No-biopsy Approach in Celiac Disease: Cut-off Points for IgA Anti-tissue Transglutaminase Assays

NCT ID: NCT05983978

Last Updated: 2025-08-06

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

RECRUITING

Total Enrollment

150 participants

Study Classification

OBSERVATIONAL

Study Start Date

2023-01-02

Study Completion Date

2026-03-31

Brief Summary

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The main objective of this multicenter and observational study is to define the optimal threshold of different commercially available IgA anti-transglutaminase (tTG-IgA) antibody assays for celiac disease diagnosis (CD) avoiding the need for an intestinal biopsy.

The main questions to be answered are:

* Is the anti-tTG-IgA titer cut-off above 10 times the upper limit of normal (ULN) useful in all anti-tTG IgA assays?
* Is the diagnostic performance of the newly defined cut-offs of anti-tTG-IgA the same in all the evaluated assays?
* Is the dynamic of the anti-tTG-IgA levels after the introduction of the gluten-free diet (GFD) similar across the different assays included in the study?

This is a prospective multicenter study that will enroll pediatric and adult patients with new-onset CD during the years 2023 and 2024. Serum from these patients will be collected for the determination of anti-tTG-IgA according to the local methodology (participating hospital) and by the anti-tTG IgA assays most commonly used in our country, which will be centralized in the same reference center (Hospital Universitario La Paz).

Detailed Description

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Conditions

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Celiac Disease

Study Design

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Observational Model Type

COHORT

Study Time Perspective

PROSPECTIVE

Eligibility Criteria

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Inclusion Criteria

* Patients, on gluten-containing diet, with TGt-IgA antibodies \> Upper Limit of Normal (ULN) and intestinal biopsy confirming celiac disease (CD).
* Patients with TCRγδ+ \>10% combined with decrease NK-like intraepithelial lymphocytes in the intestinal epithelium.
* Paediatric patients who meet ESPGHAN criteria (TGt-IgA antibodies \>10xULN) without duodenal biopsy performed.

Exclusion Criteria

* Patients already diagnosed with CD.
* Patients who refused duodenal biopsy.
* Patients with primary or secondary immunodeficiencies.
* Patients with malignancy.
* Recruited patients whose remaining serum cannot be sent to the reference centre, whose biopsies are of poor quality or who nod not sign the informed consent from.
Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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Hospital Universitari Mutua Terrassa

UNKNOWN

Sponsor Role collaborator

Hospital Sant Joan de Deu

OTHER

Sponsor Role collaborator

Hospital Universitario Ramon y Cajal

OTHER

Sponsor Role collaborator

Hospital Universitario Lucus Augusti

OTHER

Sponsor Role collaborator

Hospital Universitario Fundación Jiménez Díaz

OTHER

Sponsor Role collaborator

Instituto de Investigación Sanitaria Hospital Clínico San Carlos

UNKNOWN

Sponsor Role collaborator

Instituto de Investigación Hospital Universitario La Paz

OTHER

Sponsor Role lead

Responsible Party

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Marta Molero

Doctor

Responsibility Role PRINCIPAL_INVESTIGATOR

Principal Investigators

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Marta Molero-Luis, Dr

Role: PRINCIPAL_INVESTIGATOR

Hospital Universitario La Paz

Locations

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Hospital Mutua Terrassa

Terrassa, Barcelona, Spain

Site Status RECRUITING

Hospital Universitario Lucus Augusti

Lugo, Lugo, Spain

Site Status RECRUITING

Hospital Universitario La Paz

Madrid, Madrid, Spain

Site Status RECRUITING

Hospital Sant Joan de Deu

Barcelona, , Spain

Site Status RECRUITING

Hospital Ramon y Cajal

Madrid, , Spain

Site Status RECRUITING

Hospital Universitario Fundación Jimenez Diaz

Madrid, , Spain

Site Status RECRUITING

Countries

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Spain

Central Contacts

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Marta Molero-Luis, Dr

Role: CONTACT

0034660065755

Concepción Núñez Pardo de Vera, Dr

Role: CONTACT

Facility Contacts

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Albert Martin Cardona

Role: primary

Julia María Cabo del Riego

Role: primary

Marta Molero-Luis, Dr

Role: primary

Elisabet Poyatos

Role: primary

Garbiñe Roy Ariño

Role: primary

María José Martínez Becerra

Role: primary

Other Identifiers

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PI-5473

Identifier Type: -

Identifier Source: org_study_id

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