Circular RNA and Chemerin in Breast Cancer Patient

NCT ID: NCT05771337

Last Updated: 2023-03-16

Basic Information

• Recruitment Status: NOT_YET_RECRUITING
• Total Enrollment: 80 participants
• Study Classification: OBSERVATIONAL
• Study Start Date: 2023-10-31
• Study Completion Date: 2025-12-31

Brief Summary

This study aimed to evaluate the role of serum chemerin as a biomarker of breast cancer.

and To investigate the diagnostic value of hsa_circ_0001785 (Circ-ELP3) and hsa_circ_100219 (Circ-FAF1) in serum samples of breast cancer patients to find out whether these circRNAs can utilize as a diagnostic and prognostic biomarker for human breast cancer assessment and make Correlation between serum chemerin levels , serum Circ-FAF1 , serum CircELP3 and classical tumor markers (CEA + CA15-3).

andTo elucidate if there is a difference between metastatic and non-metastatic cases as regard serum chemerin levels, serum Circ-FAF1 or Circ-ELP3.

Detailed Description

Breast cancer is the most common cancer among women and despite advances in therapy over the past few decades, drug resistance, recurrence, metastasis and the heterogeneity of the cancer continue to be a problem in successful management of the diseases(Hong et al ,2019) .

Circular RNAs (CircRNAs) are single-stranded, covalently closed RNA molecules that are ubiquitous across species ranging from viruses to mammals(Zhou et al 3 .,2020). circRNAs can be divided into two categories including coding and noncoding circRNAs(Li et al., 2019) A new subtype of RNAs is circular RNAs, single-strand RNA molecules with less than 100 to more than 4,000 nucleotides (Lu et al., 2016) and a covalently closedloop structure. These molecules are produced through a backsplicing mechanism, in which the downstream 5'-end of the splice donor joins the upstream 3'-end splice acceptor and forms a product with a circular structure (wang et al., 2017).

Chemerin is a leukocyte chemoattractant widely expressed in many tissues and is known to recruit innate leukocytes.

Recently, more and more studies focus on the relationship between chemerin and cancers and found that chemerin acts an essential role in cancers.(Shin et al,2018) Chemerin expression is different depending on the tumor type (Zhang Y et al, 2013) the clinical significance of chemerin in breast cancer has been only reported by a very limited number of studies (EL Sagheer et al,2018).

Conditions

Breast Cancer

Study Design

• Observational Model Type: CASE_CONTROL
• Study Time Perspective: RETROSPECTIVE

Study Groups

breast cancer leasion

blood sample

Intervention Type: DIAGNOSTIC_TEST

blood sample

benign breast lesion

blood sample

Intervention Type: DIAGNOSTIC_TEST

blood sample

Interventions

blood sample

blood sample

Intervention Type: DIAGNOSTIC_TEST

Eligibility Criteria

Inclusion Criteria

• histopathological diagnosis of breast cancer

Exclusion Criteria

• history of other types of cancers
• history of HIV
• history of any therapeutic interventions in form of chemo or hormonal therapy before specimen collection

• Minimum Eligible Age: 18 Years
• Maximum Eligible Age: 60 Years
• Eligible Sex: FEMALE
• Accepts Healthy Volunteers: Yes

Sponsors

Assiut University

OTHER

Sponsor Role: lead

Responsible Party

Sandy Nashat Sorial Shokrey

demonstrator in biochemestry departement

Responsibility Role: PRINCIPAL_INVESTIGATOR

Central Contacts

sandy nashat sorial

Role: CONTACT

sandybsbsa@yahoo.com

01200693648

abdelhaleem ali abdelhaleem

Role: CONTACT

References

Song Y, Zhu X, Lin Z, Luo L, Wen D. The potential value of serum chemerin in patients with breast cancer. Sci Rep. 2021 Mar 22;11(1):6564. doi: 10.1038/s41598-021-85986-w.

Reference Type: BACKGROUND

PMID: 33753802 (View on PubMed)

Omid-Shafaat R, Moayeri H, Rahimi K, Menbari MN, Vahabzadeh Z, Hakhamaneshi MS, Nouri B, Ghaderi B, Abdi M. Serum Circ-FAF1/Circ-ELP3: A novel potential biomarker for breast cancer diagnosis. J Clin Lab Anal. 2021 Nov;35(11):e24008. doi: 10.1002/jcla.24008. Epub 2021 Sep 21.

Reference Type: BACKGROUND

PMID: 34545638 (View on PubMed)

Other Identifiers

biomarkers in breast cancer

Identifier Type: -

Identifier Source: org_study_id