Trial of Novel Regimens for the Treatment of Pulmonary Tuberculosis

NCT ID: NCT06192160

Last Updated: 2025-11-12

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

RECRUITING

Clinical Phase

PHASE2

Total Enrollment

315 participants

Study Classification

INTERVENTIONAL

Study Start Date

2025-03-11

Study Completion Date

2027-08-11

Brief Summary

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A5409/RAD-TB is an adaptive Phase 2 randomized, controlled, open-label, dose-ranging, platform protocol to evaluate the safety and efficacy of multidrug regimens for the treatment of adults with drug-susceptible pulmonary tuberculosis (TB).

A5409 hypothesizes that novel regimens for the treatment of pulmonary tuberculosis will result in superior early efficacy, as determined by longitudinal mycobacteria growth indicator tube (MGIT) liquid culture time to positivity (TTP) measurements over the first 6 weeks of treatment, and will have acceptable safety and tolerability over 8 weeks of treatment relative to standard of care \[(SOC) isoniazid/rifampicin/pyrazinamide/ethambutol (HRZE)\].

The study will run for 52 weeks, inclusive of 26 weeks of TB treatment comprised of 8 weeks of experimental or SOC treatment (based on treatment arm assignment) followed by 18 weeks of SOC treatment with 45 participants in each experimental treatment arm and at least 90 participants in the SOC arm.

Detailed Description

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Conditions

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Pulmonary Tuberculosis

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

PARALLEL

Primary Study Purpose

TREATMENT

Blinding Strategy

NONE

Study Groups

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Arm 3A: BDQ, Pa and TBI-223 (1200 mg)

* Weeks 1-8: BDQ 400 mg for 2 weeks and then 200 mg for 6 weeks, Pa 200 mg, TBI-223 1200 mg
* Weeks 9-26: INH 300 mg, RIF 600 mg

Group Type EXPERIMENTAL

Isoniazid

Intervention Type DRUG

INH 300 mg will be administered as one tablet orally once daily.

Rifampicin

Intervention Type DRUG

RIF 600 mg will be administered as two 300 mg capsules orally once daily on an empty stomach, 1 hour before or 2 hours after eating a meal.

Bedaquiline

Intervention Type DRUG

BDQ 400 mg will be administered as four 100 mg tablets orally once daily with a meal for the first 2 weeks followed by 200 mg (two 100 mg tablets) orally once daily with a meal for 6 weeks.

Pretomanid

Intervention Type DRUG

Pa 200 mg will be administered as one 200 mg tablet orally once daily with a meal.

TBI-223

Intervention Type DRUG

TBI-223 1200 mg once daily will be administered as two 600 mg tablets orally with a meal.

Arm 3B: BDQ, Pa and TBI-223 (2400 mg)

* Weeks 1-8: BDQ 400 mg for 2 weeks and then 200 mg for 6 weeks, Pa 200 mg, TBI-223 2400 mg
* Weeks 9-26: INH 300 mg, RIF 600 mg

Group Type EXPERIMENTAL

Isoniazid

Intervention Type DRUG

INH 300 mg will be administered as one tablet orally once daily.

Rifampicin

Intervention Type DRUG

RIF 600 mg will be administered as two 300 mg capsules orally once daily on an empty stomach, 1 hour before or 2 hours after eating a meal.

Bedaquiline

Intervention Type DRUG

BDQ 400 mg will be administered as four 100 mg tablets orally once daily with a meal for the first 2 weeks followed by 200 mg (two 100 mg tablets) orally once daily with a meal for 6 weeks.

Pretomanid

Intervention Type DRUG

Pa 200 mg will be administered as one 200 mg tablet orally once daily with a meal.

TBI-223

Intervention Type DRUG

TBI-223 2400 mg once daily will be administered as four 600 mg tablets orally once daily with a meal.

Arm 4A: BDQ, Pa and Sutezolid (SZD) (800 mg)

* Weeks 1-8: BDQ 400 mg for 2 weeks and then 200 mg for 6 weeks, Pa 200 mg, SZD 800 mg
* Weeks 9-26: INH 300 mg, RIF 600 mg

Group Type EXPERIMENTAL

Isoniazid

Intervention Type DRUG

INH 300 mg will be administered as one tablet orally once daily.

Rifampicin

Intervention Type DRUG

RIF 600 mg will be administered as two 300 mg capsules orally once daily on an empty stomach, 1 hour before or 2 hours after eating a meal.

Bedaquiline

Intervention Type DRUG

BDQ 400 mg will be administered as four 100 mg tablets orally once daily with a meal for the first 2 weeks followed by 200 mg (two 100 mg tablets) orally once daily with a meal for 6 weeks.

Pretomanid

Intervention Type DRUG

Pa 200 mg will be administered as one 200 mg tablet orally once daily with a meal.

Sutezolid

Intervention Type DRUG

SZD 800 mg once daily will be administered as two 400 mg tablets orally once daily with a meal.

Arm 4B: BDQ, Pa and SZD (1600 mg)

* Weeks 1-8: BDQ 400 mg for 2 weeks and then 200 mg for 6 weeks, Pa 200 mg, SZD 1600 mg
* Weeks 9-26: INH 300 mg, RIF 600 mg

Group Type EXPERIMENTAL

Isoniazid

Intervention Type DRUG

INH 300 mg will be administered as one tablet orally once daily.

Rifampicin

Intervention Type DRUG

RIF 600 mg will be administered as two 300 mg capsules orally once daily on an empty stomach, 1 hour before or 2 hours after eating a meal.

Bedaquiline

Intervention Type DRUG

BDQ 400 mg will be administered as four 100 mg tablets orally once daily with a meal for the first 2 weeks followed by 200 mg (two 100 mg tablets) orally once daily with a meal for 6 weeks.

Pretomanid

Intervention Type DRUG

Pa 200 mg will be administered as one 200 mg tablet orally once daily with a meal.

Sutezolid

Intervention Type DRUG

SZD 1600 mg once daily will be administered as four 400 mg tablets orally once daily with a meal.

Arm 1: Standard of Care (SOC)

* Weeks 1-8: INH 300 mg, RIF 600 mg, PZA weight-based, EMB weight-based
* Weeks 9-26: INH 300 mg, RIF 600 mg

Group Type ACTIVE_COMPARATOR

Isoniazid

Intervention Type DRUG

INH 300 mg will be administered as one tablet orally once daily.

Rifampicin

Intervention Type DRUG

RIF 600 mg will be administered as two 300 mg capsules orally once daily on an empty stomach, 1 hour before or 2 hours after eating a meal.

Pyrazinamide

Intervention Type DRUG

PZA will be administered as 500 mg tablets, based on weight, orally once daily.

Ethambutol

Intervention Type DRUG

EMB will be administered as 400 mg tablets, based on weight, orally once daily.

Arm 2: Bedaquiline (BDQ), Pretomanid (Pa), and Linezolid (LZD)

* Weeks 1-8: BDQ 400 mg for 2 weeks and then 200 mg for 6 weeks, Pa 200 mg, LZD 600 mg
* Weeks 9-26: INH 300 mg, RIF 600 mg

Group Type EXPERIMENTAL

Isoniazid

Intervention Type DRUG

INH 300 mg will be administered as one tablet orally once daily.

Rifampicin

Intervention Type DRUG

RIF 600 mg will be administered as two 300 mg capsules orally once daily on an empty stomach, 1 hour before or 2 hours after eating a meal.

Bedaquiline

Intervention Type DRUG

BDQ 400 mg will be administered as four 100 mg tablets orally once daily with a meal for the first 2 weeks followed by 200 mg (two 100 mg tablets) orally once daily with a meal for 6 weeks.

Pretomanid

Intervention Type DRUG

Pa 200 mg will be administered as one 200 mg tablet orally once daily with a meal.

Linezolid

Intervention Type DRUG

LZD 600 mg will be administered as one 600 mg tablet orally once daily.

Interventions

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Isoniazid

INH 300 mg will be administered as one tablet orally once daily.

Intervention Type DRUG

Rifampicin

RIF 600 mg will be administered as two 300 mg capsules orally once daily on an empty stomach, 1 hour before or 2 hours after eating a meal.

Intervention Type DRUG

Pyrazinamide

PZA will be administered as 500 mg tablets, based on weight, orally once daily.

Intervention Type DRUG

Ethambutol

EMB will be administered as 400 mg tablets, based on weight, orally once daily.

Intervention Type DRUG

Bedaquiline

BDQ 400 mg will be administered as four 100 mg tablets orally once daily with a meal for the first 2 weeks followed by 200 mg (two 100 mg tablets) orally once daily with a meal for 6 weeks.

Intervention Type DRUG

Pretomanid

Pa 200 mg will be administered as one 200 mg tablet orally once daily with a meal.

Intervention Type DRUG

Linezolid

LZD 600 mg will be administered as one 600 mg tablet orally once daily.

Intervention Type DRUG

TBI-223

TBI-223 2400 mg once daily will be administered as four 600 mg tablets orally once daily with a meal.

Intervention Type DRUG

Sutezolid

SZD 1600 mg once daily will be administered as four 400 mg tablets orally once daily with a meal.

Intervention Type DRUG

TBI-223

TBI-223 1200 mg once daily will be administered as two 600 mg tablets orally with a meal.

Intervention Type DRUG

Sutezolid

SZD 800 mg once daily will be administered as two 400 mg tablets orally once daily with a meal.

Intervention Type DRUG

Other Intervention Names

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INH RIF PZA EMB BDQ Pa LZD SZD SZD

Eligibility Criteria

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Inclusion Criteria

1. Pulmonary TB (among individuals either without history of prior TB treatment or with history of TB treatment more than 5 years prior to study entry), identified within 7 days prior to study entry by at least one sputum specimen positive for Mtb by Xpert. Semiquantitative Mtb results of "medium" or "high" are required.
2. Pulmonary TB with documented INH susceptibility (by Line Probe Assay (LPA) or Xpert MTB/XDR or other validated molecular test) and with documented RIF susceptibility (by LPA or Xpert MTB/RIF or Xpert MTB/RIF Ultra or other validated molecular test) within 7 days prior to study entry.
3. Documentation of HIV-1 infection status, as below:

* Presence or Absence of HIV-1 infection, as documented by:

* Any licensed rapid HIV test or HIV-1 enzyme or chemiluminescence immunoassay (E/CIA) test kit, any time prior to study entry. AND
* Confirmed by one of the following:

* A second antibody test from different manufacturers or based on different principles and epitopes (combination antigen-antibody-based rapid tests may be used), or
* HIV-1 antigen, or
* Plasma HIV-1 RNA viral load, or
* A licensed Western blot
4. For individuals with HIV: CD4+ cell count ≥100 cells/mm3 based on testing performed within 30 days prior to study entry.
5. For individuals with HIV: Currently being treated with dolutegravir-based antiretroviral therapy (ART), or plan to initiate dolutegravir-based ART at or before study week 8.
6. Individuals age ≥18 years.
7. The following laboratory values obtained within 7 days prior to study entry at any network-approved non-US laboratory that operates in accordance with Good Clinical Laboratory Practices (GCLP) and participates in appropriate external quality assurance programs:

* Serum or plasma alanine aminotransferase (ALT) ≤3 times the upper limit of normal (ULN)
* Serum or plasma total bilirubin ≤2 times ULN
* Serum or plasma creatinine ≤2 times ULN
* Serum or plasma potassium ≥3.5 mEq/L
* Serum or plasma magnesium ≥1.0 mEq/L (≥0.500 mmol/L)
* Absolute neutrophil count (ANC) ≥1500/mm\^3
* Hemoglobin ≥9.5 g/dL for individuals assigned to female sex at birth and ≥10.0 g/dL for individuals assigned to male sex at birth
* Platelet count ≥100,000/mm\^3
* Negative for hepatitis B core antibody (HBcAb) total, hepatitis B surface antigen (HBsAg)
* Negative for hepatitis C virus (HCV) antibody (or if HCV antibody positive, must have a negative HCV PCR)
8. For individuals assigned to female sex at birth and who are of reproductive potential, negative pregnancy test (urine HCG or serum β-HCG) within 3 days (72 hours) prior to entry by any network-approved non-US laboratory or clinic that operates in accordance with GCLP and participates in appropriate external quality assurance programs.

Individuals assigned to female sex at birth, who are of reproductive potential, and who participate in sexual activity that could lead to pregnancy must agree to use at least two of the following forms of birth control while receiving TB study medications and for 12 months after stopping study medications:
* Male or female condoms
* Diaphragm or cervical cap (with spermicide, if available)
* Intrauterine device (IUD) or intrauterine system (IUS)
* Hormone-based birth control (e.g., oral contraceptives, Depo-Provera, NuvaRing, implants)
9. For individuals who are assigned male sex at birth who engage in sexual activity that may lead to pregnancy in their partner must agree to either remain abstinent or use male contraceptives. They are also strongly advised to inform their non-pregnant sexual partners of reproductive potential to use effective contraceptives while the individual is on study and for 90 days after experimental treatment discontinuation.
10. For individuals assigned male sex at birth with pregnant partners, willingness to use condoms during vaginal intercourse while on study and for 90 days after experimental treatment discontinuation.
11. For individuals assigned male sex at birth, willingness to refrain from sperm donation while on study and for 90 days after experimental treatment discontinuation.
12. Documentation of Karnofsky performance score ≥60 obtained within 14 days prior to study entry.
13. Chest x-ray obtained within 14 days prior to study entry.
14. A verifiable address or residence readily accessible for visiting, and willingness to inform the study team of any change of address during study treatment and follow-up period.
15. Ability and willingness of individual to provide informed consent.

Exclusion Criteria

1. More than cumulative 7 days of treatment directed against active TB for the current TB episode in the 60 days preceding study entry.
2. Current extrapulmonary TB, in the opinion of the investigator.
3. QTcF interval \>450 ms within 7 days prior to study entry.
4. History of or ongoing heart failure.
5. Personal or family history of congenital QT prolongation.
6. History of known, untreated, ongoing hypothyroidism.
7. History of or ongoing bradyarrhythmia.
8. History of torsades de pointes.
9. Current Grade 2 or higher peripheral neuropathy.
10. Other medical conditions (e.g., diabetes, liver or kidney disease, blood disorders, chronic diarrhea), in the opinion of the site investigator, in which the current clinical condition of the participant is likely to prejudice the response to, or assessment of, treatment.
11. Pregnant or breastfeeding or planning to become pregnant within the next 12 months.
12. Weight \<35 kg.
13. Unable to take oral medications.
14. Taking any of prohibited medications.
15. Known allergy/sensitivity or any hypersensitivity to components of investigational agents or their formulation.
16. Active drug or alcohol use or dependence; or mental illness (e.g., major depression) that, in the opinion of the site investigator, would interfere with adherence to study requirements.
17. Taking an investigational drug or vaccine within 30 or more days prior to study entry.
Minimum Eligible Age

18 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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TB Alliance

UNKNOWN

Sponsor Role collaborator

National Institute of Allergy and Infectious Diseases (NIAID)

NIH

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Radojka Savic, PharmD, PhD

Role: STUDY_CHAIR

University of California

Kelly Dooley, MD, PhD

Role: STUDY_CHAIR

Vanderbilt University Medical Center

Gustavo Velásquez, MD, MPH

Role: STUDY_CHAIR

University of California

Locations

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12701, Gaborone CRS

Gaborone, , Botswana

Site Status NOT_YET_RECRUITING

12201, Hospital Nossa Senhora da Conceicao CRS

Porto Alegre, , Brazil

Site Status NOT_YET_RECRUITING

12101, Instituto de Pesquisa Clinica Evandro Chagas (IPEC) CRS

Rio de Janeiro, , Brazil

Site Status RECRUITING

30022, Les Centres GHESKIO Clinical Research Site (GHESKIO-INLR) CRS

Port-au-Prince, , Haiti

Site Status NOT_YET_RECRUITING

31730, GHESKIO Institute of Infectious Diseases and Reproductive Health (GHESKIO - IMIS) CRS

Port-au-Prince, , Haiti

Site Status NOT_YET_RECRUITING

31441, Byramjee Jeejeebhoy Medical College (BJMC) CRS

Pune, , India

Site Status NOT_YET_RECRUITING

12601, Moi University Clinical Research Center (MUCRC) CRS

Eldoret, , Kenya

Site Status NOT_YET_RECRUITING

12501, Kenya Medical Research Institute/Walter Reed Project Clinical Research Center (KEMRI/WRP) CRS

Kericho, , Kenya

Site Status RECRUITING

30301, Blantyre CRS

Blantyre, , Malawi

Site Status NOT_YET_RECRUITING

12001, Malawi CRS

Lilongwe, , Malawi

Site Status NOT_YET_RECRUITING

32078, Nutrición-Mexico CRS

Mexico City, , Mexico

Site Status NOT_YET_RECRUITING

11301, Barranco CRS

Lima, , Peru

Site Status NOT_YET_RECRUITING

31985, Socios En Salud Sucursal Perú CRS

Lima, , Peru

Site Status NOT_YET_RECRUITING

31981, TB HIV Innovations and Clinical Research Foundation Corp.

Cavite, , Philippines

Site Status NOT_YET_RECRUITING

31793, South African Tuberculosis Vaccine Initiative (SATVI) CRS

Cape Town, , South Africa

Site Status RECRUITING

31792, University of Cape Town Lung Institute (UCTLI) CRS

Cape Town, , South Africa

Site Status RECRUITING

31422, CAPRISA eThekwini CRS

Durban, , South Africa

Site Status NOT_YET_RECRUITING

11201, Durban International CRS

Durban, , South Africa

Site Status NOT_YET_RECRUITING

12301, Soweto ACTG CRS

Johannesburg, , South Africa

Site Status NOT_YET_RECRUITING

11101, University of the Witwatersrand Helen Joseph (WITS HJH) CRS

Johannesburg, , South Africa

Site Status NOT_YET_RECRUITING

31684, Rustenburg CRS

Rustenburg, , South Africa

Site Status RECRUITING

31784, Chiang Mai University HIV Treatment (CMU HIV Treatment) CRS

Chiang Mai, , Thailand

Site Status NOT_YET_RECRUITING

5116 Chiangrai Prachanukroh Hospital NICHD CRS

Chiang Rai, , Thailand

Site Status RECRUITING

31802, Thai Red Cross AIDS Research Centre (TRC-ARC) CRS

Pathum Wan, , Thailand

Site Status NOT_YET_RECRUITING

12401, Joint Clinical Research Centre (JCRC)/Kampala CRS

Kampala, , Uganda

Site Status NOT_YET_RECRUITING

32483 National Lung Hospital CRS

Hanoi, , Vietnam

Site Status NOT_YET_RECRUITING

30313, Milton Park CRS

Harare, , Zimbabwe

Site Status NOT_YET_RECRUITING

Countries

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Botswana Brazil Haiti India Kenya Malawi Mexico Peru Philippines South Africa Thailand Uganda Vietnam Zimbabwe

Central Contacts

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Radojka Savic, PharmD, PhD

Role: CONTACT

Phone: 415-502-0640

Email: [email protected]

Kelly Dooley, MD, PhD

Role: CONTACT

Phone: 615-322-8972

Email: [email protected]

Facility Contacts

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Unoda Chakalisa

Role: primary

Rita Lira

Role: primary

Brenda Hoagland

Role: primary

Samuel Pierre

Role: primary

Yvetot Joseph

Role: primary

Nishi Suryavanshi

Role: primary

Voila Kirui

Role: primary

Samwel Chirchir

Role: primary

Dumisile Huwa

Role: primary

Thokozani Makuhunga

Role: primary

Brenda Crabtree

Role: primary

Consuelo Tristan

Role: primary

Bruno Martel Chavez

Role: primary

Maria Gler

Role: primary

Lynnett Stone

Role: primary

Tammy Krige

Role: primary

Nivashnee Naicker

Role: primary

Rosie Mngqibisa

Role: primary

Suri Moonsamy

Role: primary

Betty Matome

Role: primary

Tiro Dinake

Role: primary

Daralak Tavornprasit

Role: primary

Pra-ornsuda Sukrakanchana

Role: primary

Parawee Thongpaeng

Role: primary

Sandra Rwambuya

Role: primary

Tran Viet Ha

Role: primary

Patience Sibanda

Role: primary

References

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Harrison LJ, Velasquez GE, Kempker RR, Imperial MZ, Nuermberger E, Dorman SE, Ignatius E, Granche J, Phillips PPJ, Furin J, Yang E, Foley C, Chiambah S, Rogers R, Van Grack A, Roa J, Shenje J, Nerette S, Kanyama C, Kyeyune RB, Mendoza-Ticona A, Murtaugh W, Foraida S, Goth M, Vernon A, Dooley KE, Savic RM. ACTG A5409 (RAD-TB): Study protocol for a phase 2 randomized, adaptive, dose-ranging, open-label trial of novel regimens for the treatment of pulmonary tuberculosis. Trials. 2025 Aug 15;26(1):291. doi: 10.1186/s13063-025-08973-w.

Reference Type DERIVED
PMID: 40817073 (View on PubMed)

Harrison L, Velasquez GE, Kempker RR, Imperial MZ, Nuermberger E, Dorman SE, Ignatius E, Granche J, Phillips PP, Furin J, Yang E, Foley C, Chiambah S, Rogers R, Van Grack A, Roa J, Shenje J, Nerette S, Kanyama C, Kyeyune RB, Mendoza-Ticona A, Murtaugh W, Foraida S, Goth M, Vernon A, Dooley KE, Savic RM. ACTG A5409 (RAD-TB): Study Protocol for a Phase 2 Randomized, Adaptive, Dose-Ranging, Open-Label Trial of Novel Regimens for the Treatment of Pulmonary Tuberculosis. Res Sq [Preprint]. 2025 Mar 26:rs.3.rs-5931694. doi: 10.21203/rs.3.rs-5931694/v1.

Reference Type DERIVED
PMID: 40195983 (View on PubMed)

Other Identifiers

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DOH-27-032024-5399

Identifier Type: OTHER

Identifier Source: secondary_id

A5409

Identifier Type: -

Identifier Source: org_study_id