Trans-RosaLEE Study: a Biomarker-directed, Translational Study

NCT ID: NCT05529862

Last Updated: 2026-01-22

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

ACTIVE_NOT_RECRUITING

Clinical Phase

NA

Total Enrollment

115 participants

Study Classification

INTERVENTIONAL

Study Start Date

2023-06-20

Study Completion Date

2028-11-01

Brief Summary

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Hormone receptor (HR)-positive and HER2-negative (HR+/HER2-) metastatic/advanced breast cancer (mBC) is a major public health issue. During the last decades, a therapeutic challenge was to overcome the tumor's resistance to endocrine therapy (ET). Thanks to a better understanding of the molecular mechanisms of this resistance, effective new treatments have been developed, such as Kisqali® (ribociclib), a molecularly targeted therapy. This treatment blocks the growth and division of cancer cells by blocking proteins called CDK4/6 located inside the cell. This treatment, taken in combination with ET, blocks the harmful effect of hormones (estrogen) on cancer cell proliferation, and represent the standard first-line treatment of patients with HR+/HER2- mBC.

But, as with any treatment, it is expected that some patients will have a good response and their disease will be stabilized or even in remission, while other patients will not benefit from treatment and will relapse. In order to make progress, it is necessary to identify pre-therapeutic markers predictive of response to this treatment and the molecular mechanisms of this resistance set up by the tumor before or under the effect of the treatment.

The Trans-RosaLEE study aims to fill this gap by providing high-throughput molecular profiling (DNA and RNA) of a collection of tumor and blood samples from patients with RH+/HER2- mBC scheduled to start treatment with Kisqali® + ET. Samples will be collected just prior to initiation of therapy (pre-therapy) and just after discontinuation of therapy in the event of disease progression (post-therapy).

The main objectives of the TransRosaLEE study are :

* to determine if Kisqali® + ET treatment causes changes in the DNA and/or RNA genes of tumor;
* to identify whether there is a molecular signature that would predict clinical outcome of patients treated with Kisqali® + ET (tumor response, survival);
* to identify alterations in tumor's genes that could be targeted by a specific treatment and that would allow, in case of progression of the disease, to set up a new adapted treatment.

The TransRosaLEE study is a collaborative study between the Paoli-Calmettes Institute (France, Marseille) and the pharmaceutical group Novartis. It will take place in up to 90 healthcare institutions in France, and 241 patients will be enrolled. It is closely linked to the non-interventional study RosaLEE promoted by Novartis.

Detailed Description

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Conditions

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Advanced or Metastatic Breast Cancer (BC)

Study Design

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Allocation Method

NA

Intervention Model

SINGLE_GROUP

Primary Study Purpose

BASIC_SCIENCE

Blinding Strategy

NONE

Study Groups

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Locally advanced or metastatic Breast Cancer Women

Study Procedures:

* Additional tumour sampling during the pre-treatment biopsy scheduled as per routine care,
* A post-treatment tumour biopsy,
* One pre-treatment and one post-treatment blood sampling.

Group Type EXPERIMENTAL

Pre-treatment biopsy

Intervention Type GENETIC

Pre-treatment fragments will be collected during the biopsy visit organised as part of routine medical practice, prior to the start of treatment with ribociclib + ET

Post treatment biopsy

Intervention Type GENETIC

Post-treatment fragments will be collected during a biopsy visit specifically planned for Trans-RosaLEE study.

Pre treatment blood sampling

Intervention Type GENETIC

Sampling of 4 EDTA Tubes (4ml) and 2 Streck tubes (10ml)

Post treatment blood sampling

Intervention Type GENETIC

Sampling of 2 Streck tubes (10ml)

Interventions

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Pre-treatment biopsy

Pre-treatment fragments will be collected during the biopsy visit organised as part of routine medical practice, prior to the start of treatment with ribociclib + ET

Intervention Type GENETIC

Post treatment biopsy

Post-treatment fragments will be collected during a biopsy visit specifically planned for Trans-RosaLEE study.

Intervention Type GENETIC

Pre treatment blood sampling

Sampling of 4 EDTA Tubes (4ml) and 2 Streck tubes (10ml)

Intervention Type GENETIC

Post treatment blood sampling

Sampling of 2 Streck tubes (10ml)

Intervention Type GENETIC

Eligibility Criteria

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Inclusion Criteria

1. Patients included in the RosaLEE study.
2. Patients having read and signed the ICF relative to Trans-RosaLEE.
3. Tumour material: primary and/or metastatic tumour sample, either available as frozen and collected within 3 months before V0, or newly collected before ribociclib + ET treatment initiation.

Brain metastases and non-osteolytic bone metastases will be considered as non-collectable/biopsable.
4. Patient affiliated to the national "Social Security" regimen or beneficiary of this regimen.

Exclusion Criteria

1. Not enrolled in RosaLEE.
2. Brain metastasis and non-osteolytic bone metastases as only metastatic sites, if no available frozen tumour sample already collected within 3 months before V0.
3. Tumour material not collected before ribociclib + ET initiation.
4. Person subject to a legal protection measure (adult under guardianship, curatorship or safeguard of justice), or unable to give their consent.
Minimum Eligible Age

18 Years

Eligible Sex

FEMALE

Accepts Healthy Volunteers

No

Sponsors

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Novartis Pharmaceuticals

INDUSTRY

Sponsor Role collaborator

Institut Paoli-Calmettes

OTHER

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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François Bertucci, MD PhD

Role: PRINCIPAL_INVESTIGATOR

Institut Paoli-Calmettes

Locations

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Institut Paoli Calmettes

Marseille, , France

Site Status

Countries

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France

Other Identifiers

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TransRosaLEE-IPC 2021-075

Identifier Type: -

Identifier Source: org_study_id

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