical history, clinical laboratory test results, vital sign measurements, 12-lead ECG results, and physical examination findings at screening.
* Understands the study procedures in the informed consent form (ICF), and is willing and able to comply with the protocol.


* Confirmed diagnosis of CF
* Forced expiratory volume in 1 second ≥50% and ≤100% of predicted mean value for age, sex, and height
* a) Not eligible for CFTR modulators based on having mutations of CFTR gene on both alleles that are not responsive to CFTR modulator therapy OR
* b) Eligible for CFTR modulators (based on local prescribing information) but not using CFTR modulators due to intolerance or contraindications


* Confirmed diagnosis of CF
* Forced expiratory volume in 1 second ≥50% and ≤100% of predicted mean value for age, sex, and height
* a) Not eligible for CFTR modulators based on having mutations of CFTR gene on both alleles that are not responsive to CFTR modulator therapy OR
* b) Eligible for dual or triple CFTR modulators (based on local prescribing information) but not using CFTR modulators due to intolerance or contraindications

Exclusion Criteria

* History or presence of clinically significant medical, surgical, clinical laboratory, or psychiatric condition or disease.
* The participant has supine blood pressure (BP) \>150 mm Hg (systolic) or \>90 mm Hg (diastolic), following at least 5 minutes of supine rest.
* The participant has abnormal clinical laboratory tests at screening, as assessed by the study-specific laboratory.
* The participant is a smoker or has used nicotine or nicotine-containing products 6 weeks before the first dose of study drug. Former smokers with greater than 10 pack years of smoking history are excluded.


* Hepatic cirrhosis with portal hypertension, moderate hepatic impairment (Child Pugh Score 7 to 9), or severe hepatic impairment (Child Pugh Score 10 to 15)
* An acute upper or lower respiratory infection, pulmonary exacerbation, or changes in therapy (including antibiotics) for sinopulmonary disease within 4 weeks before the first dose of study drug
* Lung infection with organisms associated with a more rapid decline in pulmonary status
* Arterial oxygen saturation on room air less than 94% at screening
* Treatment with a CFTR modulator (Kalydeco, Trikafta, Symdeko, Orkambi, or Alyftrek) within 12 weeks of Screening



* Hepatic cirrhosis with portal hypertension, moderate hepatic impairment (Child Pugh Score 7 to 9), or severe hepatic impairment (Child Pugh Score 10 to 15)
* An acute upper or lower respiratory infection, pulmonary exacerbation, or changes in therapy (including antibiotics) for sinopulmonary disease within 4 weeks before the first dose of study drug
* Lung infection with organisms associated with a more rapid decline in pulmonary status
* Arterial oxygen saturation on room air less than 94% at screening
* Treatment with a CFTR modulator (Kalydeco, Trikafta, Symdeko, Orkambi, or Alyftrek) within 12 weeks of Screening
Minimum Eligible Age

18 Years

Maximum Eligible Age

60 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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ReCode Therapeutics

INDUSTRY

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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John Matthews, MBBS, MCRP, PhD

Role: STUDY_CHAIR

ReCode Therapeutics, Inc.

Locations

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The University of Alabama at Birmingham

Birmingham, Alabama, United States

Site Status RECRUITING

University of Arizona

Tucson, Arizona, United States

Site Status RECRUITING

Stanford University

Palo Alto, California, United States

Site Status RECRUITING

UCSD

San Diego, California, United States

Site Status RECRUITING

National Jewish Health

Denver, Colorado, United States

Site Status RECRUITING

Emory University

Atlanta, Georgia, United States

Site Status RECRUITING

Boston Children's Hospital

Boston, Massachusetts, United States

Site Status RECRUITING

New York Medical College

Valhalla, New York, United States

Site Status RECRUITING

The University of North Carolina at Chapel Hill

Chapel Hill, North Carolina, United States

Site Status RECRUITING

Oregon Health & Science University

Portland, Oregon, United States

Site Status RECRUITING

University of Pittsburgh

Pittsburgh, Pennsylvania, United States

Site Status RECRUITING

UT Southwestern Medical Center

Dallas, Texas, United States

Site Status RECRUITING

University of Washington

Seattle, Washington, United States

Site Status RECRUITING

Centre Hospitalier Régional Universitaire de Montpellier - Hôpital Arnaud de Villeneuve

Montpellier, , France

Site Status RECRUITING

Hôpital Necker Enfants Malades

Paris, , France

Site Status RECRUITING

UMC Utrecht

Utrecht, , Netherlands

Site Status RECRUITING

New Zealand Clinical Research (Part 1 Only)

Auckland, , New Zealand

Site Status COMPLETED

University Hospitals Birmingham

Birmingham, , United Kingdom

Site Status RECRUITING

Royal Papworth Hospital

Cambridge, , United Kingdom

Site Status RECRUITING

Leeds Teaching Hospitals

Leeds, , United Kingdom

Site Status RECRUITING

King's College Hospital

London, , United Kingdom

Site Status RECRUITING

Nottingham University Hospitals

Nottingham, , United Kingdom

Site Status RECRUITING

University Hospital Southampton

Southampton, , United Kingdom

Site Status RECRUITING

Countries

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United States France Netherlands New Zealand United Kingdom

Central Contacts

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Priya Ryali, MBA

Role: CONTACT

650-629-7900

Other Identifiers

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2024-512169-15

Identifier Type: EUDRACT_NUMBER

Identifier Source: secondary_id

RCT2100-101

Identifier Type: -

Identifier Source: org_study_id

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