Effects of Lactoferrin at Two Doses vs. Active Control on Markers of Immune Function

NCT ID: NCT06012669

Last Updated: 2025-06-11

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

NA

Total Enrollment

66 participants

Study Classification

INTERVENTIONAL

Study Start Date

2023-09-06

Study Completion Date

2023-12-27

Brief Summary

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The purpose of this study is to evaluate the impact of 28-day supplementation with high-dose human recombinant lactoferrin, low-dose human recombinant lactoferrin, and an active control product formulated with bovine lactoferrin on indicators of immunity in healthy males and females.

Detailed Description

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Samples will be retained for possible analyses such as lactoferrin absorption, distribution, metabolism, and excretion (ADME) and gut microbiota.

Conditions

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Immune Health

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

PARALLEL

Primary Study Purpose

OTHER

Blinding Strategy

QUADRUPLE

Participants Caregivers Investigators Outcome Assessors

Study Groups

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Low Dose

0.34 g Recombinant Human Lactoferrin

Group Type EXPERIMENTAL

Low dose Human Lactoferrin

Intervention Type DIETARY_SUPPLEMENT

Dietary Supplement and Food Ingredient

High Dose

3.4 g Recombinant Human Lactoferrin

Group Type EXPERIMENTAL

High dose Human Lactoferrin

Intervention Type DIETARY_SUPPLEMENT

Dietary Supplement and Food Ingredient

Active Control

3.4 g Bovine Lactoferrin

Group Type ACTIVE_COMPARATOR

Active Control Bovine Lactoferrin

Intervention Type DIETARY_SUPPLEMENT

Dietary Supplement and Food Ingredient

Interventions

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High dose Human Lactoferrin

Dietary Supplement and Food Ingredient

Intervention Type DIETARY_SUPPLEMENT

Low dose Human Lactoferrin

Dietary Supplement and Food Ingredient

Intervention Type DIETARY_SUPPLEMENT

Active Control Bovine Lactoferrin

Dietary Supplement and Food Ingredient

Intervention Type DIETARY_SUPPLEMENT

Eligibility Criteria

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Inclusion Criteria

1. Individual is male or premenopausal female (approximately half males and half females), 18 to 45 years of age, inclusive.
2. Individual has a BMI of ≥18.50 and \<30.00 kg/m2.
3. Individual is judged to be in good health based on medical history and routine laboratory tests.
4. Individual agrees to abstain from alcohol consumption and vigorous physical activity for at least 24 h prior to all test visits. According to the World Health Organization (WHO), vigorous physical activity may include running, fast cycling, fast swimming, or moving heavy objects.
5. Individual understands the study procedures, and is willing to complete them, and signs forms documenting informed consent to participate in the study and authorization for release of relevant protected health information to the study Investigator.

Exclusion Criteria

1. Individual is unwilling to avoid consumption of all dietary supplements (other than provided study products and multivitamin/mineral/calcium supplements) within 14 days of visit (day 0) and throughout the study period.
2. Individual is a chronic user of nicotine products.
3. Individual has a score \<7 on the Vein Access Scale.
4. Individual has a positive test for illicit drugs on the urine drug screen.
5. Individual has a clinically significant medical condition that, in the opinion of the Investigator, could interfere with the interpretation of the study results.
6. Individual has an autoimmune disorder (e.g., type 1 diabetes, rheumatoid arthritis, Graves disease, multiple sclerosis, lupus, etc.).
7. Individual has a gastrointestinal condition that would potentially interfere with absorption of the study product (e.g., inflammatory bowel disease, celiac disease, history of gastric bypass surgery, chronic gastritis).
8. Individual has used medication or over-the-counter products (including herbal and dietary supplements) that, in the opinion of the investigator, could interfere with the absorption and metabolism of lactoferrin or affect inflammatory or immune function markers including, but not limited to, aspirin, anti-inflammatories, antacids, histamine 2 receptor antagonists, laxatives, stool softeners, and proton-pump inhibitors.
9. Individual has had a weight change of ±4.5 kg (10 lbs.) in the previous 3 months.
10. Individual has extreme dietary habits (e.g., Atkins, vegan, very low carbohydrate diet).
11. Individual has history of bariatric surgery, is currently taking a weight loss drug, or is actively attempting to lose or gain body weight.
12. Individual has an active infection or signs/symptoms of an infection. Test visits will be rescheduled to allow the subject to be symptom-free of any type of systemic infection for at least five days.
13. Individual has recent use of antibiotics (within 7 days of visit 2, day 0) and throughout the study period. The test days will be rescheduled to allow the subject to wash out the antibiotic for at least 7 days.
14. Individual has uncontrolled hypertension (systolic blood pressure ≥160 mm Hg and/or diastolic blood pressure ≥100 mm Hg) at screening.
15. Individual has a history of cancer in the prior 2 years, except non-melanoma skin cancer or carcinoma in situ of the cervix.
16. Individual has an allergy or sensitivity to any components of the study products.
17. Individual has an allergy or sensitivity to yeast.
18. Individual is a female who is pregnant, planning to be pregnant during the study period, lactating, or is of childbearing potential and is unwilling to commit to the use of a medically approved form of contraception throughout the study period.
19. Individual has been exposed to any non-registered drug product within 30 days of the screening visit.
20. Individual has a current or recent history (past 12 months of screening) or strong potential for illicit drug or excessive alcohol intake defined as \>14 drinks per week (1 drink = 12 oz beer, 5 oz wine, or 1.5 oz hard liquor).
21. Individual has a condition the Investigator believes would interfere with his/her/their ability to provide informed consent, comply with the study protocol, which might confound the interpretation of the study results or put the person at undue risk.
Minimum Eligible Age

18 Years

Maximum Eligible Age

45 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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Helaina Inc.

INDUSTRY

Sponsor Role collaborator

Midwest Center for Metabolic and Cardiovascular Research

OTHER

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Kevin Maki, PhD

Role: STUDY_DIRECTOR

MB Clinical Research & Consulting, LLC

Locations

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Suncoast Research

Miami, Florida, United States

Site Status

Health Awareness

Port Saint Lucie, Florida, United States

Site Status

Countries

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United States

References

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Peterson RD, Guarneiri LL, Adams CG, Wilcox ML, Clark AJ, Rudemiller NP, Maki KC, Malinczak CA. A Randomized, Double-Blind, Controlled Trial to Assess the Effects of Lactoferrin at Two Doses vs. Active Control on Immunological and Safety Parameters in Healthy Adults. Int J Toxicol. 2025 Jan-Feb;44(1):12-28. doi: 10.1177/10915818241293723. Epub 2024 Oct 28.

Reference Type BACKGROUND
PMID: 39465888 (View on PubMed)

Other Identifiers

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MB-2305

Identifier Type: -

Identifier Source: org_study_id

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