Maternal Determinants of Infant Immunity to Pertussis

NCT ID: NCT05856396

Last Updated: 2026-01-13

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

PHASE4

Total Enrollment

200 participants

Study Classification

INTERVENTIONAL

Study Start Date

2023-09-12

Study Completion Date

2025-12-23

Brief Summary

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The overall objective of the project is to identify the determinants of antibody-mediated immunity in infants born to mothers immunized during pregnancy. Using maternal pertussis immunization as a model, the project will identify key predictors and potential determinants of vaccine responses in pregnant women, of the transfer of maternal antibodies to the newborn and of vaccine responses in infants. A systems biology approach will be used to delineate pre-vaccination and post-vaccination cellular and molecular correlates of the immune response to pertussis immunization in peripheral blood and in breastmilk.

Detailed Description

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The overall objective of the project is to identify the determinants of antibody-mediated immunity to pertussis in infants born to mothers immunized during pregnancy. Three specific objectives will be targeted:

1. Determine the impact of pregnancy on the quality of antibody response to pertussis immunization and identify immune predictors of vaccine responses in pregnant and non-pregnant women.
2. Identify immune predictors of the transfer of maternal antibodies to the newborn and the presence of antibody in breastmilk following pertussis immunization during pregnancy.
3. Determine the impact of maternal antibodies on the quality of antibody response to pertussis immunization in infants born to mothers immunized or not immunized during pregnancy and identify immune predictors of vaccine responses in the first months of life.

To reach these objectives, 40 non-pregnant and 80 pregnant women will be recruited into the study and vaccinated with a single dose of a pertussis containing vaccine (Triaxis). Blood samples will be collected from:

* non-pregnant women: before vaccination, and day 1/7/28 and month 5 post-vaccination.
* pregnant women: before vaccination, day 1/7/28 post-vaccination, at delivery, and week 6/12 post-delivery. At week 6/12 post-delivery, breast milk samples will be collected as well.

At delivery, a placenta fragment will be collected.

In addition, infants 2-3 months old born either from mothers who were not vaccinated against pertussis during pregnancy (n=40) or born from mothers who were vaccinated against pertussis during pregnancy (n=80) will be recruited in the study. Infants will be vaccinated with three doses of a pertussis containing vaccine (Vaxelis), each one month apart starting from 2-3 months of age. Blood samples will be collected from:

* infants from vaccinated mothers: cord blood, before 1st vaccine dose, day 1 post 1st vaccine dose, before 3rd vaccine dose, and day 28 post 3rd vaccine dose.
* infants from unvaccinated mothers: before 1st vaccine dose, day 1 post 1st vaccine dose, before 3rd vaccine dose, and day 28 post 3rd vaccine dose.

Conditions

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Vaccination; Infection Maternal-Fetal Relations Pertussis Immunoglobulins

Study Design

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Allocation Method

NON_RANDOMIZED

Intervention Model

PARALLEL

* Non pregnant and pregnant women will be recruited in parallel
* Infants born to Tdap vaccinated-mothers (enrolled or not in the study) or not vaccinated mothers will be recruited
Primary Study Purpose

BASIC_SCIENCE

Blinding Strategy

SINGLE

Outcome Assessors
Researchers analyzing the immunological outcomes will be blinded to the groups of the subjects (pregnant vs non pregnant ; infants)

Study Groups

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Pregnant women

Pregnant women will receive one dose of Pertussis-containing vaccine during pregnancy.

Group Type ACTIVE_COMPARATOR

Triaxis® (Pertussis-containing vaccine)

Intervention Type BIOLOGICAL

Triaxis® (Pertussis-containing vaccine) will be administered:

* in non-pregnant women presenting to the Travel and Vaccine clinic for pertussis immunization only or hospital member staff requiring Tetanus Toxoid (TT)-booster immunisation
* in Pregnant women between 16 and 29 weeks of gestation.

Non pregnant women

Non-Pregnant women will receive one dose of Pertussis-containing vaccine.

Group Type ACTIVE_COMPARATOR

Triaxis® (Pertussis-containing vaccine)

Intervention Type BIOLOGICAL

Triaxis® (Pertussis-containing vaccine) will be administered:

* in non-pregnant women presenting to the Travel and Vaccine clinic for pertussis immunization only or hospital member staff requiring Tetanus Toxoid (TT)-booster immunisation
* in Pregnant women between 16 and 29 weeks of gestation.

Infants born to Tdap-vaccinated mothers

Infants whose mothers have been immunized during pregnancy with Tdap vaccine. Infants will receive three doses of Pertussis-containing vaccine (with 28 days interval starting at two months of age).

Group Type ACTIVE_COMPARATOR

Vaxelis® (Pertussis-containing vaccine)

Intervention Type BIOLOGICAL

Vaxelis® (Hexavalent vaccine) will be proposed in infants at 8, 12 and 16 weeks of life.

Infants born to non Tdap-vaccinated mothers

Infants whose mothers have not been immunized during pregnancy with Tdap vaccine.

Infants will receive three doses of Pertussis-containing vaccine (with 28 days interval starting at two months of age).

Group Type ACTIVE_COMPARATOR

Vaxelis® (Pertussis-containing vaccine)

Intervention Type BIOLOGICAL

Vaxelis® (Hexavalent vaccine) will be proposed in infants at 8, 12 and 16 weeks of life.

Interventions

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Triaxis® (Pertussis-containing vaccine)

Triaxis® (Pertussis-containing vaccine) will be administered:

* in non-pregnant women presenting to the Travel and Vaccine clinic for pertussis immunization only or hospital member staff requiring Tetanus Toxoid (TT)-booster immunisation
* in Pregnant women between 16 and 29 weeks of gestation.

Intervention Type BIOLOGICAL

Vaxelis® (Pertussis-containing vaccine)

Vaxelis® (Hexavalent vaccine) will be proposed in infants at 8, 12 and 16 weeks of life.

Intervention Type BIOLOGICAL

Eligibility Criteria

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Inclusion Criteria

* For non-pregnant \& pregnant women Age between 18 and 45 years Eligible for Tdap vaccination
* For infants Born to mothers vaccinated or not with Tdap Vaccinated with hexavalent vaccine Age between 2 and 3 months

Exclusion Criteria

* For pregnant and non-pregnant women

* Inability to understand the nature and extent of the study and the procedures required
* Grade III/IV anemia,
* Acute infection at the time of immunization
* Chronic infections such as Hepatitis B Virus (HBV), Hepatitis C Virus (HCV) or Human Immunodeficiency Virus (HIV) infection, acute toxoplasmosis
* Current or recent use of immunosuppressive drugs
* Active neoplasia
* Other vaccine(s) administered at the same time as Tdap vaccination (wash out of 4 weeks after others vaccinations and 28 days after Tdap vaccination )
* For pregnant women

* Risk of premature delivery or intrauterine growth retardation
* Twin or triplet pregnancies
* For non-pregnant women Last Tdap vaccination \< 12 months before

For infants:

* Infants born before 35 weeks of gestation
* Birthweight below 2.5 kg,
* Severe neonatal distress
* Serious congenital abnormalities or congenital infection.
Minimum Eligible Age

2 Months

Maximum Eligible Age

45 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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Centre Hospitalier Universitaire Saint Pierre

OTHER

Sponsor Role lead

Université Libre de Bruxelles

OTHER

Sponsor Role collaborator

Responsible Party

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Tessa Goetghebuer

Head of Clinic

Responsibility Role PRINCIPAL_INVESTIGATOR

Locations

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CHU Saint-Pierre

Brussels, , Belgium

Site Status

Countries

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Belgium

References

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Marchant A, Sadarangani M, Garand M, Dauby N, Verhasselt V, Pereira L, Bjornson G, Jones CE, Halperin SA, Edwards KM, Heath P, Openshaw PJ, Scheifele DW, Kollmann TR. Maternal immunisation: collaborating with mother nature. Lancet Infect Dis. 2017 Jul;17(7):e197-e208. doi: 10.1016/S1473-3099(17)30229-3. Epub 2017 Apr 19.

Reference Type BACKGROUND
PMID: 28433705 (View on PubMed)

Gunn BM, Alter G. Modulating Antibody Functionality in Infectious Disease and Vaccination. Trends Mol Med. 2016 Nov;22(11):969-982. doi: 10.1016/j.molmed.2016.09.002. Epub 2016 Oct 15.

Reference Type BACKGROUND
PMID: 27756530 (View on PubMed)

Jennewein MF, Alter G. The Immunoregulatory Roles of Antibody Glycosylation. Trends Immunol. 2017 May;38(5):358-372. doi: 10.1016/j.it.2017.02.004. Epub 2017 Apr 3.

Reference Type BACKGROUND
PMID: 28385520 (View on PubMed)

Jennewein MF, Abu-Raya B, Jiang Y, Alter G, Marchant A. Transfer of maternal immunity and programming of the newborn immune system. Semin Immunopathol. 2017 Nov;39(6):605-613. doi: 10.1007/s00281-017-0653-x. Epub 2017 Oct 2.

Reference Type BACKGROUND
PMID: 28971246 (View on PubMed)

Jennewein MF, Goldfarb I, Dolatshahi S, Cosgrove C, Noelette FJ, Krykbaeva M, Das J, Sarkar A, Gorman MJ, Fischinger S, Boudreau CM, Brown J, Cooperrider JH, Aneja J, Suscovich TJ, Graham BS, Lauer GM, Goetghebuer T, Marchant A, Lauffenburger D, Kim AY, Riley LE, Alter G. Fc Glycan-Mediated Regulation of Placental Antibody Transfer. Cell. 2019 Jun 27;178(1):202-215.e14. doi: 10.1016/j.cell.2019.05.044. Epub 2019 Jun 13.

Reference Type BACKGROUND
PMID: 31204102 (View on PubMed)

Jennewein MF, Kosikova M, Noelette FJ, Radvak P, Boudreau CM, Campbell JD, Chen WH, Xie H, Alter G, Pasetti MF. Functional and structural modifications of influenza antibodies during pregnancy. iScience. 2022 Mar 16;25(4):104088. doi: 10.1016/j.isci.2022.104088. eCollection 2022 Apr 15.

Reference Type BACKGROUND
PMID: 35402869 (View on PubMed)

Tsang JS. Utilizing population variation, vaccination, and systems biology to study human immunology. Trends Immunol. 2015 Aug;36(8):479-93. doi: 10.1016/j.it.2015.06.005. Epub 2015 Jul 14.

Reference Type BACKGROUND
PMID: 26187853 (View on PubMed)

Other Identifiers

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B0762022220909

Identifier Type: -

Identifier Source: org_study_id

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